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cDNA microarray를 이용한 HPV-16 E6와 연관된 유전자의 발현 분석
DC Field | Value | Language |
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dc.contributor.author | 라선영 | - |
dc.date.accessioned | 2016-05-16T11:24:18Z | - |
dc.date.available | 2016-05-16T11:24:18Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0494-4755 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144475 | - |
dc.description.abstract | Objective: To examine the effect of HPV-16 E6 expression on the transcription of cellular genes, we used cDNA microarray in HPV-16 E6 transfected stable cancer cell lines. Methods: Using cDNA microarray consisting of 1,024 genes, we have performed a systematic characterization of gene expression in A549E6 human lung adenocarcinoma and RC10.1 human colon adenocarcinoma cell lines stably expressing HPV-16 E6 gene. The up-regulated and down-regulated genes were classified into the different functional categories; oncogenes, apoptosis, cell cycle, signal transduction, gene regulation, immune response, cell adhesion, protein transport, metabolism, redox control and angiogenesis. Results: Among 1,024 known genes and ESTs (expressed sequence tags) tested, we found 27 up- regulated and 43 down-regulated genes in A549E6 (HPV-16 E6) compared to A549. The major up-regulated genes were as follows. GTPase-activating protein Rho 4, transcription factor D2, IKAROS, integrin-α 6, cadherin 11, ephrin-β 2, RAN binding protein 2, branched-chain amino transferase 2. The major down-regulated genes were as follows. K-ras 2, CDC (cell division cycle) 37, CDC16, CDC7L1, IRF3, interferon-γ-inducible protein 30, cadherin 6, desmoglein 1, desmocollin 2, endothelin 2. Also, we found 48 up-regulated and 34 down-regulated genes in RC10.1 (HPV-16 E6) compared to RKO. The major up-regulated genes were as follows. Colon cancer familial nonpolyposis type 1 (COCA 1), Bcl 2, jagged 1, MAP2K6, E2F1, ephrin receptor-β 2, ephrin-β 2, desmoglein 1, transforming growth factor-β 3. The major down-regulated genes were as follows. KIT, Rad51C, Bcl 2 antagonist killer 1, STAT 4, epidermal growth factor receptor, high mobility group protein 2, cadherin 11, cadherin 12, cadherin 3, integrin-α 1, intergrin-α 8, chromosome segregation 1-like. Conclusion: Various expression patterns of cellular genes by HPV-16 E6 could be wholy grasped and classified into different functional groups using both cell line system stably expressed HPV-16 E6 and cDNA microarray analysis. These analysis methods must be helpful to understand multiple effects of a specific gene on cellular genes in a short period. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2280~2288 | - |
dc.relation.isPartOf | Korean Journal of Obstetrics and Gynecology (대한산부인과학회잡지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | cDNA microarray를 이용한 HPV-16 E6와 연관된 유전자의 발현 분석 | - |
dc.title.alternative | Analysis of HPV-16 E6-related gene expression using cDNA microarray | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | 명진 | - |
dc.contributor.googleauthor | 라선영 | - |
dc.contributor.googleauthor | 이명진 | - |
dc.contributor.googleauthor | 엄수종 | - |
dc.contributor.googleauthor | 남궁성은 | - |
dc.contributor.googleauthor | 박종섭 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01316 | - |
dc.relation.journalcode | J02075 | - |
dc.subject.keyword | Human papillomavirus | - |
dc.subject.keyword | cDNA microarray | - |
dc.subject.keyword | Cellular genes, | - |
dc.subject.keyword | HPV-16 E6 | - |
dc.contributor.alternativeName | Rha, Sun Young | - |
dc.contributor.affiliatedAuthor | Rha, Sun Young | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 45 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2280 | - |
dc.citation.endPage | 2288 | - |
dc.identifier.bibliographicCitation | Korean Journal of Obstetrics and Gynecology (대한산부인과학회잡지), Vol.45(12) : 2280-2288, 2002 | - |
dc.identifier.rimsid | 49740 | - |
dc.type.rims | ART | - |
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