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Thermostable Chitosanase from Bacillus sp. Strain CK4: Its Purification, Characterization, and Reaction Patterns

DC FieldValueLanguage
dc.contributor.author윤호근-
dc.date.accessioned2016-02-19T10:54:18Z-
dc.date.available2016-02-19T10:54:18Z-
dc.date.issued2001-
dc.identifier.issn0916-8451-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/141960-
dc.description.abstractA thermostable chitosanase, purified 156-fold to homogeneity in an overall yield of 12.4%, has a molecular weight of about 29,000±2,000, and is composed of monomer. The enzyme degraded soluble chitosan, colloidal chitosan, and glycol chitosan, but did not degrade chitin or other β-linked polymers. The enzyme activity was increased about 2.5-fold by the addition of 10 mM Co2+ and 1.4-fold by Mn2+. However, Cu2+ ion strongly inhibited the enzyme. Optimum temperature and pH were 60°C and 6.5, respectively. The enzyme was stable after heat treatment at 80°C for 30 min or 70°C for 60 min and fairly stable in protein denaturants as well. Chitosan was hydrolyzed to (GlcN)4 as a major product, by incubation with the purified enzyme. The effects of ammonium sulfate and organic solvents on the action pattern of the thermostable chitosanase were investigated. The amounts of (GlcN)3-(GlcN)6 were increased about 30% (w/w) in DAC 99 soluble chitosan containing 10% ammonium sulfate, and (GlcN)1 was not produced. The monophasic reaction system consisted of DAC 72 soluble chitosan in 10% EtOH also showed no formation of (GlcN)1, however, the yield of (GlcN)3 ∼ (GlcN)6 was lower than DAC 99 soluble chitosan-10% ammonium sulfate. The optimal concentration of ammonium sulfate to be added was 20%. At this concentration, the amount of hexamer was increased by over 12% compared to the water-salt free system.-
dc.description.statementOfResponsibilityopen-
dc.format.extent802~809-
dc.relation.isPartOfBIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmmonium Sulfate/chemistry-
dc.subject.MESHBacillus/enzymology*-
dc.subject.MESHChromatography, Gel-
dc.subject.MESHChromatography, Ion Exchange-
dc.subject.MESHChromatography, Thin Layer-
dc.subject.MESHGlycoside Hydrolases/chemistry*-
dc.subject.MESHHydrogen-Ion Concentration-
dc.subject.MESHIsoelectric Focusing-
dc.subject.MESHMetals/chemistry-
dc.subject.MESHMolecular Weight-
dc.subject.MESHOligosaccharides/chemistry-
dc.subject.MESHSubstrate Specificity-
dc.subject.MESHTemperature-
dc.titleThermostable Chitosanase from Bacillus sp. Strain CK4: Its Purification, Characterization, and Reaction Patterns-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorHo-Geun YOON-
dc.contributor.googleauthorHee-Yun KIM-
dc.contributor.googleauthorHye-Kyung KIM-
dc.contributor.googleauthorBum-Shik HONG-
dc.contributor.googleauthorDong-Hoon SHIN-
dc.contributor.googleauthorHong-Yon CHO-
dc.identifier.doi10.1271/bbb.65.802-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02625-
dc.relation.journalcodeJ00328-
dc.identifier.eissn1347-6947-
dc.identifier.pmid11388456-
dc.subject.keywordThermostable chitosanase-
dc.subject.keywordBacillus sp.strain CK4-
dc.subject.keywordChitosan oligosaccharide-
dc.contributor.alternativeNameYoon, Ho Geun-
dc.contributor.affiliatedAuthorYoon, Ho Geun-
dc.rights.accessRightsfree-
dc.citation.volume65-
dc.citation.number4-
dc.citation.startPage802-
dc.citation.endPage809-
dc.identifier.bibliographicCitationBIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Vol.65(4) : 802-809, 2001-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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