Cited 28 times in
Knockdown of apoptosis signal-regulating kinase 1 affects ischaemia-induced astrocyte activation and glial scar formation.
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2017-02-24T11:41:05Z | - |
dc.date.available | 2017-02-24T11:41:05Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0953-816X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/146833 | - |
dc.description.abstract | Reactive astrocytes play an essential role in determining the tissue response to ischaemia. Formation of a glial scar can block the neuronal outgrowth that is required for restoration of damaged tissue. Therefore, regulation of astrocyte activation is important; however, the mediator of this process has not been fully elucidated. Apoptosis signal-regulating kinase 1 (ASK1) is an early responder to oxidative stress, and plays a pivotal role in the intracellular signalling pathway of apoptosis, inflammation, and differentiation. To confirm whether ASK1 mediates astrocyte activation and leads to glial scar formation after cerebral ischaemia, we conducted in vivo and in vitro experiments. C57BL/6 mice were subjected to occlusion of the middle cerebral artery, and astrocyte cultures were exposed to oxygen-glucose deprivation. After silencing of ASK1 , astrocyte-associated genes were downregulated, as seen with the use of microarrays. The glial fibrillary acidic protein (GFAP) level was decreased, and correlated with the reduction in the ASK1 level. In astrocytes, reduction in the ASK1 level decreased the activity of the p38 pathway, and the levels of transcription factors for GFAP and GFAP transcripts after hypoxia. In the chronic phase, ASK1 depletion reduced glial scar formation and conserved neuronal structure, which may lead to better functional recovery. These data suggest that ASK1 may be an important mediator of ischaemia-induced astrocyte activation and scar formation, and could provide a potential therapeutic target for treatment after ischaemic stroke. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.format.extent | 912~922 | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.relation.isPartOf | EUROPEAN JOURNAL OF NEUROSCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Astrocytes/metabolism* | - |
dc.subject.MESH | Astrocytes/pathology | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cicatrix/metabolism* | - |
dc.subject.MESH | Glial Fibrillary Acidic Protein/genetics | - |
dc.subject.MESH | Glial Fibrillary Acidic Protein/metabolism | - |
dc.subject.MESH | Infarction, Middle Cerebral Artery/metabolism* | - |
dc.subject.MESH | Infarction, Middle Cerebral Artery/pathology | - |
dc.subject.MESH | MAP Kinase Kinase Kinase 5/genetics* | - |
dc.subject.MESH | MAP Kinase Kinase Kinase 5/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/metabolism | - |
dc.title | Knockdown of apoptosis signal-regulating kinase 1 affects ischaemia-induced astrocyte activation and glial scar formation. | - |
dc.type | Article | - |
dc.publisher.location | France | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Neurology | - |
dc.contributor.googleauthor | So Yeong Cheon | - |
dc.contributor.googleauthor | Kyoung Joo Cho | - |
dc.contributor.googleauthor | Juhyun Song | - |
dc.contributor.googleauthor | Gyung Whan Kim | - |
dc.identifier.doi | 10.1111/ejn.13175 | - |
dc.contributor.localId | A00310 | - |
dc.relation.journalcode | J00831 | - |
dc.identifier.eissn | 1460-9568 | - |
dc.identifier.pmid | 26797817 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/ejn.13175/abstract | - |
dc.subject.keyword | apoptosis signal-regulating kinase 1 | - |
dc.subject.keyword | cerebral ischaemia | - |
dc.subject.keyword | glial scar formation | - |
dc.subject.keyword | reactive astrocyte | - |
dc.subject.keyword | small interfering RNA | - |
dc.contributor.alternativeName | Kim, Gyung Whan | - |
dc.contributor.affiliatedAuthor | Kim, Gyung Whan | - |
dc.citation.volume | 43 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 912 | - |
dc.citation.endPage | 922 | - |
dc.identifier.bibliographicCitation | EUROPEAN JOURNAL OF NEUROSCIENCE, Vol.43(7) : 912-922, 2016 | - |
dc.date.modified | 2017-02-24 | - |
dc.identifier.rimsid | 47575 | - |
dc.type.rims | ART | - |
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