Cited 56 times in
Der p 2 isoallergens have different allergenicity and quantification with 2-site ELISA using monoclonal antibodies is influenced by the isoallergens
DC Field | Value | Language |
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dc.contributor.author | 박중원 | - |
dc.contributor.author | 용태순 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2016-05-16T10:57:30Z | - |
dc.date.available | 2016-05-16T10:57:30Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0954-7894 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143479 | - |
dc.description.abstract | Background Der p 2 isoallergens have been reported and the possibility of different allergenicity has also been suggested. In addition, the quantification with 2-site ELISA may be affected by the isoallergens. Objectives Two different recombinant Der p 2 (rDer p 2) isoallergens were compared in terms of human IgE responses and the reliability of quantification of them with two-site ELISA kits which use monoclonal antibodies (mAbs) as capture and detection of Der p 2. Methods Seven different Der p 2 cDNA from the cultured Dermatophagoides pteronyssinus (DP) were cloned and polymorphism in nine amino acid residues was found. Two different recombinant isoallergens (rDer p 2A and rDer p 2B) were expressed and compared to their human IgE immune responses by ELISA and the ELISA inhibition test with 23 sera of DP-allergic patients. The reliability of quantification of two different available 2-site ELISA kits, which used mAbs for capture and detection of Der p 2, was evaluated. Results The ELISA optical density of rDer p 2B-specific IgE (sIgE) was higher than that of rDer p 2A (P < 0.001). The ELISA inhibition curve of rDer p 2B sIgE in pool I sera (n = 5; high sIgE both to rDer p 2A and rDer p 2B) did not show any differences in the 50% inhibition concentration and maximum inhibitory percentage of rDer p 2A and rDer p 2B sIgE. However, with pool II sera (n = 5; markedly higher sIgE to rDer p 2B than rDer p 2A), the 50% inhibitory concentrations (10 µg/mL vs. 40 ng/mL) and maximum inhibitory percentage (61% vs. 99%) of rDer p 2B sIgE with the two recombinant isoallergens were quite different. rDer p 2B could be quantified with two different 2-site ELISA kits, but rDer p 2A was detected by only one kit. Conclusion We conclude that isoallergens of Der p 2 may have different IgE immune responses. Quantification of Der p 2 with 2-site ELISA kits that adopted mAbs, might be affected by the prevalent form of the isoallergens in reservoir dust. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1042~1047 | - |
dc.relation.isPartOf | CLINICAL AND EXPERIMENTAL ALLERGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Der p 2 isoallergens have different allergenicity and quantification with 2-site ELISA using monoclonal antibodies is influenced by the isoallergens | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | J.W. Park | - |
dc.contributor.googleauthor | K.S. Kim | - |
dc.contributor.googleauthor | H.S. Jin | - |
dc.contributor.googleauthor | C.W. Kim | - |
dc.contributor.googleauthor | D.B. Kang | - |
dc.contributor.googleauthor | S. Y. Choi | - |
dc.contributor.googleauthor | T. S. Yong | - |
dc.contributor.googleauthor | S. H. Oh | - |
dc.contributor.googleauthor | C.S. Hong | - |
dc.identifier.doi | 10.1046/j.1365-2222.2002.01421.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A02424 | - |
dc.contributor.localId | A04448-1 | - |
dc.relation.journalcode | J00548 | - |
dc.identifier.eissn | 1365-2222 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2222.2002.01421.x/abstract | - |
dc.subject.keyword | Dermatophagoides pteronyssinus | - |
dc.subject.keyword | Der p 2 | - |
dc.subject.keyword | isoallergen | - |
dc.subject.keyword | recombinant allergen | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 32 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1042 | - |
dc.citation.endPage | 1047 | - |
dc.identifier.bibliographicCitation | CLINICAL AND EXPERIMENTAL ALLERGY, Vol.32(7) : 1042-1047, 2002 | - |
dc.identifier.rimsid | 38190 | - |
dc.type.rims | ART | - |
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