Cited 49 times in
Flagellin suppresses experimental asthma by generating regulatory dendritic cells and T cells
DC Field | Value | Language |
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dc.contributor.author | 박중원 | - |
dc.date.accessioned | 2017-02-24T03:42:03Z | - |
dc.date.available | 2017-02-24T03:42:03Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0091-6749 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/146417 | - |
dc.description.abstract | BACKGROUND: Although the hygiene hypothesis suggests that microbial infections could subvert asthma and thus a microbial product might serve as a therapeutic adjuvant for asthma, the relationship between bacterial components and asthma is complex. Recently, low levels of flagellin, the Toll-like receptor (TLR) 5 ligand, have been reported to promote asthma. OBJECTIVE: We show that a therapeutic dose of flagellin suppresses asthma and that the effect occurs through generating regulatory dendritic cells (rDCs) and regulatory T (Treg) cells. METHODS: Ovalbumin (OVA)-induced wild-type and TLR5 knockout asthmatic mice were treated intranasally with a mixture of OVA and 10 μg of a flagellin B (FlaB; of Vibrio vulnificus). OVA/FlaB-treated rDCs were adoptively transferred to mice with OVA-induced asthma. Anti-CD25 mAb was used to deplete Treg cells. A mixture of house dust mite (HDM) and FlaB was used to treat mice with HDM-induced asthma. Blood CD14(+) monocyte-derived dendritic cells from HDM-sensitive asthmatic patients were treated with FlaB and incubated with autologous CD4(+) T cells. RESULTS: An OVA/FlaB mixture ameliorated OVA-induced asthma by inhibiting TH1/TH2/TH17 responses in a TLR5-dependent manner through generating rDCs and Treg cells. The adoptive transfer of OVA/FlaB-treated dendritic cells inhibited OVA-induced asthma, whereas the depletion of CD25(+) cells eliminated the inhibitory effect. A similar effect of FlaB was observed in mice with HDM-induced asthma. In patients with HDM-sensitive asthma, FlaB-treated rDCs inhibited HDM-stimulated TH1/TH2 responses while enhancing Treg cells in an IL-10-dependent manner. CONCLUSION: These findings collectively suggest that flagellin could be used as a tolerogenic adjuvant to treat allergic asthma. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.format.extent | 426~435 | - |
dc.language | English | - |
dc.publisher | St Louis, Mosby | - |
dc.relation.isPartOf | JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adoptive Transfer | - |
dc.subject.MESH | Allergens/immunology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Asthma/genetics | - |
dc.subject.MESH | Asthma/immunology* | - |
dc.subject.MESH | Asthma/metabolism* | - |
dc.subject.MESH | Asthma/pathology | - |
dc.subject.MESH | Asthma/therapy | - |
dc.subject.MESH | Case-Control Studies | - |
dc.subject.MESH | Dendritic Cells/immunology* | - |
dc.subject.MESH | Dendritic Cells/metabolism | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Flagellin/immunology* | - |
dc.subject.MESH | Immunomodulation* | - |
dc.subject.MESH | Ligands | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | Ovalbumin/immunology | - |
dc.subject.MESH | Pyroglyphidae/immunology | - |
dc.subject.MESH | T-Lymphocytes, Regulatory/immunology* | - |
dc.subject.MESH | T-Lymphocytes, Regulatory/metabolism | - |
dc.subject.MESH | Toll-Like Receptor 5/genetics | - |
dc.subject.MESH | Toll-Like Receptor 5/metabolism | - |
dc.title | Flagellin suppresses experimental asthma by generating regulatory dendritic cells and T cells | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Internal Medicine | - |
dc.contributor.googleauthor | Jae-Uoong Shim | - |
dc.contributor.googleauthor | Shee Eun Lee | - |
dc.contributor.googleauthor | Won Hwang | - |
dc.contributor.googleauthor | Changhon Lee | - |
dc.contributor.googleauthor | Jung-Won Park | - |
dc.contributor.googleauthor | Jung Ho Sohn | - |
dc.contributor.googleauthor | Jong Hee Nam | - |
dc.contributor.googleauthor | Young Kim | - |
dc.contributor.googleauthor | Joon Haeng Rhee | - |
dc.contributor.googleauthor | Sin-Hyeog Im | - |
dc.contributor.googleauthor | YoungIl Koh | - |
dc.identifier.doi | 10.1016/j.jaci.2015.07.010 | - |
dc.contributor.localId | A01681 | - |
dc.relation.journalcode | J01228 | - |
dc.identifier.eissn | 1097-6825 | - |
dc.identifier.pmid | 26303344 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0091674915009525 | - |
dc.subject.keyword | Asthma | - |
dc.subject.keyword | Toll-like receptor 5 | - |
dc.subject.keyword | dendritic cells | - |
dc.subject.keyword | flagellin | - |
dc.subject.keyword | regulatory T cells | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | Park, Jung Won | - |
dc.citation.volume | 137 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 426 | - |
dc.citation.endPage | 435 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Vol.137(2) : 426-435, 2016 | - |
dc.date.modified | 2017-02-24 | - |
dc.identifier.rimsid | 47925 | - |
dc.type.rims | ART | - |
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