Effective isolation of magnesium lithospermate B and its inhibition of aldose reductase and fibronectin on mesangial cell line

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Authors: Mankil Jung ; Hyun Chul Lee ; Chul Woo Ahn ; Whuijung Park ; Sunhee Choi ; Hanjo Kim ; Donghak Cho ; Geun Taek Lee ; Hai Ri Li

MeSH: Aldehyde Reductase/antagonists & inhibitors* ; Aldehyde Reductase/metabolism ; Animals ; Cell Line ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/isolation & purification* ; Drugs, Chinese Herbal/pharmacology* ; Fibronectins/antagonists & inhibitors* ; Fibronectins/metabolism ; Glomerular Mesangium/cytology ; Glomerular Mesangium/drug effects* ; Glomerular Mesangium/enzymology* ; Mice ; Plant Extracts ; Plant Roots/chemistry ; Salvia miltiorrhiza

Keywords: magnesium lithospermate B ; aldose reductase ; fibronectin ; Salviae miltiorrhizae Radix ; antidiabetics

Abstract: We developed an effective isolation method of magnesium lithospermate B from Salviae miltiorrhizae Radix and found for the first time that magnesium lithospermate B shows strong in vitro inhibition (IC50=0.04 μM) of aldose reductase (AR), 2.5 times than that of clinically used epalrestat (IC50=0.1 μM) and accumulation of fibronectin dose dependantly.