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척추 황색 인대 세포에서 Bone Morphogenetic Protein-2 cDNA 전달에 의한 골형성 유도 : 조직 공학적 접근의 가능성
DC Field | Value | Language |
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dc.contributor.author | 김학선 | - |
dc.contributor.author | 문성환 | - |
dc.contributor.author | 원정훈 | - |
dc.contributor.author | 이환모 | - |
dc.contributor.author | 한수봉 | - |
dc.date.accessioned | 2016-05-16T11:30:47Z | - |
dc.date.available | 2016-05-16T11:30:47Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1229-5701 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144716 | - |
dc.description.abstract | Study Design In-vitro experiment. Objectives To determine the effect of bone morphogenetic protein-2 in the osteogenesis of human ligamentum flavum cells and test the feasibility of gene transfer to these cells. Summary of literature review Bone morphogenetic protein-2 (BMP-2) is known to be an important factor in the differentiation and maintenance of the osteoblastic phenotype. Tissue engineering for osteogenesis in ligamentum flavum by BMP-2 and gene transfer has not been previously studied. Materials and Methods Ligmentum flavum cells were harvested and cultured from surgical patients with spinal stenosis. BMP-2 was produced by transfecting pcDNA3.1/Hygro/BMP-2 into CHO cells using Lipofectamine 2000. Adenovirus-lacZ (Ad/lacZ) was also produced, and administered with BMP-2 to cell culture. The expression of lacZ was analyzed by X-gal staining. Bone formation was assessed by alkaline phosphatase, von Kossa, and alizarin Red-S staining, and the expression of osteocalcin was determined immunocytochemically. Results Ligamentum flavum cell cultures with Ad/lacZ showed marker gene expression. BMP-2 induced osteogenesis in ligamentum flavum cells as evidenced by alkaline phosphatase, von Kosa, and alizarin Red-S staining. Also, cell culture with BMP-2 showed strong positivity with osteocalcin by immunocytochemistry. Conclusion BMP-2 more strongly induced the osteogenesis of ligamentum flavum, and also its gene transfer to ligamentum flavum was found to be feasible. These results may open a new era of ligamentum flavum tissue engineering. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 263~269 | - |
dc.relation.isPartOf | Journal of Korean Society of Spine Surgery (대한척추외과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 척추 황색 인대 세포에서 Bone Morphogenetic Protein-2 cDNA 전달에 의한 골형성 유도 : 조직 공학적 접근의 가능성 | - |
dc.title.alternative | Osteogenesis by Transfer of Bone Morphogenetic Protein-2 cDNA in Ligamentum Flavum Cells : Approach Toward Tissue Engineering | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | 문성환 | - |
dc.contributor.googleauthor | 김향 | - |
dc.contributor.googleauthor | 권언혜 | - |
dc.contributor.googleauthor | 원정훈 | - |
dc.contributor.googleauthor | 김학선 | - |
dc.contributor.googleauthor | 한수봉 | - |
dc.contributor.googleauthor | 이환모 | - |
dc.identifier.doi | 10.4184/jkss.2002.9.4.263 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01093 | - |
dc.contributor.localId | A01365 | - |
dc.contributor.localId | A02440 | - |
dc.contributor.localId | A03333 | - |
dc.contributor.localId | A04293 | - |
dc.relation.journalcode | J01550 | - |
dc.subject.keyword | BMP-2 | - |
dc.subject.keyword | adenovirus | - |
dc.subject.keyword | osteogenesis | - |
dc.subject.keyword | Tissue engineering | - |
dc.contributor.alternativeName | Kim, Hak Sun | - |
dc.contributor.alternativeName | Moon, Seong Hwan | - |
dc.contributor.alternativeName | Won, Jung Hoon | - |
dc.contributor.alternativeName | Lee, Hwan Mo | - |
dc.contributor.alternativeName | Hahn, Soo Bong | - |
dc.contributor.affiliatedAuthor | Kim, Hak Sun | - |
dc.contributor.affiliatedAuthor | Moon, Seong Hwan | - |
dc.contributor.affiliatedAuthor | Won, Jung Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Hwan Mo | - |
dc.contributor.affiliatedAuthor | Hahn, Soo Bong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 9 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 263 | - |
dc.citation.endPage | 269 | - |
dc.identifier.bibliographicCitation | Journal of Korean Society of Spine Surgery (대한척추외과학회지), Vol.9(4) : 263-269, 2002 | - |
dc.identifier.rimsid | 51395 | - |
dc.type.rims | ART | - |
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