Cited 14 times in
Anti-Proliferative and Apoptotic Activities of M?llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines.
DC Field | Value | Language |
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dc.contributor.author | 서석교 | - |
dc.contributor.author | 김상운 | - |
dc.contributor.author | 김성훈 | - |
dc.contributor.author | 김영태 | - |
dc.contributor.author | 남은지 | - |
dc.contributor.author | 최영식 | - |
dc.date.accessioned | 2017-01-19T12:59:31Z | - |
dc.date.available | 2017-01-19T12:59:31Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/145515 | - |
dc.description.abstract | PURPOSE: This study aimed to investigate whether Müllerian inhibiting substance (MIS) in combination with calcitriol modulates proliferation and apoptosis of human ovarian cancer (OCa) cell lines (SKOV3, OVCAR3, and OVCA433) and identify the signaling pathway by which MIS mediates apoptosis. MATERIALS AND METHODS: OCa cell lines were treated with MIS in the absence or presence of calcitriol. Cell viability and proliferation were evaluated using the Cell Counting Kit-8 assay and apoptosis was evaluated by DNA fragmentation assay. Western blot and enzyme-linked immunosorbent assay were used to determine the signaling pathway. RESULTS: The cells showed specific staining for the MIS type II receptor. Treatment of OCa cells with MIS and calcitriol led to dose- and time-dependent inhibition of cell growth and survival. The combination treatment significantly suppressed cell growth, down-regulated the expression of B-cell lymphoma 2 (Bcl-2), and up-regulated the expressions of Bcl-2 associated X protein, caspase-3, and caspase-9 through the extracellular signal-regulated kinase signaling pathway. CONCLUSION: These results, coupled with a much-needed decrease in the toxic side effects of currently employed therapeutic agents, provide a strong rationale for testing the therapeutic potential of MIS, alone or in combination with calcitriol, in the treatment of OCa. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 33~40 | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.subject.MESH | Anti-Mullerian Hormone/pharmacology* | - |
dc.subject.MESH | Apoptosis/drug effects* | - |
dc.subject.MESH | Calcitriol/pharmacology* | - |
dc.subject.MESH | Caspase 3/metabolism | - |
dc.subject.MESH | Caspase 9/metabolism | - |
dc.subject.MESH | Cell Cycle/drug effects | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Proliferation/drug effects* | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | DNA Fragmentation/drug effects* | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/metabolism | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Growth Inhibitors/metabolism | - |
dc.subject.MESH | Growth Inhibitors/pharmacology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Ovarian Neoplasms/drug therapy* | - |
dc.subject.MESH | Ovarian Neoplasms/metabolism | - |
dc.subject.MESH | Ovarian Neoplasms/pathology* | - |
dc.subject.MESH | Receptors, Peptide | - |
dc.subject.MESH | Receptors, Transforming Growth Factor beta | - |
dc.subject.MESH | Signal Transduction/drug effects* | - |
dc.title | Anti-Proliferative and Apoptotic Activities of M?llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines. | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Obstetrics & Gynecology | - |
dc.contributor.googleauthor | Yeon Soo Jung | - |
dc.contributor.googleauthor | Hee Jung Kim | - |
dc.contributor.googleauthor | Seok Kyo Seo | - |
dc.contributor.googleauthor | Young Sik Choi | - |
dc.contributor.googleauthor | Eun Ji Nam | - |
dc.contributor.googleauthor | Sunghoon Kim | - |
dc.contributor.googleauthor | Sang Wun Kim | - |
dc.contributor.googleauthor | Hyuck Dong Han | - |
dc.contributor.googleauthor | Jae Wook Kim | - |
dc.contributor.googleauthor | Young Tae Kim | - |
dc.identifier.doi | 10.3349/ymj.2016.57.1.33 | - |
dc.contributor.localId | A01888 | - |
dc.contributor.localId | A00526 | - |
dc.contributor.localId | A00595 | - |
dc.contributor.localId | A00729 | - |
dc.contributor.localId | A01262 | - |
dc.contributor.localId | A04114 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 26632380 | - |
dc.subject.keyword | Müllerian inhibiting substance | - |
dc.subject.keyword | Ovarian cancer | - |
dc.subject.keyword | antiproliferation | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | calcitriol | - |
dc.contributor.alternativeName | Seo, Seok Kyo | - |
dc.contributor.alternativeName | Kim, Sang Wun | - |
dc.contributor.alternativeName | Kim, Sung Hoon | - |
dc.contributor.alternativeName | Kim, Young Tae | - |
dc.contributor.alternativeName | Nam, Eun Ji | - |
dc.contributor.alternativeName | Choi, Young Sik | - |
dc.contributor.affiliatedAuthor | Seo, Seok Kyo | - |
dc.contributor.affiliatedAuthor | Kim, Sang Wun | - |
dc.contributor.affiliatedAuthor | Kim, Sung Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Young Tae | - |
dc.contributor.affiliatedAuthor | Nam, Eun Ji | - |
dc.contributor.affiliatedAuthor | Choi, Young Sik | - |
dc.citation.volume | 57 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 33 | - |
dc.citation.endPage | 40 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.57(1) : 33-40, 2016 | - |
dc.date.modified | 2017-01-16 | - |
dc.identifier.rimsid | 47371 | - |
dc.type.rims | ART | - |
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