Cited 42 times in
Effects of charge destiny and particle size of poly(styrene/(dimethylamino)ethyl methacrylate) nanoparticle for gene delivery in 293 cells. Colloids and Surfaces B
DC Field | Value | Language |
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dc.contributor.author | 장양수 | - |
dc.date.accessioned | 2016-05-16T11:14:04Z | - |
dc.date.available | 2016-05-16T11:14:04Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144092 | - |
dc.description.abstract | To investigate factors for enhancing transfection efficiency, PS and positively charged PS nanoparticles were used as a model gene carrier system. Polymeric nanoparticles were synthesized using a simple and reproducible method of surfactant-free emulsion polymerization and charge densities of nanoparticles controlled by a two-stage shot-growth method. The morphology, charge density, and ξ-potential values of model nanoparticles were measured by SEM, the conductive method, and Zeta Plus, respectively. Four nanoparticles with different surface charge densities were adapted to investigate the capacity of condensing the gene and the enhancement of the transfection efficiency to the target cell. The cell viabilities in the presence of nanoparticles ranged between 75–110% of the control in all experiments. The highly charged nanoparticles (0.01 μC cm−2) fulfilled the requirements for a suitable model gene delivery system with respect to the condensing ability of DNA, complex formation, and transfection efficiency. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 213~222 | - |
dc.relation.isPartOf | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Effects of charge destiny and particle size of poly(styrene/(dimethylamino)ethyl methacrylate) nanoparticle for gene delivery in 293 cells. Colloids and Surfaces B | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Shi Won Pang | - |
dc.contributor.googleauthor | Hyun Young Park | - |
dc.contributor.googleauthor | Yang Soo Jang | - |
dc.contributor.googleauthor | Woo Sik Kim | - |
dc.contributor.googleauthor | Jung Hyun Kim | - |
dc.identifier.doi | 10.1016/S0927-7765(01)00335-6 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03448 | - |
dc.relation.journalcode | J00627 | - |
dc.identifier.eissn | 1873-4367 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0927776501003356 | - |
dc.subject.keyword | Non-viral | - |
dc.subject.keyword | Nanoparticle | - |
dc.subject.keyword | Gene transfer | - |
dc.subject.keyword | Polystyrene | - |
dc.subject.keyword | Dimethylaminoethyl methacrylate | - |
dc.contributor.alternativeName | Jang, Yang Soo | - |
dc.contributor.affiliatedAuthor | Jang, Yang Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 26 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 213 | - |
dc.citation.endPage | 222 | - |
dc.identifier.bibliographicCitation | COLLOIDS AND SURFACES B-BIOINTERFACES, Vol.26(3) : 213-222, 2002 | - |
dc.identifier.rimsid | 55579 | - |
dc.type.rims | ART | - |
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