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The effects of arginase inhibitor on lung oxidative stress and inflammation caused by pneumoperitoneum in rats

DC FieldValueLanguage
dc.contributor.author나성원-
dc.contributor.author오영준-
dc.contributor.author조진선-
dc.date.accessioned2016-02-04T11:50:58Z-
dc.date.available2016-02-04T11:50:58Z-
dc.date.issued2015-
dc.identifier.issn1471-2253-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/141339-
dc.description.abstractBACKGROUND: Pneumoperitoneum-induced oxidative stress and organ injury are known to be associated with nitric oxide (NO) inactivation. Because arginase competes with NO synthase (NOS) for a common substrate, L-arginine, arginase inhibition may increase NO bioavailability. Therefore, we evaluated the ability of the arginase inhibitor, 2 (S)-amino-6-boronohexanoic acid (ABH), to attenuate pneumoperitoneum-induced decrease of NO bioavailability and lung injury. METHODS: Thirty rats were randomly divided into the following groups: 1) the PP-ABH group received a subcutaneous injection of ABH (5 mg/kg) 1 h before induction of pneumoperitoneum (insufflation to intraperitoneal pressure of 15 mmHg for 60 min); 2) the PP group received saline by subcutaneous injection 1 h before induction of pneumoperitoneum; and 3) the control group received saline by subcutaneous injection before a sham procedure with no gas insufflation. After desufflation, blood was collected to determine levels of plasma nitrite, NOS, inflammatory cytokines, and malondialdehyde, a marker of oxidative stress. Lung tissue was obtained for histological evaluation. RESULTS: We found that plasma nitrite levels were lower in the PP group and higher in the PP-ABH group, compared with controls (P <0.01 and P <0.05, respectively). In the PP group, endothelial NOS activity was decreased and inducible NOS activity was increased compared with the PP-ABH and control groups. Malondialdehyde levels increased 3-fold in the PP group and 2-fold in the PP-ABH group compared with controls. Tumor necrosis factor-α, interleukin-6, and interleukin-1ß levels were elevated in the PP group compared to the control group, but the increase in cytokine production was attenuated or blocked in the PP-ABH group. Lung injury scores were 4.8-fold higher in the PP group and 2-fold higher in the PP-ABH group compared with controls (P <0.001 and P <0.01, respectively). DISCUSSION: Pneumoperitoneum decreases NO bioavailability and increases the inflammation cytokines, resulting in organ injuries. Inhibition of arginase activity could maintain NO bioavailability by attenuating pneumoperitoneum-induced changes in NOS activity. In addition, arginase inhibition attenuated the oxidative stress and inflammation and decreased the severity of lung injury caused by pneumoperitoneum. CONCLUSIONS: By increasing NO bioavailability and suppressing oxidative stress and inflammation, pretreatment with an arginase inhibitor may protect against lung injury caused by pneumoperitoneum.-
dc.description.statementOfResponsibilityopen-
dc.format.extent129-
dc.relation.isPartOfBMC Anesthesiology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleThe effects of arginase inhibitor on lung oxidative stress and inflammation caused by pneumoperitoneum in rats-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Anesthesiology (마취통증의학)-
dc.contributor.googleauthorJin Sun Cho-
dc.contributor.googleauthorYoung Jun Oh-
dc.contributor.googleauthorOk Soo Kim-
dc.contributor.googleauthorSungwon Na-
dc.identifier.doi10.1186/s12871-015-0112-y-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01232-
dc.contributor.localIdA02389-
dc.contributor.localIdA03914-
dc.relation.journalcodeJ00349-
dc.contributor.alternativeNameNa, Sung Won-
dc.contributor.alternativeNameOh, Young Jun-
dc.contributor.alternativeNameCho, Jin Sun-
dc.contributor.affiliatedAuthorNa, Sung Won-
dc.contributor.affiliatedAuthorOh, Young Jun-
dc.contributor.affiliatedAuthorCho, Jin Sun-
dc.rights.accessRightsfree-
dc.citation.volume15-
dc.citation.startPage129-
dc.citation.endPage129-
dc.identifier.bibliographicCitationBMC Anesthesiology, Vol.15 : 129-129, 2015-
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실)

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