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Bcl-2 blocks cisplatin-induced apoptosis by suppression of ERK-mediated p53 accumulation in B104 cells

DC FieldValueLanguage
dc.contributor.author안용호-
dc.contributor.author이병인-
dc.date.accessioned2016-02-19T11:00:28Z-
dc.date.available2016-02-19T11:00:28Z-
dc.date.issued2001-
dc.identifier.issn0169-328X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/142185-
dc.description.abstractBcl-2 has been reported to inhibit neurotoxicity induced by cisplatin. However, neither the mechanism of cisplatin-induced neurotoxicity nor the mechanism by which Bcl-2 confers neuroprotection is clear. In this study, the signaling pathways involved in cisplatin-induced neurotoxicity were examined using a rat neuroblastoma cell line, B104. Treatment of B104 cells with cisplatin induced apoptosis, accompanying the accumulation of p53 and Bax protein. Interestingly, extracellular signal-regulated kinase 1/2 (ERK1/2) activities of MAP kinases were markedly enhanced prior to cisplatin-induced accumulation of p53 and Bax. Inhibition of ERK1/2 activities using PD98059, a selective MEK inhibitor, blocked the apoptotic cell death preventing cisplatin-induced accumulation of p53 and Bax. These results suggest that ERK mediates cisplatin-induced p53 activation to trigger apoptosis in B104 cells. Overexpression of Bcl-2 in B104 cells resulted in the complete resistance to cisplatin-induced apoptosis blocking ERK activation and the subsequent signaling pathway of p53. Our study clearly demonstrates that the action site of Bcl-2 localizes upstream of ERK in cisplatin-induced apoptotic signaling pathway.-
dc.description.statementOfResponsibilityopen-
dc.format.extent18~26-
dc.relation.isPartOfMOLECULAR BRAIN RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntineoplastic Agents/pharmacology-
dc.subject.MESHApoptosis/drug effects-
dc.subject.MESHApoptosis/physiology*-
dc.subject.MESHCisplatin/pharmacology-
dc.subject.MESHGene Expression/physiology-
dc.subject.MESHMitogen-Activated Protein Kinases/metabolism*-
dc.subject.MESHNeuroblastoma*-
dc.subject.MESHProto-Oncogene Proteins/genetics-
dc.subject.MESHProto-Oncogene Proteins c-bcl-2/genetics*-
dc.subject.MESHRats-
dc.subject.MESHTumor Cells, Cultured-
dc.subject.MESHTumor Suppressor Protein p53/metabolism*-
dc.subject.MESHbcl-2-Associated X Protein-
dc.titleBcl-2 blocks cisplatin-induced apoptosis by suppression of ERK-mediated p53 accumulation in B104 cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorSun Ah Park-
dc.contributor.googleauthorHyun Jin Park-
dc.contributor.googleauthorByung In Lee-
dc.contributor.googleauthorYong Ho Ahn-
dc.contributor.googleauthorSeung U Kim-
dc.contributor.googleauthorKyeong Sook Choi-
dc.identifier.doi10.1016/S0169-328X(01)00176-0-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02249-
dc.contributor.localIdA02797-
dc.relation.journalcodeJ02252-
dc.identifier.pmid11532334-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0169328X01001760?np=y-
dc.subject.keywordApoptosis-
dc.subject.keywordBcl-2-
dc.subject.keywordCisplatin-
dc.subject.keywordExtracellular signal-regulated kinase-
dc.subject.keywordp53-
dc.subject.keywordB104 cell-
dc.contributor.alternativeNameAhn, Yong Ho-
dc.contributor.alternativeNameLee, Byung In-
dc.contributor.affiliatedAuthorAhn, Yong Ho-
dc.contributor.affiliatedAuthorLee, Byung In-
dc.rights.accessRightsnot free-
dc.citation.volume93-
dc.citation.number1-
dc.citation.startPage18-
dc.citation.endPage26-
dc.identifier.bibliographicCitationMOLECULAR BRAIN RESEARCH , Vol.93(1) : 18-26, 2001-
dc.identifier.rimsid31667-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers

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