Cited 13 times in
Thermostable Chitosanase from Bacillus sp. Strain CK4: Its Purification, Characterization, and Reaction Patterns
DC Field | Value | Language |
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dc.contributor.author | 윤호근 | - |
dc.date.accessioned | 2016-02-19T10:54:18Z | - |
dc.date.available | 2016-02-19T10:54:18Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141960 | - |
dc.description.abstract | A thermostable chitosanase, purified 156-fold to homogeneity in an overall yield of 12.4%, has a molecular weight of about 29,000±2,000, and is composed of monomer. The enzyme degraded soluble chitosan, colloidal chitosan, and glycol chitosan, but did not degrade chitin or other β-linked polymers. The enzyme activity was increased about 2.5-fold by the addition of 10 mM Co2+ and 1.4-fold by Mn2+. However, Cu2+ ion strongly inhibited the enzyme. Optimum temperature and pH were 60°C and 6.5, respectively. The enzyme was stable after heat treatment at 80°C for 30 min or 70°C for 60 min and fairly stable in protein denaturants as well. Chitosan was hydrolyzed to (GlcN)4 as a major product, by incubation with the purified enzyme. The effects of ammonium sulfate and organic solvents on the action pattern of the thermostable chitosanase were investigated. The amounts of (GlcN)3-(GlcN)6 were increased about 30% (w/w) in DAC 99 soluble chitosan containing 10% ammonium sulfate, and (GlcN)1 was not produced. The monophasic reaction system consisted of DAC 72 soluble chitosan in 10% EtOH also showed no formation of (GlcN)1, however, the yield of (GlcN)3 ∼ (GlcN)6 was lower than DAC 99 soluble chitosan-10% ammonium sulfate. The optimal concentration of ammonium sulfate to be added was 20%. At this concentration, the amount of hexamer was increased by over 12% compared to the water-salt free system. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 802~809 | - |
dc.relation.isPartOf | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Ammonium Sulfate/chemistry | - |
dc.subject.MESH | Bacillus/enzymology* | - |
dc.subject.MESH | Chromatography, Gel | - |
dc.subject.MESH | Chromatography, Ion Exchange | - |
dc.subject.MESH | Chromatography, Thin Layer | - |
dc.subject.MESH | Glycoside Hydrolases/chemistry* | - |
dc.subject.MESH | Hydrogen-Ion Concentration | - |
dc.subject.MESH | Isoelectric Focusing | - |
dc.subject.MESH | Metals/chemistry | - |
dc.subject.MESH | Molecular Weight | - |
dc.subject.MESH | Oligosaccharides/chemistry | - |
dc.subject.MESH | Substrate Specificity | - |
dc.subject.MESH | Temperature | - |
dc.title | Thermostable Chitosanase from Bacillus sp. Strain CK4: Its Purification, Characterization, and Reaction Patterns | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Ho-Geun YOON | - |
dc.contributor.googleauthor | Hee-Yun KIM | - |
dc.contributor.googleauthor | Hye-Kyung KIM | - |
dc.contributor.googleauthor | Bum-Shik HONG | - |
dc.contributor.googleauthor | Dong-Hoon SHIN | - |
dc.contributor.googleauthor | Hong-Yon CHO | - |
dc.identifier.doi | 10.1271/bbb.65.802 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02625 | - |
dc.relation.journalcode | J00328 | - |
dc.identifier.eissn | 1347-6947 | - |
dc.identifier.pmid | 11388456 | - |
dc.subject.keyword | Thermostable chitosanase | - |
dc.subject.keyword | Bacillus sp.strain CK4 | - |
dc.subject.keyword | Chitosan oligosaccharide | - |
dc.contributor.alternativeName | Yoon, Ho Geun | - |
dc.contributor.affiliatedAuthor | Yoon, Ho Geun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 65 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 802 | - |
dc.citation.endPage | 809 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Vol.65(4) : 802-809, 2001 | - |
dc.identifier.rimsid | 35882 | - |
dc.type.rims | ART | - |
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