정상인과 아토피피부염 환자 혈청의 프로티옴 지도 비교

Abstract

Atopic dermatitis is the most common chronic inflammatory skin disease that occurs in children and sustains to adults with increasing incidence. However, we have diagnostic and therapeutic difficulties because of the wide range in symptomatology and the fluctuating course as well as unknown pathophysiology. Proteomics, based on the protein measurement by two-dimensional gel electrophoresis followed by the protein identification and characterization by mass spectrometry, is a new emerging field for understanding cell physiology and pathophysiology of diseases. We acquired the proteome maps of the sera from atopic dermatitis patients and normal controls by isoelectric focusing in 3-10 non-linear immobilzed pH gradients strips and two-dimensional electrophoresis, and compared them. We also compared the patients´ sera according to the symptom severity and the disease activities. Quantitative differences in protein expressions between gel groups could be found and many increased or decreased spots due to protein changes were found to be statistically significant. We detected that 7 spots were changed in the sera of patients with atopic dermatitis in comparing with the sera of normal controls. Compared to the patients with mild symptom of atopic dermatitis, the patients with severe symptom showed 5 protein spots decreased. And 4 protein spots were increased in the sera of inactive disease status in comparing with the sera of active status. The changes in many protein spots were statistically significant in the comparison of the sera of patients with atopic dermatitis and normal controls. Proteomics in atopic dermatitis would be helpful in diagnosing the disease and evaluating the disease activity.