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새우젓(salted and fermented shrimp)의 항원성 변화에 관한 연구
DC Field | Value | Language |
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dc.contributor.author | 강은석 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2016-05-16T11:28:37Z | - |
dc.date.available | 2016-05-16T11:28:37Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1226-8739 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144635 | - |
dc.description.abstract | BACKGROUND AND OBJECTIVE: Shrimp is one of the most common food allergens. The salted and fermented shrimp (S/F shrimp) is a traditional food and ingested frequently in the daily life of many Korean people. But few studies have been investigated on the allergenicity of S/F shrimp. The aim of our study is to observe the allergenicity of S/F shrimp and to compare it with the allergenicity of shrimp MATERIAL AND METHODS: Crude extracts were made from three kinds of S/F shrimps and four kinds of shrimps after boiling. The extracts were used for SDS-PAGE, ELISA, ELISA inhibition test, immunoblotting, and immunoblotting inhibition test with sera from 10 subjects who have shrimp specific IgE by radioallergosorbent (RAST) (3+ or 4+). RESULTS: The protein concentrations of S/F shrimp extracts were less than half of the extracts of shrimps (1.9 mg/ml, 1.2 mg/ml, 1.1 mg/ml vs 4mg/ml, 3.83 mg/ml, 6.8 mg/ml, 6.6 mg/ml). The extracts of S/F shrimp showed weaker and more diffusely distributed protein bands in SDS-PAGE and showed less than half of the O.D. value in IgE-ELISA compared to those of shrimp extracts. The specific IgE immunoblotting of S/F shrimp extracts showed 9 bands (36, 33, 30, 29, 28, 26, 23, 20, 19 kDa). Dose dependent inhibition was observed between shrimps and S/F shrimp in ELISA-inhibition and immnunoblotting inhibition test. The allergen concentration of S/F shrimp needed to inhibit 50% of shrimp-lgE ELISA was five times higher than that of shrimp. CONCLUSION: The crude extracts of S/F shrimps showed less than half the amount of protein compared to crude extracts of shrimps have and also showed reduced allergenicity by salting and fermentation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | Journal of Asthma, Allergy and Clinical Immunology (천식 및 알레르기) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | salted and fermented shrimps | - |
dc.subject.MESH | shrimps | - |
dc.subject.MESH | allergenecity | - |
dc.title | 새우젓(salted and fermented shrimp)의 항원성 변화에 관한 연구 | - |
dc.title.alternative | Changes of allergenecity of salted and fermented shrimp | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | 배상운 | - |
dc.contributor.googleauthor | 최수영 | - |
dc.contributor.googleauthor | 진현선 | - |
dc.contributor.googleauthor | 김철우 | - |
dc.contributor.googleauthor | 이광은 | - |
dc.contributor.googleauthor | 강은석 | - |
dc.contributor.googleauthor | 박준용 | - |
dc.contributor.googleauthor | 홍성필 | - |
dc.contributor.googleauthor | 최훈영 | - |
dc.contributor.googleauthor | 정재헌 | - |
dc.contributor.googleauthor | 김용수 | - |
dc.contributor.googleauthor | 홍천수 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00068 | - |
dc.contributor.localId | A04448-1 | - |
dc.relation.journalcode | J01251 | - |
dc.subject.keyword | salted and fermented shrimps | - |
dc.subject.keyword | shrimps | - |
dc.subject.keyword | allergenecity | - |
dc.contributor.alternativeName | Kang, Eun Seok | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Kang, Eun Seok | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 23 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 44 | - |
dc.citation.endPage | 52 | - |
dc.identifier.bibliographicCitation | Journal of Asthma, Allergy and Clinical Immunology (천식 및 알레르기), Vol.23(1) : 44-52, 2003 | - |
dc.identifier.rimsid | 51320 | - |
dc.type.rims | ART | - |
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