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Bacterial DNA in mixed cholesterol gallstones

DC FieldValueLanguage
dc.contributor.author이동기-
dc.date.accessioned2016-05-16T11:24:16Z-
dc.date.available2016-05-16T11:24:16Z-
dc.date.issued1999-
dc.identifier.issn0002-9270-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/144474-
dc.description.abstractOBJECTIVE: Numerous investigators have proposed a role for bacteria in biliary lithogenesis. We hypothesized that bacterial DNA is present in gallstones, and that categorical differences exist between gallstone type and the frequency of bacterial sequences. METHODS: Polymerase chain reaction (PCR) was used to amplify bacterial 16S rRNA and uidA (encoding Escherichia coli [E. coli]beta-glucuronidase) genes in different types of gallstones. PCR products were sequenced. RESULTS: Bacterial 16S rRNA and uidA DNA sequences in E. coli were detected in all brown pigment, common bile duct, and mixed cholesterol gallstones (n = 14). In contrast, only one (14%) of seven pure cholesterol gallstones yielded a PCR product. Most (88%) mixed cholesterol gallstones yielded PCR amplification products from their central, as well as their outer, portions. Sequenced products possessed 88–98% identity to 16S rRNA genes of E. coli and Pseudomonas species. CONCLUSIONS: Bacterial DNA sequences are usually present in mixed cholesterol (to 95% cholesterol content), brown pigment, and common bile duct, but rarely in pure cholesterol gallstones. The presence of bacterial beta-glucuronidase is also suggested. The role of bacteria and their products in the formation of mixed cholesterol gallstones, which comprise the majority of cholesterol gallstones, warrants further study.-
dc.description.statementOfResponsibilityopen-
dc.format.extent3502~3506-
dc.relation.isPartOfAMERICAN JOURNAL OF GASTROENTEROLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleBacterial DNA in mixed cholesterol gallstones-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorDong Ki Lee-
dc.contributor.googleauthorPhillip I Tarr-
dc.contributor.googleauthorW Geoffrey Haigh-
dc.contributor.googleauthorSum P Lee-
dc.identifier.doi10.1111/j.1572-0241.1999.01614.x-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02723-
dc.relation.journalcodeJ00081-
dc.identifier.eissn1572-0241-
dc.identifier.urlhttp://www.nature.com/ajg/journal/v94/n12/full/ajg1999817a.html-
dc.contributor.alternativeNameLee, Dong Ki-
dc.contributor.affiliatedAuthorLee, Dong Ki-
dc.rights.accessRightsnot free-
dc.citation.volume94-
dc.citation.number12-
dc.citation.startPage3502-
dc.citation.endPage3506-
dc.identifier.bibliographicCitationAMERICAN JOURNAL OF GASTROENTEROLOGY, Vol.94(12) : 3502-3506, 1999-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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