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Imipenem-EDTA disk method for differentiation of metallo-beta-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp.

Authors
 Dongeun Yong  ;  Kyungwon Lee  ;  Jong Hwa Yum  ;  Hee Bong Shin  ;  Gian Maria Rossolini  ;  Yunsop Chong 
Citation
 JOURNAL OF CLINICAL MICROBIOLOGY, Vol.40(10) : 3798-3801, 2002 
Journal Title
 JOURNAL OF CLINICAL MICROBIOLOGY 
ISSN
 0095-1137 
Issue Date
2002
MeSH
Acinetobacter/enzymology* ; Acinetobacter/isolation & purification ; Drug Resistance/physiology ; Edetic Acid/chemistry ; Humans ; Imipenem/metabolism* ; Pseudomonas/enzymology* ; Pseudomonas/isolation & purification ; Reagent Kits, Diagnostic ; Sensitivity and Specificity ; beta-Lactamases/metabolism*
Abstract
Rapid detection of metallo-β-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 μg of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and −20°C, respectively.
Files in This Item:
T200205875.pdf Download
DOI
10.1128/JCM.40.10.3798-3801.2002
Appears in Collections:
5. Research Institutes (연구소) > Research Institute of Bacterial Resistance (세균내성연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yum, Jong Hwa(염종화)
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143914
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