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Confocal laser scanning microscopy를 이용한 표피내 칼슘기울기 관찰

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dc.contributor.author이승헌-
dc.date.accessioned2016-02-19T10:58:12Z-
dc.date.available2016-02-19T10:58:12Z-
dc.date.issued2001-
dc.identifier.issn0494-4739-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/142103-
dc.description.abstractBackground: Ion capture cytochemistry(the potassium oxalate pyroantimonate method), semiquantitatively, and proton probe X-ray microanalysis, quantitatively, have been applied to investigate the epidermal calcium distribution. Objective:In this study, we examined the epidermal calcium distribution with confocal laser scanning microscopy(CLSM) in an attempt to evaluate the possibility of another method in epidermal calcium study. Methods:The change of epidermal calcium distribution after barrier perturbation with tape stripping was investigated with CLSM and was compared to the results of ion capture cytochemistry. Results:The calcium distribution pattern in normal murine epidermis demonstrated by CLSM show a normal calcium gradient, from a low level of calcium ions in the basal and spinous layer, followed by a progressive increase with a level of calcium ions reaching its maximal density within the outer stratum granulosum. Disruption of the epidermal barrier with tape stripping induced an immediate loss of the calcium gradient and the calcium gradient after 36h was almost normalized, in parallel with the recovery of barrier function. Conclusion:These results show that calcium gradient in murine epidermis after tape-stripping is restored by 36h and CLSM study can be used as a new method in epidermal calcium study.-
dc.description.statementOfResponsibilityopen-
dc.format.extent22~27-
dc.relation.isPartOfKorean Journal of Dermatology (대한피부과학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleConfocal laser scanning microscopy를 이용한 표피내 칼슘기울기 관찰-
dc.title.alternativeThe Change of Epidermal Calcium Gradient : Confocal Laser Scanning Microscopic Approach-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Dermatology (피부과학)-
dc.contributor.googleauthor황상민-
dc.contributor.googleauthor안형진-
dc.contributor.googleauthor안성구-
dc.contributor.googleauthor이승헌-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02931-
dc.relation.journalcodeJ02000-
dc.subject.keywordcalcium gradient-
dc.subject.keywordconfocal laser scanning microsocopy-
dc.subject.keywordpermeability barrier-
dc.contributor.alternativeNameLee, Seung Hun-
dc.contributor.affiliatedAuthorLee, Seung Hun-
dc.rights.accessRightsfree-
dc.citation.volume39-
dc.citation.number1-
dc.citation.startPage22-
dc.citation.endPage27-
dc.identifier.bibliographicCitationKorean Journal of Dermatology (대한피부과학회지), Vol.39(1) : 22-27, 2001-
dc.identifier.rimsid31604-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers

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