Cited 139 times in
Derivation and characterization of new human embryonic stem cell lines: SNUhES1, SNUhES2, and SNUhES3
DC Field | Value | Language |
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dc.contributor.author | 김동욱 | - |
dc.date.accessioned | 2017-05-04T07:25:29Z | - |
dc.date.available | 2017-05-04T07:25:29Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1066-5099 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147271 | - |
dc.description.abstract | Here we report the derivation and characterization of new human embryonic stem cell (hESC) lines, SNUhES1, SNUhES2, and SNUhES3. These cells, established from the inner cell mass using an STO feeder layer, satisfy the criteria that characterize pluripotent hESCs: The cell lines express high levels of alkaline phosphatase, cell surface markers (such as SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), transcription factor Oct-4, and telomerase. When grafted into severe combined immunodeficient mice after prolonged proliferation, these cells maintained the developmental potentials to form derivatives of all three embryonic germ layers. The cell lines have normal karyotypes and distinct identities, revealed from DNA fingerprinting. Interestingly, analysis by electron microscopy clearly shows the morphological difference between undifferentiated and differentiated hESCs. Undifferentiated hESCs have a high ratio of nucleus to cytoplasm, prominent nucleoli, indistinct cell membranes, free ribosomes, and small mitochondria with a few crista, whereas differentiated cells retain irregular nuclear morphology, desmosomes, extensive cytoplasmic membranes, tonofilaments, and highly developed cellular organelles such as Golgi complex with secretory vesicles, endoplasmic reticulum studded with ribosomes, and large mitochondria. Existence of desmosomes and tonofilaments indicates that these cells differentiated into epithelial cells. When in vitro differentiation potentials of these cell lines into cardiomyocytes were examined, SNUhES3 was found to differentiate into cardiomyocytes most effectively. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | AlphaMed Press | - |
dc.relation.isPartOf | STEM CELLS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Differentiation/physiology* | - |
dc.subject.MESH | Cell Line/physiology* | - |
dc.subject.MESH | Cell Line/ultrastructure | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Membrane Proteins/analysis* | - |
dc.subject.MESH | Membrane Proteins/metabolism | - |
dc.subject.MESH | Myocytes, Cardiac/physiology | - |
dc.subject.MESH | Myocytes, Cardiac/ultrastructure | - |
dc.subject.MESH | Totipotent Stem Cells/physiology* | - |
dc.subject.MESH | Totipotent Stem Cells/ultrastructure | - |
dc.title | Derivation and characterization of new human embryonic stem cell lines: SNUhES1, SNUhES2, and SNUhES3 | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학교실) | - |
dc.contributor.googleauthor | Sun Kyung Oh | - |
dc.contributor.googleauthor | Hee Sun Kim | - |
dc.contributor.googleauthor | Hee Jin Ahn | - |
dc.contributor.googleauthor | Hye Won Seol | - |
dc.contributor.googleauthor | Yoon Young Kim | - |
dc.contributor.googleauthor | Yong Bin Park | - |
dc.contributor.googleauthor | Chul Jong Yoon | - |
dc.contributor.googleauthor | Dong-Wook Kim | - |
dc.contributor.googleauthor | Seok Hyun Kim | - |
dc.contributor.googleauthor | Shin Yong Moon | - |
dc.identifier.doi | 10.1634/stemcells.2004-0122 | - |
dc.contributor.localId | A00406 | - |
dc.relation.journalcode | J02683 | - |
dc.identifier.eissn | 1549-4918 | - |
dc.identifier.pmid | 15671144 | - |
dc.identifier.url | https://stemcellsjournals.onlinelibrary.wiley.com/doi/abs/10.1634/stemcells.2004-0122 | - |
dc.subject.keyword | Cell replacement therapy | - |
dc.subject.keyword | Derivation of hESCs | - |
dc.subject.keyword | Differentiation into cardiomyocytes | - |
dc.subject.keyword | EM analysis of hESCs | - |
dc.subject.keyword | Human embryonic stem cells | - |
dc.contributor.alternativeName | Kim, Dong Wook | - |
dc.citation.volume | 23 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 211 | - |
dc.citation.endPage | 219 | - |
dc.identifier.bibliographicCitation | STEM CELLS, Vol.23(2) : 211-219, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 48445 | - |
dc.type.rims | ART | - |
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