Cited 19 times in
Cloning of Human Acetyl-CoA Carboxylase β Promoter and Its Regulation by Muscle Regulatory Factors
DC Field | Value | Language |
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dc.contributor.author | 김경섭 | - |
dc.contributor.author | 안용호 | - |
dc.date.accessioned | 2016-02-19T11:11:49Z | - |
dc.date.available | 2016-02-19T11:11:49Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142612 | - |
dc.description.abstract | The 280-kDa beta-isoform of acetyl-CoA carboxylase (ACCbeta) is predominantly expressed in heart and skeletal muscle, whereas the 265-kDa alpha-isoform (ACCalpha) is the major ACC in lipogenic tissues. The ACCbeta promoter showed myoblast-specific promoter activity and was strongly induced by MyoD in NIH3T3 cells. Serial deletions of the promoter revealed that MyoD acts on the E-boxes located at positions -498 to -403 and on the proximal region including the 5'-untranslated region. Destruction of the E-boxes at positions -498 to -403 by site-directed mutagenesis resulted in a significant decrease of MyoD responsiveness. The "TGAAA" at -32 to -28 and the region around the transcription start site play important roles in basal transcription, probably as a TATA box and an Inr element, respectively. Mutations of another E-box at -14 to -9 and a "GCCTGTCA" sequence at +17 to +24 drastically decreased the MyoD responsiveness. The novel cis-element GCCTGTCA was preferentially bound by MyoD homodimer in EMSA and conferred MyoD responsiveness to a luciferase reporter, which was repressed by the overexpression of E12. This finding is unique since activation via E-boxes is mediated by heterodimers of MyoD and E-proteins. We screened a human skeletal muscle cDNA library to isolate clones expressing proteins that bind to the region around the GCCTGTCA (+8 to +27) sequence, and isolated Myf4 and Myf6 cDNAs. Electrophoretic mobility shift assay showed that recombinant Myf4 and Myf6 bind to this novel cis-element. Moreover, transient expression of Myf6 induced significant activation on the ACCbeta promoter or an artificial promoter harboring this novel cis-element. These findings suggest that muscle regulatory factors, such as MyoD, Myf4, and Myf6, contribute to the muscle-specific expression of ACCbeta via E-boxes and the novel cis-element GCCTGTCA. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2576~2585 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acetyl-CoA Carboxylase/genetics* | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Binding Sites | - |
dc.subject.MESH | Cloning, Molecular | - |
dc.subject.MESH | Gene Expression Regulation, Enzymologic | - |
dc.subject.MESH | Gene Library | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Muscle, Skeletal | - |
dc.subject.MESH | MyoD Protein/metabolism* | - |
dc.subject.MESH | Myogenic Regulatory Factors/metabolism* | - |
dc.subject.MESH | Promoter Regions, Genetic* | - |
dc.subject.MESH | Protein Binding | - |
dc.title | Cloning of Human Acetyl-CoA Carboxylase β Promoter and Its Regulation by Muscle Regulatory Factors | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Jae-Jung Lee | - |
dc.contributor.googleauthor | Young-Ah Moon | - |
dc.contributor.googleauthor | Joo-Hun Ha | - |
dc.contributor.googleauthor | Do-Jun Yoon | - |
dc.contributor.googleauthor | Yong-Ho Ahn | - |
dc.contributor.googleauthor | Kyung-Sup Kim | - |
dc.identifier.doi | 10.1074/jbc.M007002200 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00297 | - |
dc.contributor.localId | A02249 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 11076940 | - |
dc.contributor.alternativeName | Kim, Kyung Sup | - |
dc.contributor.alternativeName | Ahn, Yong Ho | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Sup | - |
dc.contributor.affiliatedAuthor | Ahn, Yong Ho | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 276 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 2576 | - |
dc.citation.endPage | 2585 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.276(4) : 2576-2585, 2001 | - |
dc.identifier.rimsid | 31136 | - |
dc.type.rims | ART | - |
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