Cited 16 times in
Enhancement of metabolic oxidative stress-induced cytotoxicity by the thioredoxin inhibitor 1-methylpropyl 2-imidazolyl disulfide is mediated through the ASK1-SEK1-JNK1 pathway
DC Field | Value | Language |
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dc.contributor.author | 송재진 | - |
dc.date.accessioned | 2016-05-16T11:30:39Z | - |
dc.date.available | 2016-05-16T11:30:39Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0026-895X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144711 | - |
dc.description.abstract | We observed previously that glucose deprivation induces cytotoxicity, increases the intracellular levels of hydroperoxide, and activates the stress-activated protein kinase (SEK) pathway. In this study, we hypothesized that 1-methylpropyl 2-imidazolyl disulfide (IV-2), a thioredoxin (TRX) inhibitor, augments glucose deprivation-induced cytotoxicity by promoting c-Jun N-terminal kinase (JNK) activation. Human prostatic carcinoma DU-145 cells were exposed to glucose-free medium containing various concentrations of IV-2 (10–50 μM). Glucose deprivation alone or IV-2 alone induced minimal cytotoxicity within 7 h. However, the combination of glucose deprivation and IV-2 increased cell death in a dose-dependent manner. The cytotoxicity was suppressed by treatment with an antioxidant,N-acetyl-l-cysteine or overexpressing TRX. The combined glucose deprivation and IV-2 treatment also promoted glucose deprivation-induced JNK1 activation by disrupting the interaction between TRX and apoptosis signal-regulating kinase 1 (ASK1). Overexpression of the JNK1 dominant-negative mutant inhibited the activation of the SEK pathway and protected cells from glucose deprivation and IV-2–induced cytotoxicity. Therefore, IV-2 enhances glucose deprivation-induced cytotoxicity by promoting glucose deprivation-induced activation of the ASK1-SEK1-JNK1 pathway. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1409~1417 | - |
dc.relation.isPartOf | MOLECULAR PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Enhancement of metabolic oxidative stress-induced cytotoxicity by the thioredoxin inhibitor 1-methylpropyl 2-imidazolyl disulfide is mediated through the ASK1-SEK1-JNK1 pathway | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Life Science (의생명과학부) | - |
dc.contributor.googleauthor | Yong J. Lee | - |
dc.contributor.googleauthor | Jin H. Kim | - |
dc.contributor.googleauthor | Jun Chen | - |
dc.contributor.googleauthor | Jae J. Song | - |
dc.identifier.doi | 10.1124/mol.62.6.1409 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02056 | - |
dc.relation.journalcode | J02267 | - |
dc.identifier.eissn | 1521-0111 | - |
dc.contributor.alternativeName | Song, Jae Jin | - |
dc.contributor.affiliatedAuthor | Song, Jae Jin | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 62 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1409 | - |
dc.citation.endPage | 1417 | - |
dc.identifier.bibliographicCitation | MOLECULAR PHARMACOLOGY, Vol.62(6) : 1409-1417, 2002 | - |
dc.identifier.rimsid | 51390 | - |
dc.type.rims | ART | - |
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