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Role of protein kinase C- or RhoA-induced Ca2+-sensitization in stretch-induced myogenic tone

DC FieldValueLanguage
dc.contributor.author안덕선-
dc.contributor.author이영호-
dc.date.accessioned2016-05-16T11:21:04Z-
dc.date.available2016-05-16T11:21:04Z-
dc.date.issued2002-
dc.identifier.issn0008-6363-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/144358-
dc.description.abstractObjective: It has been suggested that Ca2+ sensitization mechanisms might contribute to myogenic tone. However, specific mechanisms have yet to be fully identified. Therefore, we investigated the role of protein kinase C (PKC)- or RhoA-induced Ca2+ sensitization in myogenic tone of the rabbit basilar vessel. Methods: Myogenic tone was developed by stretch of rabbit basilar artery. Fura-2 Ca2+ signals, contractile responses, PKC immunoblots, translocation of PKC and RhoA, and phosphorylation of myosin light chains were measured. Results: Stretch of the resting vessel evoked a myogenic contraction and an increase in the intracellular Ca2+ concentration ([Ca2+]i) only in the presence of extracellular Ca2+. Stretch evoked greater contraction than high K+ at a given [Ca2+]i. The stretch-induced increase in [Ca2+]i and contractile force were inhibited by treatment of the tissue with nifedipine, a blocker of voltage-dependent Ca2+ channel, but not with gadolinium, a blocker of stretch-activated cation channels. The PKC inhibitors, H-7 and calphostin C, and a RhoA-activated protein kinase (ROK) inhibitor, Y-27632, inhibited the stretch-induced myogenic tone without changing [Ca2+]i. Immunoblotting using isoform-specific antibodies showed the presence of PKCα and PKCε in the rabbit basilar artery. PKCα, but not PKCε, and RhoA were translocated from the cytosol to the cell membrane by stretch. Phosphorylation of the myosin light chains was increased by stretch and the increased phosphorylation was blocked by treatment of the tissue with H-7 and Y-27632, respectively. Conclusions: Our results are consistent with important roles for PKC and RhoA in the generation of myogenic tone. Furthermore, enhanced phosphorylation of the myosin light chains by activation of PKCα and/or RhoA may be key mechanisms for the Ca2+ sensitization associated myogenic tone in basilar vessels.-
dc.description.statementOfResponsibilityopen-
dc.format.extent431~438-
dc.relation.isPartOfCARDIOVASCULAR RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleRole of protein kinase C- or RhoA-induced Ca2+-sensitization in stretch-induced myogenic tone-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Physiology (생리학)-
dc.contributor.googleauthorDong Soo Yeon-
dc.contributor.googleauthorJung Sup Kim-
dc.contributor.googleauthorDuck Sun Ahn-
dc.contributor.googleauthorSeong Chun Kwon-
dc.contributor.googleauthorBok Soon Kang-
dc.contributor.googleauthorKathleen G. Morgan-
dc.contributor.googleauthorYoung Ho Lee-
dc.identifier.doi10.1016/S0008-6363(01)00496-5-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02223-
dc.contributor.localIdA02968-
dc.relation.journalcodeJ00464-
dc.identifier.eissn1755-3245-
dc.subject.keywordMicrocirculation-
dc.subject.keywordArteries-
dc.subject.keywordCalcium (cellular)-
dc.subject.keywordE–c coupling-
dc.subject.keywordStretch/m–e coupling-
dc.contributor.alternativeNameAhn, Duk Sun-
dc.contributor.alternativeNameLee, Young Ho-
dc.contributor.affiliatedAuthorAhn, Duk Sun-
dc.contributor.affiliatedAuthorLee, Young Ho-
dc.rights.accessRightsfree-
dc.citation.volume53-
dc.citation.number2-
dc.citation.startPage431-
dc.citation.endPage438-
dc.identifier.bibliographicCitationCARDIOVASCULAR RESEARCH, Vol.53(2) : 431-438, 2002-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers

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