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Real-time monitoring of 3D cell culture using a 3D capacitance biosensor

Authors
 Sun-Mi Lee  ;  Nalae Han  ;  Rimi Lee  ;  In-Hong Choi  ;  Yong-Beom Park  ;  Jeon-Soo Shin  ;  Kyung-Hwa Yoo 
Citation
 Biosensors & Bioelectronics, Vol.77 : 56-61, 2016 
Journal Title
 Biosensors & Bioelectronics 
ISSN
 0956-5663 
Issue Date
2016
MeSH
Biological Assay/instrumentation* ; Biosensing Techniques/instrumentation* ; Cell Culture Techniques/instrumentation* ; Cell Movement/physiology ; Cells, Cultured ; Computer Systems ; Conductometry/instrumentation* ; Electric Capacitance ; Equipment Design ; Equipment Failure Analysis ; Humans ; MCF-7 Cells ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/physiology* ; Monitoring, Physiologic/instrumentation*
Keywords
Apoptosis ; Capacitance sensor ; Migration ; Proliferation ; Real-time monitoring ; Three-dimensional (3D) cell culture
Abstract
Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor.
Full Text
http://www.sciencedirect.com/science/article/pii/S0956566315304024
DOI
10.1016/j.bios.2015.09.005
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실)
Yonsei Authors
박용범(Park, Yong Beom)
신전수(Shin, Jeon Soo) ORCID logo https://orcid.org/0000-0002-8294-3234
최인홍(Choi, In Hong) ORCID logo https://orcid.org/0000-0001-9851-0137
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146368
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