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Downregulation of ERK2 is essential for the inhibition of radiation-induced cell death in HSP25 overexpressed L929 cells.
DC Field | Value | Language |
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dc.contributor.author | 이윤실 | - |
dc.date.accessioned | 2016-05-16T11:19:59Z | - |
dc.date.available | 2016-05-16T11:19:59Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1350-9047 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144317 | - |
dc.description.abstract | We previously reported that overexpression of HSP25 delayed cell growth, increased the level of p21(waf), reduced the levels of cyclin D1, cyclin A and cdc2, and induced radioresistance in L929 cells. In this study, we demonstrated that HSP25 induced-radioresistance was abolished by transfection with plasmids containing antisense hsp25 cDNA. Extracellular regulated kinase (ERK) and MAP kinase/ERK kinase (MEK) expressions as well as their activation (phospho-forms) were inhibited by hsp25 overexpression. Furthermore, when control vector transfected cells were treated with PD98059, MEK inhibitor, they became resistant to radiation, suggesting that inhibition of ERK1/2 activities was essential for radioresistance in L929 cells. To confirm the relationship between ERK1/2 and hsp25-mediated radioresistance, ERK1 or ERK2 cDNA was transiently transfected into the hsp25 overexpressed cells and their radioresistance was examined. HSP25-mediated radioresistance was abolished by overexpression of ERK2, but not by overexpression of ERK1. Alteration of cell cycle distribution and cell cycle related protein expressions (cyclin D, cyclin A and cdc2) by hsp25 overexpression were also recovered by ERK2 cDNA transfection. Increase in Bcl-2 protein by hsp25 gene transfection was also reduced by subsequent ERK2 cDNA-transfection. Taken together, these results suggest that downregulation of ERK2 is essential for the inhibition of radiation-induced cell death in HSP25 overexpressed cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 448~456 | - |
dc.relation.isPartOf | CELL DEATH AND DIFFERENTIATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis*/radiation effects | - |
dc.subject.MESH | CellLine | - |
dc.subject.MESH | Down-Regulation* | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology | - |
dc.subject.MESH | Flavonoids/pharmacology | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Heat-Shock Proteins* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1/antagonists & inhibitors | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1/metabolism* | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3 | - |
dc.subject.MESH | Mitogen-Activated Protein Kinases/antagonists & inhibitors | - |
dc.subject.MESH | Mitogen-Activated Protein Kinases/metabolism | - |
dc.subject.MESH | Neoplasm Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Neoplasm Proteins/genetics | - |
dc.subject.MESH | Neoplasm Proteins/metabolism* | - |
dc.subject.MESH | Radiation Tolerance | - |
dc.subject.MESH | Transfection | - |
dc.title | Downregulation of ERK2 is essential for the inhibition of radiation-induced cell death in HSP25 overexpressed L929 cells. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | H N Cho | - |
dc.contributor.googleauthor | Y J Lee | - |
dc.contributor.googleauthor | C K Cho | - |
dc.contributor.googleauthor | S J Lee | - |
dc.contributor.googleauthor | Y-S Lee | - |
dc.identifier.doi | 10.1038/sj/cdd/4400979 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03021 | - |
dc.relation.journalcode | J00483 | - |
dc.identifier.eissn | 1476-5403 | - |
dc.identifier.pmid | 11965498 | - |
dc.subject.keyword | HSP25 | - |
dc.subject.keyword | Inhibition of cell death | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | cell growth | - |
dc.subject.keyword | ERK2 | - |
dc.contributor.alternativeName | Lee, Yun Sil | - |
dc.contributor.affiliatedAuthor | Lee, Yun Sil | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 9 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 448 | - |
dc.citation.endPage | 456 | - |
dc.identifier.bibliographicCitation | CELL DEATH AND DIFFERENTIATION, Vol.9(4) : 448-456, 2002 | - |
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