Cited 16 times in
Activation of extracellular signal regulated kinase 1/2 in human dermal microvascular endothelial cells stimulated by anti-endothelial cell antibodies in sera of patients with Behçet's disease
DC Field | Value | Language |
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dc.contributor.author | 방동식 | - |
dc.contributor.author | 이광훈 | - |
dc.date.accessioned | 2016-05-16T11:08:07Z | - |
dc.date.available | 2016-05-16T11:08:07Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0923-1811 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143865 | - |
dc.description.abstract | Anti-endothelial cell antibodies (AECA) have been detected in the sera of patients with Behçet's disease (BD). The isotype of AECA from BD is IgM recognizing 44 kDa antigen (IgM-AECA) of human dermal microvascular endothelial cells (HDMEC). After stimulation of HDMEC with AECA-positive sera from BD patients, the expression of intercellular cell adhesion molecule-1 (ICAM-1) on HDMEC increases significantly. Mitogen-activated protein kinase (MAPK) cascade is one of protein kinase families activated by a wide spectrum of extracellular stimuli. There are several subtypes, including extracellular signal regulated kinase (ERK)1/2, c-Jun NH2 terminal kinase (JNK), and p38 cascades, and they regulate various cellular processes such as cell growth, differentiation, and inflammation. We examined the involvement of MAPK as a signal transduction pathway in the IgM-AECA-induced ICAM-1 expression. We used enzyme-linked immunosorbent assay (ELISA) and fluorescence-activated cell sorting (FACS) for detecting the induction of ICAM-1 on HDMEC. We also examined the production of tumor necrosis factor α (TNFα) or interleukin-1α (IL-1α) by HDMEC after stimulation with IgM-AECA, and checked the involvement of MAPK by Western blot assay. IgM-AECA cocktail from 8 patients with BD induced expression of the ICAM-1 on HDMEC. Neither TNFα nor IL-1α was detected by ELISA, FACS or reverse transcriptase-polymerase chain reaction in activated HDMEC cultures. IgM-AECA cocktail activated ERK1/2 and showed peak activities at 5 min after the stimulation. Specific MAPK/ERK kinase inhibitor PD98059 inhibited IgM-AECA-induced ERK1/2 activities and ICAM-1 expression on HDMEC at a concentration of 60 μM. IgM-AECA can play a pathogenic role in induction of vasculitis and inflammatory lesions of BD by directly activating endothelial cells, not by production of TNFα or IL-1α from HDMEC. ERK1/2 are involved in expression of ICAM-1 on HDMEC stimulated with IgM-AECA. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF DERMATOLOGICAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Autoantibodies/pharmacology | - |
dc.subject.MESH | Behcet Syndrome/blood | - |
dc.subject.MESH | Behcet Syndrome/enzymology* | - |
dc.subject.MESH | Behcet Syndrome/genetics | - |
dc.subject.MESH | Endothelium, Vascular/enzymology* | - |
dc.subject.MESH | EnzymeActivation | - |
dc.subject.MESH | Gene Expression Regulation/immunology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intercellular Adhesion Molecule-1/genetics | - |
dc.subject.MESH | Interleukin-1/genetics | - |
dc.subject.MESH | Microcirculation/physiology | - |
dc.subject.MESH | Mitogen-Activated ProteinKinase1/metabolism* | - |
dc.subject.MESH | Mitogen-Activated ProteinKinase3 | - |
dc.subject.MESH | Mitogen-Activated ProteinKinases/metabolism* | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Reference Values | - |
dc.subject.MESH | Skin/blood supply* | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/genetics | - |
dc.title | Activation of extracellular signal regulated kinase 1/2 in human dermal microvascular endothelial cells stimulated by anti-endothelial cell antibodies in sera of patients with Behçet's disease | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학) | - |
dc.contributor.googleauthor | Kwang Hoon Lee | - |
dc.contributor.googleauthor | Heun Jung Cho | - |
dc.contributor.googleauthor | Hong Suk Kim | - |
dc.contributor.googleauthor | Won Jae Lee | - |
dc.contributor.googleauthor | Sungnack Lee | - |
dc.contributor.googleauthor | Dongsik Bang | - |
dc.identifier.doi | 10.1016/S0923-1811(02)00062-2 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01784 | - |
dc.contributor.localId | A02674 | - |
dc.relation.journalcode | J01370 | - |
dc.identifier.eissn | 1873-569X | - |
dc.identifier.pmid | 12354421 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0923181102000622 | - |
dc.subject.keyword | Antibodies | - |
dc.subject.keyword | Endothelial cells | - |
dc.subject.keyword | Behçet's disease | - |
dc.subject.keyword | ICAM-1 | - |
dc.subject.keyword | ERK 1/2 | - |
dc.contributor.alternativeName | Bang, Dong Sik | - |
dc.contributor.alternativeName | Lee, Kwang Hoon | - |
dc.contributor.affiliatedAuthor | Bang, Dong Sik | - |
dc.contributor.affiliatedAuthor | Lee, Kwang Hoon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 30 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 63 | - |
dc.citation.endPage | 72 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DERMATOLOGICAL SCIENCE, Vol.30(1) : 63-72, 2002 | - |
dc.identifier.rimsid | 37619 | - |
dc.type.rims | ART | - |
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