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방사선에 의한 Apoptosis에서 Fas의 역할
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 성진실 | - |
dc.date.accessioned | 2016-05-16T11:22:14Z | - |
dc.date.available | 2016-05-16T11:22:14Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1229-8719 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144400 | - |
dc.description.abstract | Purpose: It has been recognized that interaction of the Fas : Fas ligand plays an important role in radiation-induced apoptosis. The purpose of this study was to investigate the role of Fas mutation in radiation-induced apoptosis in vivo. Materials and Methods: Mice with mutations in Fas, MRL/Mpj-Faslpr, and its normal control, MRL/Mpj, were used in this study. Eight-week old male mice were given whole body radiation. After irradiation, the mice were killed and their spleens were collected at different time intervals. Tissue samples were stained with hematoxylin-eosin and the numbers of apoptotic cells were scored. Regulating molecules of apoptosis including p53, Bcl-2, Bax, Bcl-XL, and Bcl-XS were also analyzed by Western blotting. Results: At 25 ㏉ irradiation, the level of apoptosis reached the peak value at 8 hr after radiation and recovered to the normal value at 24 hr after radiation in MRL/Mpj mice. In contrast, the peak apoptosis level appeared at 4 hr after radiation in MRL/Mpj-Faslpr mice. At 8 hr after radiation, the levels of apoptosis in MRL/Mpj mice and MRL/Mpj-Faslpr mice were 52.3±7.8% and 8.0±8.6%, respectively (p<0.05). The expression of apoptosis regulating molecules, p53, Bcl-XL and Bcl-XS, increased in MRL/Mpj mice in response to radiation; p53 with a peak level of 3-fold at 8 h, Bcl-XL with a peak level of 3.3-fold at 12 h, and Bcl-XS with a peak level of 3-fold at 12h after 25 ㏉ radiation. Bcl-2 and Bax did not show significant change in MRL/Mpj mice. However in MRL/Mpj-Faslpr mice, the expression levels of p53, Bcl-2, Bax, Bcl-XL and Bcl-XS showed no significant change. Conclusion: The level of radiation-induced apoptosis was lower in Fas mutated mice, lpr, than in control mice. This seemed to be related to the lack of radiation-induced p53 activation in the lpr mice. This result suggests that Fas plays an important role in radiation-induced apoptosis in vivo. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 246~252 | - |
dc.language | Korean | - |
dc.publisher | 대한방사선종양학회 | - |
dc.relation.isPartOf | Journal of the Korean Society for Therapeutic Radiology and Oncology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 방사선에 의한 Apoptosis에서 Fas의 역할 | - |
dc.title.alternative | The Role of Fas in Radiation Induced Apoptosis in vivo | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | 김성희 | - |
dc.contributor.googleauthor | 성진실 | - |
dc.contributor.googleauthor | 성제경 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01956 | - |
dc.relation.journalcode | J01857 | - |
dc.subject.keyword | 방사선 | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Fas | - |
dc.subject.keyword | Ipr | - |
dc.contributor.alternativeName | Seong, Jin Sil | - |
dc.contributor.affiliatedAuthor | Seong, Jin Sil | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 20 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 246 | - |
dc.citation.endPage | 252 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Society for Therapeutic Radiology and Oncology, Vol.20(3) : 246-252, 2002 | - |
dc.identifier.rimsid | 49680 | - |
dc.type.rims | ART | - |
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