0 518

Cited 36 times in

Massively parallel sequencing of 17 commonly used forensic autosomal STRs and amelogenin with small amplicons.

DC Field Value Language
dc.contributor.author신경진-
dc.contributor.author양우익-
dc.contributor.author이환영-
dc.contributor.author정상은-
dc.contributor.author양인석-
dc.date.accessioned2017-02-27T07:46:27Z-
dc.date.available2017-02-27T07:46:27Z-
dc.date.issued2016-
dc.identifier.issn1872-4973-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/146986-
dc.description.abstractThe next-generation sequencing (NGS) method has been utilized to analyze short tandem repeat (STR) markers, which are routinely used for human identification purposes in the forensic field. Some researchers have demonstrated the successful application of the NGS system to STR typing, suggesting that NGS technology may be an alternative or additional method to overcome limitations of capillary electrophoresis (CE)-based STR profiling. However, there has been no available multiplex PCR system that is optimized for NGS analysis of forensic STR markers. Thus, we constructed a multiplex PCR system for the NGS analysis of 18 markers (13CODIS STRs, D2S1338, D19S433, Penta D, Penta E and amelogenin) by designing amplicons in the size range of 77-210 base pairs. Then, PCR products were generated from two single-sources, mixed samples and artificially degraded DNA samples using a multiplex PCR system, and were prepared for sequencing on the MiSeq system through construction of a subsequent barcoded library. By performing NGS and analyzing the data, we confirmed that the resultant STR genotypes were consistent with those of CE-based typing. Moreover, sequence variations were detected in targeted STR regions. Through the use of small-sized amplicons, the developed multiplex PCR system enables researchers to obtain successful STR profiles even from artificially degraded DNA as well as STR loci which are analyzed with large-sized amplicons in the CE-based commercial kits. In addition, successful profiles can be obtained from mixtures up to a 1:19 ratio. Consequently, the developed multiplex PCR system, which produces small size amplicons, can be successfully applied to STR NGS analysis of forensic casework samples such as mixtures and degraded DNA samples.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfFORENSIC SCIENCE INTERNATIONAL-GENETICS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmelogenin/genetics*-
dc.subject.MESHDNA/analysis-
dc.subject.MESHDNA/genetics-
dc.subject.MESHDNA Fingerprinting/methods*-
dc.subject.MESHFemale-
dc.subject.MESHForensic Anthropology-
dc.subject.MESHForensic Genetics/methods*-
dc.subject.MESHGenotype-
dc.subject.MESHHigh-Throughput Nucleotide Sequencing/methods*-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMicrosatellite Repeats*-
dc.subject.MESHMultiplex Polymerase Chain Reaction/methods-
dc.subject.MESHNucleic Acid Amplification Techniques/methods-
dc.titleMassively parallel sequencing of 17 commonly used forensic autosomal STRs and amelogenin with small amplicons.-
dc.typeArticle-
dc.publisher.locationNetherlands-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Forensic Medicine-
dc.contributor.googleauthorEun Hye Kim-
dc.contributor.googleauthorHwan Young Lee-
dc.contributor.googleauthorIn Seok Yang-
dc.contributor.googleauthorSang-Eun Jung-
dc.contributor.googleauthorWoo Ick Yang-
dc.contributor.googleauthorKyoung-Jin Shin-
dc.identifier.doi10.1016/j.fsigen.2016.01.001-
dc.contributor.localIdA02085-
dc.contributor.localIdA02300-
dc.contributor.localIdA03335-
dc.contributor.localIdA03614-
dc.relation.journalcodeJ00905-
dc.identifier.eissn1878-0326-
dc.identifier.pmid26799314-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1872497316300011-
dc.subject.keywordAutosomal STR-
dc.subject.keywordDegraded DNA-
dc.subject.keywordMixture-
dc.subject.keywordNext-generation sequencing-
dc.subject.keywordSequence variation-
dc.subject.keywordSmall-sized amplicon-
dc.contributor.alternativeNameShin, Kyoung Jin-
dc.contributor.alternativeNameYang, Woo Ick-
dc.contributor.alternativeNameLee, Hwan Young-
dc.contributor.alternativeNameJung, Sang Eun-
dc.contributor.affiliatedAuthorShin, Kyoung Jin-
dc.contributor.affiliatedAuthorYang, Woo Ick-
dc.contributor.affiliatedAuthorLee, Hwan Young-
dc.contributor.affiliatedAuthorJung, Sang Eun-
dc.citation.volume22-
dc.citation.startPage1-
dc.citation.endPage7-
dc.identifier.bibliographicCitationFORENSIC SCIENCE INTERNATIONAL-GENETICS, Vol.22 : 1-7, 2016-
dc.date.modified2017-02-24-
dc.identifier.rimsid46549-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biomedical Systems Informatics (의생명시스템정보학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.