Cited 9 times in
The effects of arginase inhibitor on lung oxidative stress and inflammation caused by pneumoperitoneum in rats
DC Field | Value | Language |
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dc.contributor.author | 나성원 | - |
dc.contributor.author | 오영준 | - |
dc.contributor.author | 조진선 | - |
dc.date.accessioned | 2016-02-04T11:50:58Z | - |
dc.date.available | 2016-02-04T11:50:58Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141339 | - |
dc.description.abstract | BACKGROUND: Pneumoperitoneum-induced oxidative stress and organ injury are known to be associated with nitric oxide (NO) inactivation. Because arginase competes with NO synthase (NOS) for a common substrate, L-arginine, arginase inhibition may increase NO bioavailability. Therefore, we evaluated the ability of the arginase inhibitor, 2 (S)-amino-6-boronohexanoic acid (ABH), to attenuate pneumoperitoneum-induced decrease of NO bioavailability and lung injury. METHODS: Thirty rats were randomly divided into the following groups: 1) the PP-ABH group received a subcutaneous injection of ABH (5 mg/kg) 1 h before induction of pneumoperitoneum (insufflation to intraperitoneal pressure of 15 mmHg for 60 min); 2) the PP group received saline by subcutaneous injection 1 h before induction of pneumoperitoneum; and 3) the control group received saline by subcutaneous injection before a sham procedure with no gas insufflation. After desufflation, blood was collected to determine levels of plasma nitrite, NOS, inflammatory cytokines, and malondialdehyde, a marker of oxidative stress. Lung tissue was obtained for histological evaluation. RESULTS: We found that plasma nitrite levels were lower in the PP group and higher in the PP-ABH group, compared with controls (P <0.01 and P <0.05, respectively). In the PP group, endothelial NOS activity was decreased and inducible NOS activity was increased compared with the PP-ABH and control groups. Malondialdehyde levels increased 3-fold in the PP group and 2-fold in the PP-ABH group compared with controls. Tumor necrosis factor-α, interleukin-6, and interleukin-1ß levels were elevated in the PP group compared to the control group, but the increase in cytokine production was attenuated or blocked in the PP-ABH group. Lung injury scores were 4.8-fold higher in the PP group and 2-fold higher in the PP-ABH group compared with controls (P <0.001 and P <0.01, respectively). DISCUSSION: Pneumoperitoneum decreases NO bioavailability and increases the inflammation cytokines, resulting in organ injuries. Inhibition of arginase activity could maintain NO bioavailability by attenuating pneumoperitoneum-induced changes in NOS activity. In addition, arginase inhibition attenuated the oxidative stress and inflammation and decreased the severity of lung injury caused by pneumoperitoneum. CONCLUSIONS: By increasing NO bioavailability and suppressing oxidative stress and inflammation, pretreatment with an arginase inhibitor may protect against lung injury caused by pneumoperitoneum. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 129 | - |
dc.relation.isPartOf | BMC ANESTHESIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Aminocaproates/administration & dosage | - |
dc.subject.MESH | Aminocaproates/pharmacology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Arginase/antagonists & inhibitors* | - |
dc.subject.MESH | Boron Compounds/administration & dosage | - |
dc.subject.MESH | Boron Compounds/pharmacology* | - |
dc.subject.MESH | Cytokines/metabolism | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Enzyme Inhibitors/administration & dosage | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology | - |
dc.subject.MESH | Inflammation/etiology | - |
dc.subject.MESH | Inflammation/prevention & control* | - |
dc.subject.MESH | Injections, Subcutaneous | - |
dc.subject.MESH | Lung Injury/etiology | - |
dc.subject.MESH | Lung Injury/prevention & control | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Malondialdehyde/metabolism | - |
dc.subject.MESH | Nitric Oxide/metabolism | - |
dc.subject.MESH | Oxidative Stress/drug effects* | - |
dc.subject.MESH | Pneumoperitoneum/complications* | - |
dc.subject.MESH | Pneumoperitoneum/drug therapy | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.title | The effects of arginase inhibitor on lung oxidative stress and inflammation caused by pneumoperitoneum in rats | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anesthesiology (마취통증의학) | - |
dc.contributor.googleauthor | Jin Sun Cho | - |
dc.contributor.googleauthor | Young Jun Oh | - |
dc.contributor.googleauthor | Ok Soo Kim | - |
dc.contributor.googleauthor | Sungwon Na | - |
dc.identifier.doi | 10.1186/s12871-015-0112-y | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01232 | - |
dc.contributor.localId | A02389 | - |
dc.contributor.localId | A03914 | - |
dc.relation.journalcode | J00349 | - |
dc.identifier.eissn | 1471-2253 | - |
dc.identifier.pmid | 26415531 | - |
dc.subject.keyword | Arginase | - |
dc.subject.keyword | Inflammation | - |
dc.subject.keyword | Lung injury | - |
dc.subject.keyword | Nitric oxide | - |
dc.subject.keyword | Oxidative stress | - |
dc.subject.keyword | Pneumoperitoneum | - |
dc.contributor.alternativeName | Na, Sung Won | - |
dc.contributor.alternativeName | Oh, Young Jun | - |
dc.contributor.alternativeName | Cho, Jin Sun | - |
dc.contributor.affiliatedAuthor | Na, Sung Won | - |
dc.contributor.affiliatedAuthor | Oh, Young Jun | - |
dc.contributor.affiliatedAuthor | Cho, Jin Sun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 15 | - |
dc.citation.startPage | 129 | - |
dc.identifier.bibliographicCitation | BMC ANESTHESIOLOGY, Vol.15 : 129, 2015 | - |
dc.identifier.rimsid | 30582 | - |
dc.type.rims | ART | - |
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