Cited 4 times in
The Effect of α MSH Analogues on Rat Bones
DC Field | Value | Language |
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dc.contributor.author | 육종인 | - |
dc.contributor.author | 이유미 | - |
dc.date.accessioned | 2016-05-16T11:05:58Z | - |
dc.date.available | 2016-05-16T11:05:58Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143785 | - |
dc.description.abstract | Melanocortin is the downstream mediator of leptin signaling and absence of leptin signaling in ob/ob and db/db mice revealed the enhancement of bone formation through the central regulation. While α-melanocyte-stimulating hormone (α MSH) inhibits the secretion of interleukin-1 α and tumor necrosis factor-α from the inflammatory cells, α MSH can also enhance clonal expansion of pro B cells linked to stimulation of osteoclastogenesis. Therefore, we tested the effect of melanocortin on bones. α MSH analogues [6His] α MSH-ND and [6Asn] α MSH-ND were synthesized and the radio-ligand receptor binding- and cyclic AMP generating activity were analyzed in China Hamster Ovary cell line over-expressing melanocortin receptors. The EC50 of [6His] α MSH-ND measured from melanocortin-1, 3, 4 and 5 receptors were 0.008±0.0045, 1.523±0.707, 0.780±0.405, and 250.320±42.234 nM, respectively, and the EC50 of [6Asn] α MSH-ND were 16.8±6.94, 271.8±21.95, 8.0±1.21, and 1132.5±635.46 nM, respectively. Four weeks after the subcutaneous injection of the analogues, the body weights in the [6His] α MSH-ND and the [6Asn] α MSH-ND treated groups (346.0±20.63 g vs. 350.0±13.57 g) were lower than that of the vehicle treated group (375.8±17.31 g, p < 0.05). There was no difference in the total femoral BMD measured by dual x-ray absorptiometry among the three groups. Among the three groups, there were no differences in the total numbers of crystal violet positive- or alkaline phosphatase positive colonies, in the expression of Receptor Activator of Nuclear Factor Kappa-B ligand on the tibia and the total number of multinucleated osteoclast-like cells differentiated from primary cultured bone marrow cells. From the above results, no evidence of bone gain or loss was found after treatment of the α MSH analogues peripherally. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 500~510 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Body Weight/drug effects | - |
dc.subject.MESH | Bone and Bones/drug effects* | - |
dc.subject.MESH | CHO Cells | - |
dc.subject.MESH | Cricetinae | - |
dc.subject.MESH | Cyclic AMP/biosynthesis | - |
dc.subject.MESH | Eating/drug effects | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Osteoblasts/drug effects | - |
dc.subject.MESH | Osteoblasts/physiology | - |
dc.subject.MESH | Osteoclasts/drug effects | - |
dc.subject.MESH | Osteoclasts/physiology | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Receptors, Corticotropin/physiology | - |
dc.subject.MESH | Receptors, Melanocortin | - |
dc.subject.MESH | alpha-MSH/analogs & derivatives | - |
dc.subject.MESH | alpha-MSH/pharmacology* | - |
dc.title | The Effect of α MSH Analogues on Rat Bones | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Pathology (구강병리학) | - |
dc.contributor.googleauthor | Sung Kil Lim | - |
dc.contributor.googleauthor | Song Zhe Li | - |
dc.contributor.googleauthor | Yumie Rhee | - |
dc.contributor.googleauthor | Sang Su Chung | - |
dc.contributor.googleauthor | Yong Jun Jin | - |
dc.contributor.googleauthor | Jong In Yook | - |
dc.identifier.doi | 10.3349/ymj.2002.43.4.500 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02536 | - |
dc.contributor.localId | A03012 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 12205739 | - |
dc.subject.keyword | α MSH | - |
dc.subject.keyword | bone mineral density | - |
dc.subject.keyword | osteoclast | - |
dc.contributor.alternativeName | Yook, Jong In | - |
dc.contributor.alternativeName | Rhee, Yumie | - |
dc.contributor.affiliatedAuthor | Yook, Jong In | - |
dc.contributor.affiliatedAuthor | Rhee, Yumie | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 43 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 500 | - |
dc.citation.endPage | 510 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.43(4) : 500-510, 2002 | - |
dc.identifier.rimsid | 37548 | - |
dc.type.rims | ART | - |
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