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아데노 바이러스 Cytosine Deaminase/Thymidine Kinase 융합 유전자의 항 종양효과

DC FieldValueLanguage
dc.contributor.author장진우-
dc.date.accessioned2016-02-19T11:19:45Z-
dc.date.available2016-02-19T11:19:45Z-
dc.date.issued2001-
dc.identifier.issn1225-8245-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/142903-
dc.description.abstractObjective:We investigated the feasibility of a double suicide gene/prodrug therapy, involving direct introduction of the herpes simplex virus Type 1 thymidine kinase(TK) gene and the Escherichia coli cytosine deaminase(CD) gene, via a recombinant adenoviral vector and ganciclovir(GCV) and/or 5-fluorocytosine(5-FC) treatment, in C6 glioma cells. Methods:Efficient gene transfer and transduction of C6 glioma cells via a recombinant adenovirus were evaluated by infecting cells with adenovirus bearing the β-galactosidase gene and then staining cells with 5-bromo-4-chloro-3-indolyl-13-D-galactoside. CD/TK expression in cells infected with adenovirus bearing the CD/TK gene(ad-CD/TK) was examined by immunoblotting analysis. For in vitro cytotoxicity experiments, the cells were infected with ad-CD/TK or ad-ΔE1(as a control). After addition of a variety of concentrations of GCV and 5-FU, either separately or in combination, cell viability was determined by staining the cells with crystal violet solution 6 days after infection. Result:C6 glioma cells were efficiently transduced with recombinant adenoviral vector at multiplicities of infection of 200 or more. In vitro cytotoxicity of GCV and/or 5-FC, either alone or in combination, was exclusively observed in the cells transduced with ad-CD/TK. Obvious cytotoxicity(>50% inhibition) was observed in the presence of 5-FC at concentrations greater than 30ug/ml or GCV at concentrations greater than 0.3ug/ml at a multiplicity of infection of 100. Additionally, cytotoxicity in the presence of both GCV and 5-FC was greater than that after sinlge-prodrug treatments, indicating additive effects of the prodrug treatments. Conclusion:The administration of a double-suicide gene/prodrug therapy might have great potential in the treatment of brain tumors.-
dc.description.statementOfResponsibilityopen-
dc.format.extentS13~S19-
dc.relation.isPartOfJournal of Korean Neurosurgical Society (대한신경외과학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title아데노 바이러스 Cytosine Deaminase/Thymidine Kinase 융합 유전자의 항 종양효과-
dc.title.alternativeAntitumor Effect of an Adenoviral Cytosine Deaminase/Thymidine Kinase Fusion Gene in C6 Glioma Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Neurosurgery (신경외과학)-
dc.contributor.googleauthor김영우-
dc.contributor.googleauthor최재영-
dc.contributor.googleauthor장진우-
dc.contributor.googleauthor박용구-
dc.contributor.googleauthor정상섭-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03484-
dc.relation.journalcodeJ01521-
dc.subject.keywordAdenovirus-
dc.subject.keywordβ-Galactosidase-
dc.subject.keywordGlioma-
dc.subject.keywordSuicide gene therapy-
dc.contributor.alternativeNameChang, Jin Woo-
dc.contributor.affiliatedAuthorChang, Jin Woo-
dc.rights.accessRightsfree-
dc.citation.volume30-
dc.citation.number12-
dc.citation.startPage13-
dc.citation.endPage19-
dc.identifier.bibliographicCitationJournal of Korean Neurosurgical Society (대한신경외과학회지), Vol.30(12) : 13-19, 2001-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers

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