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Gene cloning and biochemical analysis of thermostable chitosanase (TCH-2) from Bacillus coagulans CK108
DC Field | Value | Language |
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dc.contributor.author | 윤호근 | - |
dc.date.accessioned | 2016-05-16T10:55:10Z | - |
dc.date.available | 2016-05-16T10:55:10Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143400 | - |
dc.description.abstract | The DNA sequence of the thermostable chitosanase TCH-2 gene from Bacillus coagulans CK108 showed a 843-bp open reading frame that encodes a protein of 280 amino acids with a signal peptide corresponding to 32 kDa in size. The deduced amino acid sequence of the chitosanase from Bacillus coagulans CK108 has 61.6%, 48.0%, and 12.6% identities to those from Bacillus ehemensis, Bacillus circulans, and Bacillus subtilis, respectively. C-Terminal homology analysis shows that the enzyme belongs to the Cluster I group. The size of the gene was similar to those from mesophiles of the Cluster I group with regard to higher preference for codons ending in G or C. The recombinant chitosanase was electrophoretically purified to homogeneity by only two steps with column chromatography. The half-life of the enzyme was 40 min at 90°C. The purified protein was also highly stable, retaining above 50% residual activities during treatment with denaturants such as urea (8 M) and guanidine•HCl (4 M) at 37°C for 30 min. The enzyme had a useful reactivity and a high specific activity for producing functional oligosaccharides as well, producing the tetramer as a major product. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 986~995 | - |
dc.relation.isPartOf | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Gene cloning and biochemical analysis of thermostable chitosanase (TCH-2) from Bacillus coagulans CK108 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Ho Geun Yoon, Kyung Han Lee, Hee Yun Kim, Hye Kyung Kim, Dong Hoon Shin, Bum Shik Hong , Hong Yon CHo | - |
dc.identifier.doi | 10.1271/bbb.66.986 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02625 | - |
dc.relation.journalcode | J00328 | - |
dc.identifier.eissn | 1347-6947 | - |
dc.subject.keyword | hermostable chitosanase | - |
dc.subject.keyword | Gene cloning | - |
dc.subject.keyword | Nucleotide sequencing | - |
dc.subject.keyword | Bacillus coagulans CK108 | - |
dc.subject.keyword | Chitosan oligosaccharide | - |
dc.contributor.alternativeName | Yoon, Ho Geun | - |
dc.contributor.affiliatedAuthor | Yoon, Ho Geun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 66 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 986 | - |
dc.citation.endPage | 995 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Vol.66(5) : 986-995, 2002 | - |
dc.identifier.rimsid | 53141 | - |
dc.type.rims | ART | - |
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