0 256

Cited 3 times in

Transgenic mouse models generated by hydrodynamic transfection for genetic studies of liver cancer and preclinical testing of anti-cancer therapy

Authors
 Hye-Lim Ju  ;  Kwang-Hyub Han  ;  Jong Doo Lee  ;  Simon Weonsang Ro 
Citation
 International Journal of Cancer, Vol.138(7) : 1601-1608, 2016 
Journal Title
 International Journal of Cancer 
ISSN
 0020-7136 
Issue Date
2016
MeSH
Animals ; Antineoplastic Agents/pharmacology ; Carcinoma, Hepatocellular/drug therapy ; Carcinoma, Hepatocellular/genetics* ; Hydrodynamics ; Liver Neoplasms, Experimental/drug therapy ; Liver Neoplasms, Experimental/genetics ; Mice ; Mice, Transgenic ; Molecular Targeted Therapy ; Transfection/methods*
Keywords
hepatocellular carcinoma ; hydrodynamics-based transfection ; oncogenic collaboration ; preclinical testing ; transgenic mouse model
Abstract
Hepatocellular carcinoma (HCC) is one of the most lethal cancers worldwide; however, the genetic mechanisms underlying its pathogenesis are incompletely understood. Genetically engineered mouse (GEM) models of HCC have been developed to elucidate the role of individual cancer-related genes in hepatocarcinogenesis. However, the expensive and time-consuming processes related to generating a GEM model discourage the development of diverse genotype models. Recently, a simple and inexpensive liver-specific transgenic approach was developed, in which a hydrodynamics-based transfection (HT) method was coupled with the Sleeping Beauty transposase system. Various HT models in which different oncogenic pathways are activated and/or tumor-suppressing pathways inactivated have been developed in recent years. The applicability of HT models in liver cancer research is expected to broaden and ultimately elucidate the cooperation between oncogenic signaling pathways and aid in designing molecular therapy to target altered pathways.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/ijc.29703/abstract
DOI
10.1002/ijc.29703
Appears in Collections:
5. Research Institutes (연구소) > Liver Cirrhosis Clinical Research Center (간경변증임상연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
노원상(Ro, Simon Weonsang) ORCID logo https://orcid.org/0000-0003-2187-3698
한광협(Han, Kwang-Hyub) ORCID logo https://orcid.org/0000-0003-3960-6539
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146364
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse