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Focal adhesion kinase 발현 조절을 통한 연골육종 세포주의 침습성 조절
DC Field | Value | Language |
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dc.contributor.author | 신규호 | - |
dc.contributor.author | 이진우 | - |
dc.contributor.author | 한수봉 | - |
dc.date.accessioned | 2016-05-16T11:13:32Z | - |
dc.date.available | 2016-05-16T11:13:32Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1226-2102 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144072 | - |
dc.description.abstract | Purpose : We propose that cell attachment and invasion can be regulated by the modulation of FAK expression in chondrosarcoma cell lines. Materials and Methods : The C-terminal domain of FAK (FAK-CD) was transfected by recombinant adenovirus infection in chondrosarcoma cell lines, JJ012 and 105KC. The expression of FAK, FAK-CD and tyrosine phosphorylation were checked. Chondrocytes and chondrosarcoma cells were used in cell attachment tests by blocking or not blocking integrin- 1 antibodies and synthetic peptides on type II collagen. To evaluate the effect of cell invasiveness, a wound healing assay and a Boyden chamber assay were done after FAKCD transfection. Results : We observed higher FAK expression in the chondrosarcoma cells than in chondrocytes. The level of attachment to type II collagen was significantly inhibited by blocking with the antibody of integrin- 1 and synthetic RGD peptides. Also, the adenovirus mediated transfection of FAK-CD resulted in the inhibition of the phosphorylation of FAK and significant inhibition of cell attachment in only JJ012, without changing FAK expression. Moreover, migration after transfection with FAK-CD was reduced by up to 79.9% for JJ012 and 75.5% for 105KC. Conclusion : Attachment of chondrosarcoma cells could be mediated through integrin- 1. We conclude that modified FAK expression contributes to the suppression of tumor cell attachment and invasion. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 552~558 | - |
dc.relation.isPartOf | Journal of the Korean Orthopaedic Association (대한정형외과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Focal adhesion kinase 발현 조절을 통한 연골육종 세포주의 침습성 조절 | - |
dc.title.alternative | The Control of Tumor Cell Invasiveness in Chondrosarcoma Cell Lines by Modifying Focal Adhesion Kinase Expression | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | 김윤희 | - |
dc.contributor.googleauthor | 박희붕 | - |
dc.contributor.googleauthor | 신규호 | - |
dc.contributor.googleauthor | 한수봉 | - |
dc.contributor.googleauthor | 이진우 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02086 | - |
dc.contributor.localId | A03230 | - |
dc.contributor.localId | A04293 | - |
dc.relation.journalcode | J01839 | - |
dc.subject.keyword | 연골육종 세포주, 세포 부착, 세포 침습, Focal adhesion kinase, Integrin- 1, FAK-CD | - |
dc.contributor.alternativeName | Shin, Kyoo Ho | - |
dc.contributor.alternativeName | Lee, Jin Woo | - |
dc.contributor.alternativeName | Hahn, Soo Bong | - |
dc.contributor.affiliatedAuthor | Shin, Kyoo Ho | - |
dc.contributor.affiliatedAuthor | Lee, Jin Woo | - |
dc.contributor.affiliatedAuthor | Hahn, Soo Bong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 37 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 552 | - |
dc.citation.endPage | 558 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Orthopaedic Association (대한정형외과학회지), Vol.37(4) : 552-558, 2002 | - |
dc.identifier.rimsid | 55564 | - |
dc.type.rims | ART | - |
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