0 206

Cited 13 times in

Activation of extracellular signal regulated kinase 1/2 in human dermal microvascular endothelial cells stimulated by anti-endothelial cell antibodies in sera of patients with Behçet's disease

DC FieldValueLanguage
dc.contributor.author방동식-
dc.contributor.author이광훈-
dc.date.accessioned2016-05-16T11:08:07Z-
dc.date.available2016-05-16T11:08:07Z-
dc.date.issued2002-
dc.identifier.issn0923-1811-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/143865-
dc.description.abstractAnti-endothelial cell antibodies (AECA) have been detected in the sera of patients with Behçet's disease (BD). The isotype of AECA from BD is IgM recognizing 44 kDa antigen (IgM-AECA) of human dermal microvascular endothelial cells (HDMEC). After stimulation of HDMEC with AECA-positive sera from BD patients, the expression of intercellular cell adhesion molecule-1 (ICAM-1) on HDMEC increases significantly. Mitogen-activated protein kinase (MAPK) cascade is one of protein kinase families activated by a wide spectrum of extracellular stimuli. There are several subtypes, including extracellular signal regulated kinase (ERK)1/2, c-Jun NH2 terminal kinase (JNK), and p38 cascades, and they regulate various cellular processes such as cell growth, differentiation, and inflammation. We examined the involvement of MAPK as a signal transduction pathway in the IgM-AECA-induced ICAM-1 expression. We used enzyme-linked immunosorbent assay (ELISA) and fluorescence-activated cell sorting (FACS) for detecting the induction of ICAM-1 on HDMEC. We also examined the production of tumor necrosis factor α (TNFα) or interleukin-1α (IL-1α) by HDMEC after stimulation with IgM-AECA, and checked the involvement of MAPK by Western blot assay. IgM-AECA cocktail from 8 patients with BD induced expression of the ICAM-1 on HDMEC. Neither TNFα nor IL-1α was detected by ELISA, FACS or reverse transcriptase-polymerase chain reaction in activated HDMEC cultures. IgM-AECA cocktail activated ERK1/2 and showed peak activities at 5 min after the stimulation. Specific MAPK/ERK kinase inhibitor PD98059 inhibited IgM-AECA-induced ERK1/2 activities and ICAM-1 expression on HDMEC at a concentration of 60 μM. IgM-AECA can play a pathogenic role in induction of vasculitis and inflammatory lesions of BD by directly activating endothelial cells, not by production of TNFα or IL-1α from HDMEC. ERK1/2 are involved in expression of ICAM-1 on HDMEC stimulated with IgM-AECA.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF DERMATOLOGICAL SCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAutoantibodies/pharmacology-
dc.subject.MESHBehcet Syndrome/blood-
dc.subject.MESHBehcet Syndrome/enzymology*-
dc.subject.MESHBehcet Syndrome/genetics-
dc.subject.MESHEndothelium, Vascular/enzymology*-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHGene Expression Regulation/immunology-
dc.subject.MESHHumans-
dc.subject.MESHIntercellular Adhesion Molecule-1/genetics-
dc.subject.MESHInterleukin-1/genetics-
dc.subject.MESHMicrocirculation/physiology-
dc.subject.MESHMitogen-Activated Protein Kinase 1/metabolism*-
dc.subject.MESHMitogen-Activated Protein Kinase 3-
dc.subject.MESHMitogen-Activated Protein Kinases/metabolism*-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHReference Values-
dc.subject.MESHSkin/blood supply*-
dc.subject.MESHTumor Necrosis Factor-alpha/genetics-
dc.titleActivation of extracellular signal regulated kinase 1/2 in human dermal microvascular endothelial cells stimulated by anti-endothelial cell antibodies in sera of patients with Behçet's disease-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Dermatology (피부과학)-
dc.contributor.googleauthorKwang Hoon Lee-
dc.contributor.googleauthorHeun Jung Cho-
dc.contributor.googleauthorHong Suk Kim-
dc.contributor.googleauthorWon Jae Lee-
dc.contributor.googleauthorSungnack Lee-
dc.contributor.googleauthorDongsik Bang-
dc.identifier.doi10.1016/S0923-1811(02)00062-2-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01784-
dc.contributor.localIdA02674-
dc.relation.journalcodeJ01370-
dc.identifier.eissn1873-569X-
dc.identifier.pmid12354421-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0923181102000622-
dc.subject.keywordAntibodies-
dc.subject.keywordEndothelial cells-
dc.subject.keywordBehçet's disease-
dc.subject.keywordICAM-1-
dc.subject.keywordERK 1/2-
dc.contributor.alternativeNameBang, Dong Sik-
dc.contributor.alternativeNameLee, Kwang Hoon-
dc.contributor.affiliatedAuthorBang, Dong Sik-
dc.contributor.affiliatedAuthorLee, Kwang Hoon-
dc.rights.accessRightsnot free-
dc.citation.volume30-
dc.citation.number1-
dc.citation.startPage63-
dc.citation.endPage72-
dc.identifier.bibliographicCitationJOURNAL OF DERMATOLOGICAL SCIENCE, Vol.30(1) : 63-72, 2002-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.