164 235

Cited 16 times in

Gene cloning and biochemical analysis of thermostable chitosanase (TCH-2) from Bacillus coagulans CK108

DC FieldValueLanguage
dc.contributor.author윤호근-
dc.date.accessioned2016-05-16T10:55:10Z-
dc.date.available2016-05-16T10:55:10Z-
dc.date.issued2002-
dc.identifier.issn0916-8451-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/143400-
dc.description.abstractThe DNA sequence of the thermostable chitosanase TCH-2 gene from Bacillus coagulans CK108 showed a 843-bp open reading frame that encodes a protein of 280 amino acids with a signal peptide corresponding to 32 kDa in size. The deduced amino acid sequence of the chitosanase from Bacillus coagulans CK108 has 61.6%, 48.0%, and 12.6% identities to those from Bacillus ehemensis, Bacillus circulans, and Bacillus subtilis, respectively. C-Terminal homology analysis shows that the enzyme belongs to the Cluster I group. The size of the gene was similar to those from mesophiles of the Cluster I group with regard to higher preference for codons ending in G or C. The recombinant chitosanase was electrophoretically purified to homogeneity by only two steps with column chromatography. The half-life of the enzyme was 40 min at 90°C. The purified protein was also highly stable, retaining above 50% residual activities during treatment with denaturants such as urea (8 M) and guanidine•HCl (4 M) at 37°C for 30 min. The enzyme had a useful reactivity and a high specific activity for producing functional oligosaccharides as well, producing the tetramer as a major product.-
dc.description.statementOfResponsibilityopen-
dc.format.extent986~995-
dc.relation.isPartOfBIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleGene cloning and biochemical analysis of thermostable chitosanase (TCH-2) from Bacillus coagulans CK108-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorHo Geun Yoon, Kyung Han Lee, Hee Yun Kim, Hye Kyung Kim, Dong Hoon Shin, Bum Shik Hong , Hong Yon CHo-
dc.identifier.doi10.1271/bbb.66.986-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02625-
dc.relation.journalcodeJ00328-
dc.identifier.eissn1347-6947-
dc.subject.keywordhermostable chitosanase-
dc.subject.keywordGene cloning-
dc.subject.keywordNucleotide sequencing-
dc.subject.keywordBacillus coagulans CK108-
dc.subject.keywordChitosan oligosaccharide-
dc.contributor.alternativeNameYoon, Ho Geun-
dc.contributor.affiliatedAuthorYoon, Ho Geun-
dc.rights.accessRightsfree-
dc.citation.volume66-
dc.citation.number5-
dc.citation.startPage986-
dc.citation.endPage995-
dc.identifier.bibliographicCitationBIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Vol.66(5) : 986-995, 2002-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.