Cited 95 times in
12-Lipoxygenase is increased in glucose-stimulated mesangial cells and in experimental diabetic nephropathy
DC Field | Value | Language |
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dc.contributor.author | 강신욱 | - |
dc.date.accessioned | 2016-02-19T11:21:30Z | - |
dc.date.available | 2016-02-19T11:21:30Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0085-2538 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142968 | - |
dc.description.abstract | BACKGROUND: Arachidonic acid-derived 12-lipoxygenase (12-LO) products have potent growth and chemotactic properties. The present studies examined whether 12-LO and fibronectin are induced in cultured rat mesangial cells (MCs) exposed to high glucose and whether they are expressed in experimental diabetic nephropathy. METHODS: To determine the effect of high glucose on MC 12-LO mRNA and protein expression, rat MCs were incubated with RPMI medium containing 100 (NG) or 450 mg/dL glucose (HG). For animal studies, rats were injected with diluent (control) or streptozotocin. The latter were left untreated (DM) or treated with insulin (DM + I). At sacrifice after four months, GAPDH, 12-LO, and fibronectin mRNA were measured by competitive reverse transcription-polymerase chain reaction (RT-PCR) in microdissected glomeruli (G). Renal sections were semiquantitatively scored (0 to 4+) for diabetic changes and for 12-LO and fibronectin by immunohistochemistry. RESULTS: 12-LO mRNA expression in MC exposed to HG (12.71 +/- 1.17 attm/microL) and DM G (1.78 +/- 0.65 x 10-3 attm/glomerulus) was significantly higher than those of MCs in NG media (6.71 +/- 0.78 attm/microL) and control G (0.34 +/- 0.12 x 10-3 attm/glomerulus, P < 0.005), respectively. Western blot revealed a 1.7- and a 2.8-fold increase in MC and G 12-LO protein expression, respectively (P < 0.05). The immunohistochemistry score for G 12-LO and diabetic nephropathy score was significantly greater in DM and DM + I than controls. MC and G GAPDH mRNA remained unchanged. CONCLUSIONS: In MCs exposed to HG and in diabetic rat glomeruli, increments in 12-LO mRNA and protein are associated with changes modeling diabetic nephropathy. These findings suggest a role for the 12-LO pathway in the pathogenesis of diabetic nephropathy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1354~1362 | - |
dc.relation.isPartOf | KIDNEY INTERNATIONAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 12-Lipoxygenase is increased in glucose-stimulated mesangial cells and in experimental diabetic nephropathy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Shin-Wook Kang | - |
dc.contributor.googleauthor | Sharon G Adler | - |
dc.contributor.googleauthor | Cynthia C Nast | - |
dc.contributor.googleauthor | Janine Lapage | - |
dc.contributor.googleauthor | Jia-Li Gu | - |
dc.contributor.googleauthor | Jerry L Nadler | - |
dc.contributor.googleauthor | Rama Natarajan | - |
dc.identifier.doi | 10.1046/j.1523-1755.2001.0590041354.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00053 | - |
dc.relation.journalcode | J01941 | - |
dc.identifier.eissn | 1523-1755 | - |
dc.subject.keyword | glomerular microdissection | - |
dc.subject.keyword | extracellular matrix | - |
dc.subject.keyword | renal glo-meruli | - |
dc.subject.keyword | cell hypertrophy | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.affiliatedAuthor | Kang, Shin Wook | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 59 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1354 | - |
dc.citation.endPage | 1362 | - |
dc.identifier.bibliographicCitation | KIDNEY INTERNATIONAL, Vol.59(4) : 1354-1362, 2001 | - |
dc.identifier.rimsid | 38667 | - |
dc.type.rims | ART | - |
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