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Purification and Chemical Modification of the Xylanase from Alkali-tolerant Bacillus sp. YA-14
DC Field | Value | Language |
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dc.contributor.author | 김동석 | - |
dc.date.accessioned | 2016-05-16T11:10:22Z | - |
dc.date.available | 2016-05-16T11:10:22Z | - |
dc.date.issued | 1994 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143952 | - |
dc.description.abstract | The xylanase from alkali-tolerant Bacillus sp. YA-14 was purified to homogeneity by CM-cellulose, Sephadex G-50, and hydroxyapatite column chromatographies. The molecular weight of the purified enzyme was estimated to be 20, 000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified enzyme slightly hydrolyzed carboxymethyl cellulose and Avicel, but did not hydrolyze soluble starch, dextran, pullulan, and ρ−nitrophenyl−β -D-xylopyranoside. The maximum degree of hydrolysis by enzyme for birchwood xylan and oat spelts xylan were 47 and 40%, respectively. The Michaelis constants for birchwood xylan and oat spelts xylan were calculated to be 3.03 mg/ml and 5.0 mg/ml, respectively. The activity of the xylanase was inhibited reversibly by HgCl 2 , and showed competitive inhibition by N-bromosuccinimide, which probably indicates the involvement of tryptophan residue in the active center of the enzyme. The Xylanase was identified to be xylose-producing endo-type xylanase and did not show the enzymatic activities which cleave the branch point of the xylan structure. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 41~48 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Purification and Chemical Modification of the Xylanase from Alkali-tolerant Bacillus sp. YA-14 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학) | - |
dc.contributor.googleauthor | Young Seo Park | - |
dc.contributor.googleauthor | Do Young Yum | - |
dc.contributor.googleauthor | Byoung Kwon Hahm | - |
dc.contributor.googleauthor | Dong Hoon Bai | - |
dc.contributor.googleauthor | Ju Hyun Yu | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00402 | - |
dc.relation.journalcode | J01594 | - |
dc.identifier.eissn | 1738-8872 | - |
dc.contributor.alternativeName | Kim, Dong Seok | - |
dc.contributor.affiliatedAuthor | Kim, Dong Seok | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 4 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 41 | - |
dc.citation.endPage | 48 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Vol.4(1) : 41-48, 1994 | - |
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