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Proteome analysis of metabolically engineered Escherichia coli producing poly(3-hydroxybutyrate)

Authors
 Mee-Jung Han  ;  Sang Sun Yoon  ;  Sang Yup Lee 
Citation
 JOURNAL OF BACTERIOLOGY, Vol.183(1) : 301-308, 2001 
Journal Title
JOURNAL OF BACTERIOLOGY
ISSN
 0021-9193 
Issue Date
2001
MeSH
Bacterial Proteins/metabolism ; Electrophoresis, Gel, Two-Dimensional/methods ; Escherichia coli/genetics* ; Escherichia coli/growth & development ; Escherichia coli/metabolism* ; Genetic Engineering* ; Hydroxybutyrates/metabolism* ; Proteome*/metabolism* ; Recombination, Genetic ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Abstract
Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early stationary phase were analyzed at the protein level by two-dimensional gel electrophoresis. Out of 20 proteins showing altered expression levels with the accumulation of PHB, 13 proteins were identified with the aid of mass spectrometry. Three heat shock proteins, GroEL, GroES, and DnaK, were significantly up-regulated in PHB-accumulating cells. Proteins which play essential roles in protein biosynthesis were unfavorably influenced by the accumulation of PHB. Cellular demand for the large amount of acetyl coenzyme A and NADPH for the PHB biosynthesis resulted in the increased synthesis of two enzymes of the glycolytic pathway and one enzyme of the Entner-Doudoroff pathway. The expression of the yfiD gene encoding a 14.3-kDa protein, which is known to be produced at low pH, was greatly induced with the accumulation of PHB. Therefore, it could be concluded that the accumulation of PHB in E. coli acted as a stress on the cells, which reduced the cells' ability to synthesize proteins and induced the expression of various protective proteins.
Files in This Item:
T200103880.pdf Download
DOI
10.1128/JB.183.1.301-308.2001
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143205
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