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Gene expression pattern in Caco-2 cells following rotavirus infection

Authors
 Mariela A. Cuadras  ;  Dino A. Feigelstock  ;  Sungwhan An  ;  Harry B. Greenberg 
Citation
 JOURNAL OF VIROLOGY, Vol.76(9) : 4467-4482, 2002 
Journal Title
 JOURNAL OF VIROLOGY 
ISSN
 0022-538X 
Issue Date
2002
Abstract
Rotaviruses are recognized as the leading cause of severe dehydrating diarrhea in infants and young children worldwide. Preventive and therapeutic strategies are urgently needed to fight this pathogen. In tissue culture and in vivo, rotavirus induces structural and functional alterations in the host cell. In order to better understand the molecular mechanisms involved in the events after rotavirus infection, we identified host cellular genes whose mRNA levels changed after infection. For this analysis, we used microarrays containing more than 38,000 human cDNAs to study the transcriptional response of the human intestinal cell line Caco-2 to rotavirus infection. We found that 508 genes were differentially regulated >2-fold at 16 h after rotavirus infection, and only one gene was similarly regulated at 1 h postinfection. Of these transcriptional changes, 73% corresponded to the upregulation of genes, with the majority of them occurring late, at 12 or more hours postinfection. Some of the regulated genes were classified according to known biological function and included genes encoding integral membrane proteins, interferon-regulated genes, transcriptional and translational regulators, and calcium metabolism-related genes. A new picture of global transcriptional regulation in the infected cell is presented and families of genes which may be involved in viral pathogenesis are discussed.
Files in This Item:
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DOI
10.1128/JVI.76.9.4467-4482.2002
Appears in Collections:
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
Yonsei Authors
An, Sung Whan(안성환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143764
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