Cited 51 times in
Dominant Negative ER Induces Apoptosis in GH4 Pituitary Lactotrope Cells and Inhibits Tumor Growth in Nude Mice
DC Field | Value | Language |
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dc.contributor.author | 이은직 | - |
dc.date.accessioned | 2016-02-19T11:07:41Z | - |
dc.date.available | 2016-02-19T11:07:41Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0013-7227 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142458 | - |
dc.description.abstract | The ER plays an important role in the proliferation and differentiation of lactotrope tumor cells. GH4 cells were infected with adenoviral vectors (AdL540Q and Ad1–536) to investigate the ability of dominant negative ER mutants to affect the regulation of gene expression and cell growth by endogenous ER. The dominant negative mutants suppressed estradiol stimulation of an estrogen-responsive reporter gene and the PRL promoter in these cells. AdL540Q or Ad1–536 infection also inhibited GH4 cell growth and induced apoptosis, increasing the expression of the proapoptotic Bax protein and decreasing the expression of antiapoptotic Bcl-2. AdwtER-infected cells also showed decreased Bcl-2 protein. E2-induced activation of p38 MAPK, an enzyme that may participate in apoptosis, was observed in cells infected with AdwtER, AdL540Q, and Ad1–536. Consistent with the apoptotic effects in vitro, infection of GH4 cells with AdL540Q or Ad1–536 inhibited the ability of the cells to form tumors in nude mice. These results indicate that dominant negative ER mutants induce apoptosis of GH4 cells and suppress tumor formation and development. The delivery of dominant negative ERs by adenoviral vectors may provide an alternative modality for the targeted therapy of pituitary lactotrope adenomas and other estrogen-responsive tumors. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 3756~3763 | - |
dc.relation.isPartOf | ENDOCRINOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Dominant Negative ER Induces Apoptosis in GH4 Pituitary Lactotrope Cells and Inhibits Tumor Growth in Nude Mice | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Eun Jig Lee | - |
dc.contributor.googleauthor | W. Rachel Duan | - |
dc.contributor.googleauthor | Monika Jakacka | - |
dc.contributor.googleauthor | Barry D. Gehm | - |
dc.contributor.googleauthor | J. Larry Jameson | - |
dc.identifier.doi | 10.1210/endo.142.9.8372 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03050 | - |
dc.relation.journalcode | J00772 | - |
dc.identifier.eissn | 1945-7170 | - |
dc.identifier.url | http://press.endocrine.org/doi/abs/10.1210/endo.142.9.8372 | - |
dc.contributor.alternativeName | Lee, Eun Jig | - |
dc.contributor.affiliatedAuthor | Lee, Eun Jig | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 142 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 3756 | - |
dc.citation.endPage | 3763 | - |
dc.identifier.bibliographicCitation | ENDOCRINOLOGY, Vol.142(9) : 3756-3763, 2001 | - |
dc.identifier.rimsid | 31016 | - |
dc.type.rims | ART | - |
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