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아데노 바이러스 Cytosine Deaminase/Thymidine Kinase 융합 유전자의 항 종양효과
DC Field | Value | Language |
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dc.contributor.author | 장진우 | - |
dc.date.accessioned | 2016-02-19T11:19:45Z | - |
dc.date.available | 2016-02-19T11:19:45Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1225-8245 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142903 | - |
dc.description.abstract | Objective:We investigated the feasibility of a double suicide gene/prodrug therapy, involving direct introduction of the herpes simplex virus Type 1 thymidine kinase(TK) gene and the Escherichia coli cytosine deaminase(CD) gene, via a recombinant adenoviral vector and ganciclovir(GCV) and/or 5-fluorocytosine(5-FC) treatment, in C6 glioma cells. Methods:Efficient gene transfer and transduction of C6 glioma cells via a recombinant adenovirus were evaluated by infecting cells with adenovirus bearing the β-galactosidase gene and then staining cells with 5-bromo-4-chloro-3-indolyl-13-D-galactoside. CD/TK expression in cells infected with adenovirus bearing the CD/TK gene(ad-CD/TK) was examined by immunoblotting analysis. For in vitro cytotoxicity experiments, the cells were infected with ad-CD/TK or ad-ΔE1(as a control). After addition of a variety of concentrations of GCV and 5-FU, either separately or in combination, cell viability was determined by staining the cells with crystal violet solution 6 days after infection. Result:C6 glioma cells were efficiently transduced with recombinant adenoviral vector at multiplicities of infection of 200 or more. In vitro cytotoxicity of GCV and/or 5-FC, either alone or in combination, was exclusively observed in the cells transduced with ad-CD/TK. Obvious cytotoxicity(>50% inhibition) was observed in the presence of 5-FC at concentrations greater than 30ug/ml or GCV at concentrations greater than 0.3ug/ml at a multiplicity of infection of 100. Additionally, cytotoxicity in the presence of both GCV and 5-FC was greater than that after sinlge-prodrug treatments, indicating additive effects of the prodrug treatments. Conclusion:The administration of a double-suicide gene/prodrug therapy might have great potential in the treatment of brain tumors. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | S13~S19 | - |
dc.relation.isPartOf | Journal of Korean Neurosurgical Society (대한신경외과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 아데노 바이러스 Cytosine Deaminase/Thymidine Kinase 융합 유전자의 항 종양효과 | - |
dc.title.alternative | Antitumor Effect of an Adenoviral Cytosine Deaminase/Thymidine Kinase Fusion Gene in C6 Glioma Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학) | - |
dc.contributor.googleauthor | 김영우 | - |
dc.contributor.googleauthor | 최재영 | - |
dc.contributor.googleauthor | 장진우 | - |
dc.contributor.googleauthor | 박용구 | - |
dc.contributor.googleauthor | 정상섭 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03484 | - |
dc.relation.journalcode | J01521 | - |
dc.subject.keyword | Adenovirus | - |
dc.subject.keyword | β-Galactosidase | - |
dc.subject.keyword | Glioma | - |
dc.subject.keyword | Suicide gene therapy | - |
dc.contributor.alternativeName | Chang, Jin Woo | - |
dc.contributor.affiliatedAuthor | Chang, Jin Woo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 30 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 13 | - |
dc.citation.endPage | 19 | - |
dc.identifier.bibliographicCitation | Journal of Korean Neurosurgical Society (대한신경외과학회지), Vol.30(12) : 13-19, 2001 | - |
dc.identifier.rimsid | 39562 | - |
dc.type.rims | ART | - |
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