Cited 81 times in
Peroxisomal proliferator-activated receptor-gamma upregulates glucokinase gene expression in beta-cells
DC Field | Value | Language |
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dc.contributor.author | 김재우 | - |
dc.date.accessioned | 2016-05-16T10:52:42Z | - |
dc.date.available | 2016-05-16T10:52:42Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0012-1797 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143318 | - |
dc.description.abstract | Thiazolidinediones, synthetic ligands of peroxisomal proliferator-activated receptor-gamma (PPAR-gamma), improve peripheral insulin sensitivity and glucose-stimulated insulin secretion in pancreatic beta-cells. To explore the role of PPAR-gamma in glucose sensing of beta-cells, we have dissected the beta-cell-specific glucokinase (betaGK) promoter, which constitutes glucose-sensing apparatus in pancreatic beta-cells, and identified a peroxisomal proliferator response element (PPRE) in the promoter. The betaGK-PPRE is located in the region between +47 and +68 bp. PPAR-gamma/retinoid X receptor-alpha heterodimer binds to the element and activates the betaGK promoter. The betaGK promoter lacking or having mutations in PPRE cannot be activated by PPAR-gamma. PPAR-gamma activates the betaGK promoter in beta-cells as well as non-beta-cells. Furthermore, troglitazone increases endogenous GK expression and its enzyme activity in beta-cell lines. These results indicate that PPAR-gamma can regulate GK expression in beta-cells. Taking these results together with our previous work, we conclude that PPAR-gamma regulates gene expression of glucose-sensing apparatus and thereby improves glucose-sensing ability of beta-cells, contributing to the restoration of beta-cell function in type 2 diabetic subjects by troglitazone. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 676~685 | - |
dc.relation.isPartOf | DIABETES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Chromans/pharmacology | - |
dc.subject.MESH | DNA/metabolism | - |
dc.subject.MESH | Dimerization | - |
dc.subject.MESH | Enzyme Activation/drug effects | - |
dc.subject.MESH | GeneExpression/drug effects | - |
dc.subject.MESH | Glucokinase/genetics* | - |
dc.subject.MESH | Hypoglycemic Agents/pharmacology | - |
dc.subject.MESH | Islets of Langerhans/enzymology* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mutagenesis, Site-Directed | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Receptors, Cytoplasmic and Nuclear/metabolism | - |
dc.subject.MESH | Receptors, Cytoplasmic and Nuclear/physiology* | - |
dc.subject.MESH | Receptors, Retinoic Acid/metabolism | - |
dc.subject.MESH | Response Elements | - |
dc.subject.MESH | Retinoid X Receptors | - |
dc.subject.MESH | Thiazoles/pharmacology | - |
dc.subject.MESH | Thiazolidinediones* | - |
dc.subject.MESH | Transcription Factors/metabolism | - |
dc.subject.MESH | Transcription Factors/pharmacology | - |
dc.subject.MESH | Transcription Factors/physiology* | - |
dc.subject.MESH | Transcriptional Activation | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Troglitazone | - |
dc.title | Peroxisomal proliferator-activated receptor-gamma upregulates glucokinase gene expression in beta-cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Ha-il Kim | - |
dc.contributor.googleauthor | Ji-Young Cha | - |
dc.contributor.googleauthor | So-Youn Kim | - |
dc.contributor.googleauthor | Jae-woo Kim | - |
dc.contributor.googleauthor | Kyung Jin Roh | - |
dc.contributor.googleauthor | Je-Kyung Seong | - |
dc.contributor.googleauthor | Nam Taek Lee | - |
dc.contributor.googleauthor | Kang-Yell Choi | - |
dc.contributor.googleauthor | Kyung-Sup Kim | - |
dc.contributor.googleauthor | Yong-ho Ahn | - |
dc.identifier.doi | 10.2337/diabetes.51.3.676 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00865 | - |
dc.relation.journalcode | J00718 | - |
dc.identifier.eissn | 1939-327X | - |
dc.identifier.pmid | 11872666 | - |
dc.identifier.url | http://diabetes.diabetesjournals.org/content/51/3/676.long | - |
dc.contributor.alternativeName | Kim, Jae Woo | - |
dc.contributor.affiliatedAuthor | Kim, Jae Woo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 51 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 676 | - |
dc.citation.endPage | 685 | - |
dc.identifier.bibliographicCitation | DIABETES, Vol.51(3) : 676-685, 2002 | - |
dc.identifier.rimsid | 53064 | - |
dc.type.rims | ART | - |
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