0 341

Cited 1 times in

HIV migration between blood plasma and cellular subsets before and after HIV therapy

DC FieldValueLanguage
dc.contributor.author구남수-
dc.contributor.author김준명-
dc.contributor.author안미영-
dc.contributor.author안진영-
dc.contributor.author전용덕-
dc.contributor.author정인영-
dc.contributor.author최준용-
dc.date.accessioned2017-02-24T07:30:05Z-
dc.date.available2017-02-24T07:30:05Z-
dc.date.issued2016-
dc.identifier.issn0146-6615-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/146440-
dc.description.abstractThe cellular source of HIV RNA circulating in blood plasma remains unclear. Here, we investigated whether sequence analysis of HIV RNA populations circulating before combination antiretroviral therapy (cART) and HIV DNA populations in cellular subsets (CS) after cART could identify the cellular sources of circulating HIV RNA. Blood was collected from five subjects at cART initiation and again 6 months later. Naïve CD4+ T cells, resting central memory and effector memory CD4+ T cells, activated CD4+ T cells, monocytes, and natural killer cells were sorted using a fluorescence-activated cell sorter. HIV-1 env C2V3 sequences from HIV RNA in blood plasma and HIV DNA in CSs were generated using single genome sequencing. Sequences were evaluated for viral compartmentalization (Fst test) and migration events (MEs; Slatkin Maddison and cladistic measures) between blood plasma and each CS. Viral compartmentalization was observed in 88% of all cellular subset comparisons (range: 77-100% for each subject). Most observed MEs were directed from blood plasma to CSs (52 MEs, 85.2%). In particular, there was only viral movement from plasma to NK cells (15 MEs), monocytes (seven MEs), and naïve cells (five ME). We observed a total of nine MEs from activated CD4 cells (2/9 MEs), central memory T cells (3/9 MEs), and effector memory T cells (4/9 MEs) to blood plasma. Our results revealed that the HIV RNA population in blood plasma plays an important role in seeding various cellular reservoirs and that the cellular source of the HIV RNA population is activated central memory and effector memory T cells.-
dc.description.statementOfResponsibilityrestriction-
dc.format.extent606~613-
dc.languageEnglish-
dc.publisherWiley-Liss-
dc.relation.isPartOfJOURNAL OF MEDICAL VIROLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAnti-Retroviral Agents/therapeutic use-
dc.subject.MESHDNA, Viral/chemistry-
dc.subject.MESHDNA, Viral/genetics-
dc.subject.MESHHIV Infections/drug therapy-
dc.subject.MESHHIV Infections/virology*-
dc.subject.MESHHIV-1/classification-
dc.subject.MESHHIV-1/genetics-
dc.subject.MESHHIV-1/isolation & purification*-
dc.subject.MESHHumans-
dc.subject.MESHLeukocytes, Mononuclear/virology*-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHPlasma/virology*-
dc.subject.MESHRNA, Viral/blood*-
dc.subject.MESHRNA, Viral/genetics-
dc.subject.MESHSequence Analysis, DNA-
dc.subject.MESHenv Gene Products, Human Immunodeficiency Virus/genetics-
dc.titleHIV migration between blood plasma and cellular subsets before and after HIV therapy-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorJun Yong Choi-
dc.contributor.googleauthorAntoine Chai llon-
dc.contributor.googleauthorJin Ok Oh-
dc.contributor.googleauthorJin Young Ahn-
dc.contributor.googleauthorHae Won Ann-
dc.contributor.googleauthorIn Young Jung-
dc.contributor.googleauthorMi-Young Ahn-
dc.contributor.googleauthorYong Duk Jeon-
dc.contributor.googleauthorNam Su Ku-
dc.contributor.googleauthorDavey M. Smith-
dc.contributor.googleauthorJune Myung Kim-
dc.identifier.doi10.1002/jmv.24375-
dc.contributor.localIdA00189-
dc.contributor.localIdA00953-
dc.contributor.localIdA02224-
dc.contributor.localIdA02267-
dc.contributor.localIdA03534-
dc.contributor.localIdA03695-
dc.contributor.localIdA04191-
dc.relation.journalcodeJ01587-
dc.identifier.eissn1096-9071-
dc.identifier.pmid26348372-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jmv.24375/abstract-
dc.subject.keywordHIV-
dc.subject.keywordantiretroviral therapy-
dc.subject.keywordcladistics analysis-
dc.subject.keywordcompartmentalization-
dc.subject.keywordreservoir-
dc.subject.keywordviremia-
dc.contributor.alternativeNameKu, Nam Su-
dc.contributor.alternativeNameKim, June Myung-
dc.contributor.alternativeNameAhn, Mi Young-
dc.contributor.alternativeNameAhn, Jin Young-
dc.contributor.alternativeNameJeon, Yong Duk-
dc.contributor.alternativeNameJung, In Young-
dc.contributor.alternativeNameChoi, Jun Yong-
dc.contributor.affiliatedAuthorKu, Nam Su-
dc.contributor.affiliatedAuthorKim, June Myung-
dc.contributor.affiliatedAuthorAhn, Mi Young-
dc.contributor.affiliatedAuthorAhn, Jin Young-
dc.contributor.affiliatedAuthorJeon, Yong Duk-
dc.contributor.affiliatedAuthorJung, In Young-
dc.contributor.affiliatedAuthorChoi, Jun Yong-
dc.citation.volume88-
dc.citation.number4-
dc.citation.startPage606-
dc.citation.endPage613-
dc.identifier.bibliographicCitationJOURNAL OF MEDICAL VIROLOGY, Vol.88(4) : 606-613, 2016-
dc.date.modified2017-02-24-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.