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Anti-Proliferative and Apoptotic Activities of M?llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines.

DC Field Value Language
dc.contributor.author서석교-
dc.contributor.author김상운-
dc.contributor.author김성훈-
dc.contributor.author김영태-
dc.contributor.author남은지-
dc.contributor.author최영식-
dc.date.accessioned2017-01-19T12:59:31Z-
dc.date.available2017-01-19T12:59:31Z-
dc.date.issued2016-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/145515-
dc.description.abstractPURPOSE: This study aimed to investigate whether Müllerian inhibiting substance (MIS) in combination with calcitriol modulates proliferation and apoptosis of human ovarian cancer (OCa) cell lines (SKOV3, OVCAR3, and OVCA433) and identify the signaling pathway by which MIS mediates apoptosis. MATERIALS AND METHODS: OCa cell lines were treated with MIS in the absence or presence of calcitriol. Cell viability and proliferation were evaluated using the Cell Counting Kit-8 assay and apoptosis was evaluated by DNA fragmentation assay. Western blot and enzyme-linked immunosorbent assay were used to determine the signaling pathway. RESULTS: The cells showed specific staining for the MIS type II receptor. Treatment of OCa cells with MIS and calcitriol led to dose- and time-dependent inhibition of cell growth and survival. The combination treatment significantly suppressed cell growth, down-regulated the expression of B-cell lymphoma 2 (Bcl-2), and up-regulated the expressions of Bcl-2 associated X protein, caspase-3, and caspase-9 through the extracellular signal-regulated kinase signaling pathway. CONCLUSION: These results, coupled with a much-needed decrease in the toxic side effects of currently employed therapeutic agents, provide a strong rationale for testing the therapeutic potential of MIS, alone or in combination with calcitriol, in the treatment of OCa.-
dc.description.statementOfResponsibilityopen-
dc.format.extent33~40-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.subject.MESHAnti-Mullerian Hormone/pharmacology*-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHCalcitriol/pharmacology*-
dc.subject.MESHCaspase 3/metabolism-
dc.subject.MESHCaspase 9/metabolism-
dc.subject.MESHCell Cycle/drug effects-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Proliferation/drug effects*-
dc.subject.MESHCell Survival/drug effects-
dc.subject.MESHDNA Fragmentation/drug effects*-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHExtracellular Signal-Regulated MAP Kinases/metabolism-
dc.subject.MESHFemale-
dc.subject.MESHGrowth Inhibitors/metabolism-
dc.subject.MESHGrowth Inhibitors/pharmacology-
dc.subject.MESHHumans-
dc.subject.MESHOvarian Neoplasms/drug therapy*-
dc.subject.MESHOvarian Neoplasms/metabolism-
dc.subject.MESHOvarian Neoplasms/pathology*-
dc.subject.MESHReceptors, Peptide-
dc.subject.MESHReceptors, Transforming Growth Factor beta-
dc.subject.MESHSignal Transduction/drug effects*-
dc.titleAnti-Proliferative and Apoptotic Activities of M?llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines.-
dc.typeArticle-
dc.publisher.locationKorea (South)-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Obstetrics & Gynecology-
dc.contributor.googleauthorYeon Soo Jung-
dc.contributor.googleauthorHee Jung Kim-
dc.contributor.googleauthorSeok Kyo Seo-
dc.contributor.googleauthorYoung Sik Choi-
dc.contributor.googleauthorEun Ji Nam-
dc.contributor.googleauthorSunghoon Kim-
dc.contributor.googleauthorSang Wun Kim-
dc.contributor.googleauthorHyuck Dong Han-
dc.contributor.googleauthorJae Wook Kim-
dc.contributor.googleauthorYoung Tae Kim-
dc.identifier.doi10.3349/ymj.2016.57.1.33-
dc.contributor.localIdA01888-
dc.contributor.localIdA00526-
dc.contributor.localIdA00595-
dc.contributor.localIdA00729-
dc.contributor.localIdA01262-
dc.contributor.localIdA04114-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid26632380-
dc.subject.keywordMüllerian inhibiting substance-
dc.subject.keywordOvarian cancer-
dc.subject.keywordantiproliferation-
dc.subject.keywordapoptosis-
dc.subject.keywordcalcitriol-
dc.contributor.alternativeNameSeo, Seok Kyo-
dc.contributor.alternativeNameKim, Sang Wun-
dc.contributor.alternativeNameKim, Sung Hoon-
dc.contributor.alternativeNameKim, Young Tae-
dc.contributor.alternativeNameNam, Eun Ji-
dc.contributor.alternativeNameChoi, Young Sik-
dc.contributor.affiliatedAuthorSeo, Seok Kyo-
dc.contributor.affiliatedAuthorKim, Sang Wun-
dc.contributor.affiliatedAuthorKim, Sung Hoon-
dc.contributor.affiliatedAuthorKim, Young Tae-
dc.contributor.affiliatedAuthorNam, Eun Ji-
dc.contributor.affiliatedAuthorChoi, Young Sik-
dc.citation.volume57-
dc.citation.number1-
dc.citation.startPage33-
dc.citation.endPage40-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.57(1) : 33-40, 2016-
dc.date.modified2017-01-16-
dc.identifier.rimsid47371-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers

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