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Golgi polarization effects on infiltration of mesenchymal stem cells into electrospun scaffolds by fluid shear stress: Analysis by confocal microscopy and Fourier transform infrared spectroscopy

Authors
 Min Sung Kim  ;  Mi Hee Lee  ;  Byeong-Ju Kwon  ;  Min-Ah Koo  ;  Gyeung Mi Seon  ;  Jong Ho Lee  ;  Dong-Wook Han  ;  Jong-Chul Park 
Citation
 Applied Spectroscopy Reviews, Vol.51(7-9) : 570-581, 2016 
Journal Title
 Applied Spectroscopy Reviews 
ISSN
 0570-4928 
Issue Date
2016
Keywords
Golgi polarization ; cell infiltration ; mechanotaxis ; hMSCs ; FT-IR
Abstract
The polarization of the Golgi apparatus is an important phenomenon in the directional migration of many types of cells, including fluid shear stress enhanced infiltration of cells into scaffolds. Fourier transform infrared (FT-IR) spectroscopy would be a potential tool to study cell infiltration into scaffolds because this technique has simple, reproducible, non-destructive characteristics. Here, we investigated the effect of Golgi polarization on the directional migration and infiltration of human mesenchymal stem cells (hMSCs) into poly(lactic-co-glycolic acid) (PLGA) scaffolds by fluid shear stress. The cell infiltration into scaffolds by fluid shear stress was observed by immunofluorescence and FT-IR. 2 μM of Brefeldin A (BFA) inhibited the reorganization of Golgi polarization in hMSCs. The blocking of Golgi reorganization by BFA caused the suppression of directional migration and infiltration into PLGA scaffolds induced by 8 dyne/cm2 of fluid shear stress condition. In this study, we investigated how Golgi polarization plays an important role in the directional migration and infiltration of hMSCs into scaffolds by responding to the fluid shear stress. The possibility of FT-IR to be a potential tool for analysis of cell infiltration into scaffolds was identified since the immunofluorescence data matched FT-IR data.
Full Text
http://www.tandfonline.com/doi/abs/10.1080/05704928.2016.1166122
DOI
10.1080/05704928.2016.1166122
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
구민아(Koo, Min-Ah) ORCID logo https://orcid.org/0000-0002-8671-1131
권병주(Kwon, Byeong-Ju) ORCID logo https://orcid.org/0000-0001-9916-0546
박종철(Park, Jong Chul) ORCID logo https://orcid.org/0000-0003-0083-5991
선경미(Seon, Gyeung Mi) ORCID logo https://orcid.org/0000-0002-0766-1106
이미희(Lee, Mi Hee) ORCID logo https://orcid.org/0000-0002-9630-7044
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146734
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