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간세포성장인자가 하인두 편평세포암 세포주에서 Matrix Metalloproteinase(MMP)-2, 9과 Urokinase Type Plasmogen Activator에 미치는 영향

Other Titles
 Effect of HGF in Invasion of Hypopharyngeal Squamous Cell Carcinoma Cell Line 
Authors
 김철호  ;  문성균  ;  배준호  ;  이재호  ;  최은창 
Citation
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.48(6) : 788-795, 2005 
Journal Title
Korean Journal of Otolaryngology(대한이비인후과학회지)
ISSN
 1225-035X 
Issue Date
2005
MeSH
Hepatocyte growth factor ; c-Met, Hypopharyngeal cancer ; Invasion ; Urokinase-type plasminogen activator ; Matrix metalloproteinase
Keywords
Hepatocyte growth factor ; c-Met, Hypopharyngeal cancer ; Invasion ; Urokinase-type plasminogen activator ; Matrix metalloproteinase
Abstract
Background and Objectives:Recent reports revealed that hepatocyte growth factor (HGF) is related to tumor invasion and metastasis. Activation of the urokinase-type plasminogen activator (u-PA)/plasmin proteolytic network and matrix metalloproteinase has been shown to play a key role in tumor invasion and dissemination of various malignancies. So we examined the effect of HGF/c-Met on MMP-2, 9 and u-PA in FaDu cell, a hypopharyngeal squamous cell carcinoma cell line.

Materials and Method:We performed RT-PCR and Western blot in FaDu. Tumor cell invasiveness was assessed by the membrane invasion assay (using Transwell chamber). To examine the role of MMP-2, 9 and the relation between HGF and MMP in the invasion of hypopharyngeal cancer, RT-PCR and zymography were performed in FaDu cells. We tested to confirm the HGF-mediated plasmin activation.

Results:The expressions of c-Met mRNA and protein were detected in the hypopharyngeal cell line while that of HGF was not. HGF markedly enhanced the invasion of cancer cells in a Transwell invasion chamber in a dose-dependent manner (p<0.05). The expression of MMP was detected in hypopharyngeal cancer cells and exogenous HGF slightly enhanced the induction of MMP-2 activity in zymogram analysis. The activity of u-PA was detected in FaDu and HGF (above 10 units/mL) enhanced the activity of u-PA (p<0.05).

Conclusion:These results suggest that HGF may play an important role in hypopharyngeal cancer through the activation of u-PA and matrix metalloproteinase.
Files in This Item:
T200500028.pdf Download
DOI
OAK-2005-02362
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Choi, Eun Chang(최은창)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/147287
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