Cited 21 times in
Enzyme-linked immunosorbent assay for the determination of 20(S)-protopanaxatriol
DC Field | Value | Language |
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dc.contributor.author | 정다운 | - |
dc.date.accessioned | 2016-05-16T11:20:44Z | - |
dc.date.available | 2016-05-16T11:20:44Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144345 | - |
dc.description.abstract | A highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) method has been developed for the determination of 20(S)-protopanaxatriol (PPT), one of the major aglycones of dammarane-type ginseng saponins. Polyclonal antibodies raised against ginsenoside F1 (GF1)-bovine serum albumin showed high reactivities to PPT and GF1, whereas they exhibited minor or even no cross-reactivities to other ginsenosides and protopanaxadiol (0.19%). The working range of this method extends from 50 pg ml−1 to 20 ng ml−1 of PPT. The assay reported here has been validated against an HPLC technique using PPT-containing samples and was shown to correlate closely (γ=0.993). This ELISA could be a useful tool for the determination of PPT contained in biological fluids and plant materials. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 157~163 | - |
dc.relation.isPartOf | ANALYTICA CHIMICA ACTA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Enzyme-linked immunosorbent assay for the determination of 20(S)-protopanaxatriol | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Oral Cancer Research Institute (구강종양연구소) | - |
dc.contributor.googleauthor | Da Woon Jung | - |
dc.contributor.googleauthor | Jeong Mi Lee | - |
dc.contributor.googleauthor | Chung Ki Sung | - |
dc.identifier.doi | 10.1016/S0003-2670(02)00340-9 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03588 | - |
dc.relation.journalcode | J00131 | - |
dc.identifier.eissn | 1873-4324 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0003267002003409 | - |
dc.subject.keyword | ELISA | - |
dc.subject.keyword | 20(S)-Protopanaxatriol | - |
dc.subject.keyword | Polyclonal antibodies | - |
dc.subject.keyword | Ginsenoside F1 | - |
dc.contributor.alternativeName | Jung, Da Woon | - |
dc.contributor.affiliatedAuthor | Jung, Da Woon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 462 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 157 | - |
dc.citation.endPage | 163 | - |
dc.identifier.bibliographicCitation | ANALYTICA CHIMICA ACTA, Vol.462(2) : 157-163, 2002 | - |
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