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Cell specific Cre-mediated activation of the diphtheria toxin gene in pituitary tumor: Potential for cytotoxic gene therapy

DC FieldValueLanguage
dc.contributor.author이은직-
dc.date.accessioned2016-05-16T11:13:03Z-
dc.date.available2016-05-16T11:13:03Z-
dc.date.issued2002-
dc.identifier.issn1043-0342-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/144054-
dc.description.abstractDiphtheria toxin has been suggested for the treatment of malignant cancer. In this paper, we describe a strategy for targeting the expression of the diphtheria toxin gene to growth hormone (GH)-producing pituitary tumor cells using adenoviral vectors. We generated adenoviral vectors in which a stuffer DNA fragment, flanked by two loxP sequences, was placed between the GH or cytomegalovirus (CMV) promoters and the diphtheria toxin gene (GH-loxP-DT, and CMV-loxP-DT) or the beta-Gal gene (GH-loxP-Gal, and CMV-loxP-Gal). Co-infection of GH-loxP-DT with either CMV-Cre or GH-Cre induced cytotoxicity that was limited to GH4 cells. Little or no cytopathic effect was seen in GH4 cells infected with control viruses (CMV-loxP-Gal or GH-loxP-Gal with CMV-Cre or GH-Cre). To test the effectiveness of this strategy in vivo, GH4 cells were transplanted into nude mice. Intratumoral co-injection of adenoviruses carrying diphtheria toxin (GH-loxP-DT, and CMV-loxP-DT) and Cre recombinase (GH-Cre, and CMV-Cre) caused rapid regression of the transplanted GH4 tumors. These results indicate that Cre-mediated activation of a loxP-repressed form of the DT gene provides a useful strategy for targeted suicide gene therapy. This approach may be useful for GH-secreting adenomas and should be applicable to other neoplastic disorders-
dc.description.statementOfResponsibilityopen-
dc.format.extent533~542-
dc.relation.isPartOfHUMAN GENE THERAPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenoviridae-
dc.subject.MESHAnimals-
dc.subject.MESHCell Death/genetics-
dc.subject.MESHDiphtheria Toxin/genetics*-
dc.subject.MESHGene Expression Regulation, Neoplastic-
dc.subject.MESHGene Transfer Techniques-
dc.subject.MESHGenetic Therapy-
dc.subject.MESHGenetic Vectors-
dc.subject.MESHGrowth Hormone/biosynthesis-
dc.subject.MESHGrowth Hormone/genetics-
dc.subject.MESHIntegrases/genetics*-
dc.subject.MESHMice-
dc.subject.MESHMice, Nude-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHPituitary Neoplasms/genetics*-
dc.subject.MESHPituitary Neoplasms/metabolism-
dc.subject.MESHPituitary Neoplasms/pathology-
dc.subject.MESHPituitary Neoplasms/therapy-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHTumor Cells, Cultured-
dc.subject.MESHViral Proteins/genetics*-
dc.titleCell specific Cre-mediated activation of the diphtheria toxin gene in pituitary tumor: Potential for cytotoxic gene therapy-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorEun Jig Lee-
dc.contributor.googleauthorJ. Larry Jameson-
dc.identifier.doi10.1089/10430340252809829-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03050-
dc.relation.journalcodeJ01006-
dc.identifier.eissn1557-7422-
dc.identifier.pmid11874631-
dc.identifier.urlhttp://online.liebertpub.com/doi/abs/10.1089/10430340252809829-
dc.contributor.alternativeNameLee, Eun Jig-
dc.contributor.affiliatedAuthorLee, Eun Jig-
dc.rights.accessRightsnot free-
dc.citation.volume13-
dc.citation.number4-
dc.citation.startPage533-
dc.citation.endPage542-
dc.identifier.bibliographicCitationHUMAN GENE THERAPY, Vol.13(4) : 533-542, 2002-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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