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Efficient endodermal induction of human adipose stem cells using various concentrations of Activin A for hepatic differentiation

DC Field Value Language
dc.contributor.author김경식-
dc.contributor.author황호경-
dc.date.accessioned2016-02-04T11:46:39Z-
dc.date.available2016-02-04T11:46:39Z-
dc.date.issued2015-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/141179-
dc.description.abstractActivin A, which is a signaling molecule similar to Nodal, rapidly promotes endoderm induction of both embryonic stem (ES) cells and MSCs. Protocols for hepatic induction exhibit differences in efficiency and reproducibility depending on the specific methods or sources of MSCs. We characterized the effects of Activin A concentration on induction efficiency during hepatic differentiation of MSCs. Human MSCs (hMSCs) were differentiated into a hepatic lineage via a three-step protocol. Cells were first cultured in fetal bovine serum-free MSCs conditioned medium supplemented with Activin A (20, 50, or 100 ng/mL) for 3 days followed by treatment with additional agents. RT-PCR analysis, immunocytochemistry assays, periodic acid and Schiff's solution staining, and ELISAs were performed to confirm hepatic induction of hMSCs. Expression of genes related to the primitive foregut endoderm was observed in cells treated with higher concentration of Activin A. Gene expression related to functional primitive hepatocytes was observed in the early phases of hepatic differentiation. During the early period of the differentiation protocol, greater albumin secretion was observed when cells were treated with higher concentrations of Activin A.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1178~1184-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHActivins/administration & dosage*-
dc.subject.MESHAdipocytes/cytology*-
dc.subject.MESHAdipocytes/drug effects-
dc.subject.MESHAdipocytes/physiology-
dc.subject.MESHCell Differentiation/drug effects-
dc.subject.MESHCell Differentiation/physiology-
dc.subject.MESHCells, Cultured-
dc.subject.MESHEndoderm/cytology*-
dc.subject.MESHEndoderm/drug effects*-
dc.subject.MESHEndoderm/physiology-
dc.subject.MESHHepatocytes/cytology*-
dc.subject.MESHHepatocytes/drug effects-
dc.subject.MESHHepatocytes/physiology-
dc.subject.MESHHumans-
dc.subject.MESHMesenchymal Stromal Cells/cytology*-
dc.subject.MESHMesenchymal Stromal Cells/drug effects-
dc.subject.MESHMesenchymal Stromal Cells/physiology-
dc.titleEfficient endodermal induction of human adipose stem cells using various concentrations of Activin A for hepatic differentiation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorSang Woo Lee-
dc.contributor.googleauthorSeon Ok Min-
dc.contributor.googleauthorSeon Young Bak-
dc.contributor.googleauthorHo Kyoung Hwang-
dc.contributor.googleauthorKyung Sik Kim-
dc.identifier.doi10.1016/j.bbrc.2015.07.100-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00299-
dc.contributor.localIdA04497-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid26208453-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X15303302-
dc.subject.keywordActivin A-
dc.subject.keywordCell differentiation-
dc.subject.keywordEndoderm-
dc.subject.keywordHepatocyte-
dc.subject.keywordMesenchymal stem cells-
dc.contributor.alternativeNameKim, Kyung Sik-
dc.contributor.alternativeNameHwang, Ho Kyoung-
dc.contributor.affiliatedAuthorKim, Kyung Sik-
dc.contributor.affiliatedAuthorHwang, Ho Kyoung-
dc.rights.accessRightsnot free-
dc.citation.volume464-
dc.citation.number4-
dc.citation.startPage1178-
dc.citation.endPage1184-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.464(4) : 1178-1184, 2015-
dc.identifier.rimsid29347-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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