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아데노바이러스를 이용한 IGF-1, TGF-β1 유전자 전달에 대한 인간 추간판 세포의 기질 생성 반응
DC Field | Value | Language |
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dc.contributor.author | 문성환 | - |
dc.date.accessioned | 2016-02-19T11:09:15Z | - |
dc.date.available | 2016-02-19T11:09:15Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1229-5701 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142517 | - |
dc.description.abstract | Background : Genetic modification of cells through gene transfer gains popularity as a sophisticated delivery system in the management of musculoskeletal diseas e. Anabolic growth factors like IGF-1 BMP-2 OP-1, and TGF-β1 were candidate for therapeutic purposus regenerating matrix of intervertebral disc. TGF-β1, OP-1 and BMP-2 share same pathway i.e. Smad while IGF-1 utilize P13K pathway. Accordingly it is logical to use two cytokine encoding genes which using different sign al transduction pathway to upregulate matrix synthesis. Purpose : To elucidate the anabolic effect of the combination gene transfer(IGF-1 and TGF-β1 encoding gene) to human disc cells, cultured in alginate beads. Materials and Methods : Lumbar and cervical intervertebral disc tissue was obtained from surgical disc procedure from fifteen patients. After isolation and culture of the cells. cultures were transduced with first. Adenovirus-TGF-β1 construct(Ad/TGF-β1) and Ad/IGF-1 respectively, second, with combination of two viral constructs(AD/TGF-β1+Ad/IGF-1). Cultures treated with saline and Ad/luciferase served as control. Then cultures were incorporated into alginate beads. Exogenous TGF-β1(2 ng/ml) and IGF-1(100 ng/ml) were administered also. Newly synthesized proteoglycan was assessed using S35 incorporation using chromatography on Sephadex G-25 in PD-10 column Results : In cultures transduced with single therapeutic gene construct. there w ere statistically significant 2.9 fold(Ad/TGF-β1) and 1.8 fold(Ad/IGF-1) increase in newly synthesized proteoglycan comparing control(p<0.05). In culture transduced with double combination of therapeutic gene construct, there were 3.9 fold(Ad/TGF-β 1+Ad/IGF-1) increase in newly synthesized proteoglycan comparing control(p<0.05). Culture treated with TGF-β 1(2ng/ml) showed 3.9 fold increase and IGF-1(100 ng/ml) 2.9 fold increase, TGF-β1+IGF-1 4.3 fold increase in proteoglycan synthesis compared to control. Conclusion : Combination gene therapy provided efficient mechanism of upregulating matrix regeneration of the human intervertebral disc cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 195~201 | - |
dc.relation.isPartOf | Journal of Korean Society of Spine Surgery (대한척추외과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 아데노바이러스를 이용한 IGF-1, TGF-β1 유전자 전달에 대한 인간 추간판 세포의 기질 생성 반응 | - |
dc.title.alternative | Responsiveness of Human Intervertebral Disc Cells in Matrix Synthesis To Adenovirus - Mediated Gene Transfer ( IGF - 1 , TGF - f31 Encoding Genes ) | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | 이환모 | - |
dc.contributor.googleauthor | 김학선 | - |
dc.contributor.googleauthor | 김남현 | - |
dc.contributor.googleauthor | 박문수 | - |
dc.contributor.googleauthor | 김향 | - |
dc.contributor.googleauthor | 문성환 | - |
dc.contributor.googleauthor | 강응식 | - |
dc.identifier.doi | 10.4184/jkss.2001.8.3.195 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01365 | - |
dc.relation.journalcode | J01550 | - |
dc.subject.keyword | Combination gene therapy | - |
dc.subject.keyword | Intervertebral disc | - |
dc.subject.keyword | Proteoglycan | - |
dc.subject.keyword | TGF-ß1 | - |
dc.subject.keyword | IGF-1 | - |
dc.contributor.alternativeName | Moon, Seong Hwan | - |
dc.contributor.affiliatedAuthor | Moon, Seong Hwan | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 8 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 195 | - |
dc.citation.endPage | 201 | - |
dc.identifier.bibliographicCitation | Journal of Korean Society of Spine Surgery (대한척추외과학회지), Vol.8(3) : 195-201, 2001 | - |
dc.identifier.rimsid | 31059 | - |
dc.type.rims | ART | - |
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