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Biomarkers for Bisphosphonate-Related Osteonecrosis of the Jaw

Authors
 Jin-Woo Kim  ;  In-Ho Cha  ;  Sun-Jong Kim  ;  Myung-Rae Kim 
Citation
 CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH, Vol.18(2) : 281-291, 2016 
Journal Title
 CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH 
ISSN
 1523-0899 
Issue Date
2016
MeSH
Animals ; Biomarkers* ; Bisphosphonate-Associated Osteonecrosis of the Jaw* ; Disease Models, Animal ; Humans ; Osteoclasts ; Osteonecrosis ; RANK Ligand/analysis* ; Rats ; Rats, Sprague-Dawley ; Tartrate-Resistant Acid Phosphatase/analysis*
Keywords
biomarker ; bisphosphonate ; osteonecrosis of the jaw ; receptor activator of nuclear factor-κβ ligand ; tartrate-resistant acid phosphatase
Abstract
PURPOSE: The aim of this study was to investigate a possible biomarker for bisphosphonate-related osteonecrosis of the jaw (BRONJ) in an animal model. MATERIALS AND METHODS: Forty-eight Sprague-Dawley rats were randomly divided into the bisphosphonate group (n = 36), who were injected once a week with zoledronic acid, and the control group (n = 12), who were injected once a week with saline. After 6 weeks, surgical intervention was performed, and injections were continued up to 8 weeks. Rats in the bisphosphonate group were then further classified to the ONJ group, and the non-ONJ group, and biomarkers, including CTx, Glu-OC, TRACP 5b, RANKL, and OPG, were assessed at baseline (T0), at surgical intervention (T1), and at sacrifice (T2). Histomorphometric analysis for quantification of osteoclasts was performed. RESULTS: Repeated measures analysis of variance revealed that TRACP 5b levels and the RANKL/OPG ratio were significantly decreased over time in the ONJ group compared with the non-ONJ group (p < .05). At T2, the area under the curve was 0.807 for TRACP 5b (sensitivity: 88.9%, specificity 66.7% at cutoff) and 0.765 for the RANKL/OPG ratio (sensitivity: 77.8%, specificity 62.9% at cutoff). TRACP 5b showed a lower least significant change (29.6%) with lower intra-assay coefficient of variability (CV; 6.32%) and interassay CV (11.20%) compared with those of the RANKL/OPG ratio (39.27%) and showed a higher signal-to-noise ratio (2.76) than that of the RANKL/OPG ratio (1.62). N.Oc/T.Ar and N.Oc/B.Ar demonstrated significantly decreased number of osteoclasts in ONJ group versus non-ONJ group. CONCLUSIONS: These results show that serum TRACP 5b and the RANKL/OPG ratio were possible biomarkers for BRONJ. These data may provide useful additional information for future ONJ research. Further studies are needed to validate these results in humans with ONJ.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/cid.12297/abstract
DOI
10.1111/cid.12297
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
Yonsei Authors
Cha, In Ho(차인호) ORCID logo https://orcid.org/0000-0001-8259-2190
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146847
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