Cited 41 times in
Cell specific Cre-mediated activation of the diphtheria toxin gene in pituitary tumor: Potential for cytotoxic gene therapy
DC Field | Value | Language |
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dc.contributor.author | 이은직 | - |
dc.date.accessioned | 2016-05-16T11:13:03Z | - |
dc.date.available | 2016-05-16T11:13:03Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1043-0342 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144054 | - |
dc.description.abstract | Diphtheria toxin has been suggested for the treatment of malignant cancer. In this paper, we describe a strategy for targeting the expression of the diphtheria toxin gene to growth hormone (GH)-producing pituitary tumor cells using adenoviral vectors. We generated adenoviral vectors in which a stuffer DNA fragment, flanked by two loxP sequences, was placed between the GH or cytomegalovirus (CMV) promoters and the diphtheria toxin gene (GH-loxP-DT, and CMV-loxP-DT) or the beta-Gal gene (GH-loxP-Gal, and CMV-loxP-Gal). Co-infection of GH-loxP-DT with either CMV-Cre or GH-Cre induced cytotoxicity that was limited to GH4 cells. Little or no cytopathic effect was seen in GH4 cells infected with control viruses (CMV-loxP-Gal or GH-loxP-Gal with CMV-Cre or GH-Cre). To test the effectiveness of this strategy in vivo, GH4 cells were transplanted into nude mice. Intratumoral co-injection of adenoviruses carrying diphtheria toxin (GH-loxP-DT, and CMV-loxP-DT) and Cre recombinase (GH-Cre, and CMV-Cre) caused rapid regression of the transplanted GH4 tumors. These results indicate that Cre-mediated activation of a loxP-repressed form of the DT gene provides a useful strategy for targeted suicide gene therapy. This approach may be useful for GH-secreting adenomas and should be applicable to other neoplastic disorders | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 533~542 | - |
dc.relation.isPartOf | HUMAN GENE THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Death/genetics | - |
dc.subject.MESH | Diphtheria Toxin/genetics* | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic | - |
dc.subject.MESH | Gene Transfer Techniques | - |
dc.subject.MESH | Genetic Therapy | - |
dc.subject.MESH | Genetic Vectors | - |
dc.subject.MESH | Growth Hormone/biosynthesis | - |
dc.subject.MESH | Growth Hormone/genetics | - |
dc.subject.MESH | Integrases/genetics* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Nude | - |
dc.subject.MESH | Mice, Transgenic | - |
dc.subject.MESH | Pituitary Neoplasms/genetics* | - |
dc.subject.MESH | Pituitary Neoplasms/metabolism | - |
dc.subject.MESH | Pituitary Neoplasms/pathology | - |
dc.subject.MESH | Pituitary Neoplasms/therapy | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.subject.MESH | Viral Proteins/genetics* | - |
dc.title | Cell specific Cre-mediated activation of the diphtheria toxin gene in pituitary tumor: Potential for cytotoxic gene therapy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Eun Jig Lee | - |
dc.contributor.googleauthor | J. Larry Jameson | - |
dc.identifier.doi | 10.1089/10430340252809829 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03050 | - |
dc.relation.journalcode | J01006 | - |
dc.identifier.eissn | 1557-7422 | - |
dc.identifier.pmid | 11874631 | - |
dc.identifier.url | http://online.liebertpub.com/doi/abs/10.1089/10430340252809829 | - |
dc.contributor.alternativeName | Lee, Eun Jig | - |
dc.contributor.affiliatedAuthor | Lee, Eun Jig | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 13 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 533 | - |
dc.citation.endPage | 542 | - |
dc.identifier.bibliographicCitation | HUMAN GENE THERAPY, Vol.13(4) : 533-542, 2002 | - |
dc.identifier.rimsid | 55551 | - |
dc.type.rims | ART | - |
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