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Der p 2 isoallergens have different allergenicity and quantification with 2-site ELISA using monoclonal antibodies is influenced by the isoallergens

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dc.description.abstractBackground Der p 2 isoallergens have been reported and the possibility of different allergenicity has also been suggested. In addition, the quantification with 2-site ELISA may be affected by the isoallergens. Objectives Two different recombinant Der p 2 (rDer p 2) isoallergens were compared in terms of human IgE responses and the reliability of quantification of them with two-site ELISA kits which use monoclonal antibodies (mAbs) as capture and detection of Der p 2. Methods Seven different Der p 2 cDNA from the cultured Dermatophagoides pteronyssinus (DP) were cloned and polymorphism in nine amino acid residues was found. Two different recombinant isoallergens (rDer p 2A and rDer p 2B) were expressed and compared to their human IgE immune responses by ELISA and the ELISA inhibition test with 23 sera of DP-allergic patients. The reliability of quantification of two different available 2-site ELISA kits, which used mAbs for capture and detection of Der p 2, was evaluated. Results The ELISA optical density of rDer p 2B-specific IgE (sIgE) was higher than that of rDer p 2A (P < 0.001). The ELISA inhibition curve of rDer p 2B sIgE in pool I sera (n = 5; high sIgE both to rDer p 2A and rDer p 2B) did not show any differences in the 50% inhibition concentration and maximum inhibitory percentage of rDer p 2A and rDer p 2B sIgE. However, with pool II sera (n = 5; markedly higher sIgE to rDer p 2B than rDer p 2A), the 50% inhibitory concentrations (10 µg/mL vs. 40 ng/mL) and maximum inhibitory percentage (61% vs. 99%) of rDer p 2B sIgE with the two recombinant isoallergens were quite different. rDer p 2B could be quantified with two different 2-site ELISA kits, but rDer p 2A was detected by only one kit. Conclusion We conclude that isoallergens of Der p 2 may have different IgE immune responses. Quantification of Der p 2 with 2-site ELISA kits that adopted mAbs, might be affected by the prevalent form of the isoallergens in reservoir dust.-
dc.relation.isPartOfClinical and Experimental Allergy-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleDer p 2 isoallergens have different allergenicity and quantification with 2-site ELISA using monoclonal antibodies is influenced by the isoallergens-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorJ.W. Park-
dc.contributor.googleauthorK.S. Kim-
dc.contributor.googleauthorH.S. Jin-
dc.contributor.googleauthorC.W. Kim-
dc.contributor.googleauthorD.B. Kang-
dc.contributor.googleauthorS. Y. Choi-
dc.contributor.googleauthorT. S. Yong-
dc.contributor.googleauthorS. H. Oh-
dc.contributor.googleauthorC.S. Hong-
dc.contributor.alternativeNamePark, Jung Won-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.alternativeNameHong, Chein Soo-
dc.contributor.affiliatedAuthorPark, Jung Won-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.contributor.affiliatedAuthorHong, Chein Soo-
dc.rights.accessRightsnot free-
dc.identifier.bibliographicCitationClinical and Experimental Allergy, Vol.32(7) : 1042-1047, 2002-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers


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