Cited 9 times in
Efficient endodermal induction of human adipose stem cells using various concentrations of Activin A for hepatic differentiation
DC Field | Value | Language |
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dc.contributor.author | 김경식 | - |
dc.contributor.author | 황호경 | - |
dc.date.accessioned | 2016-02-04T11:46:39Z | - |
dc.date.available | 2016-02-04T11:46:39Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141179 | - |
dc.description.abstract | Activin A, which is a signaling molecule similar to Nodal, rapidly promotes endoderm induction of both embryonic stem (ES) cells and MSCs. Protocols for hepatic induction exhibit differences in efficiency and reproducibility depending on the specific methods or sources of MSCs. We characterized the effects of Activin A concentration on induction efficiency during hepatic differentiation of MSCs. Human MSCs (hMSCs) were differentiated into a hepatic lineage via a three-step protocol. Cells were first cultured in fetal bovine serum-free MSCs conditioned medium supplemented with Activin A (20, 50, or 100 ng/mL) for 3 days followed by treatment with additional agents. RT-PCR analysis, immunocytochemistry assays, periodic acid and Schiff's solution staining, and ELISAs were performed to confirm hepatic induction of hMSCs. Expression of genes related to the primitive foregut endoderm was observed in cells treated with higher concentration of Activin A. Gene expression related to functional primitive hepatocytes was observed in the early phases of hepatic differentiation. During the early period of the differentiation protocol, greater albumin secretion was observed when cells were treated with higher concentrations of Activin A. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1178~1184 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Activins/administration & dosage* | - |
dc.subject.MESH | Adipocytes/cytology* | - |
dc.subject.MESH | Adipocytes/drug effects | - |
dc.subject.MESH | Adipocytes/physiology | - |
dc.subject.MESH | Cell Differentiation/drug effects | - |
dc.subject.MESH | Cell Differentiation/physiology | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Endoderm/cytology* | - |
dc.subject.MESH | Endoderm/drug effects* | - |
dc.subject.MESH | Endoderm/physiology | - |
dc.subject.MESH | Hepatocytes/cytology* | - |
dc.subject.MESH | Hepatocytes/drug effects | - |
dc.subject.MESH | Hepatocytes/physiology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mesenchymal Stromal Cells/cytology* | - |
dc.subject.MESH | Mesenchymal Stromal Cells/drug effects | - |
dc.subject.MESH | Mesenchymal Stromal Cells/physiology | - |
dc.title | Efficient endodermal induction of human adipose stem cells using various concentrations of Activin A for hepatic differentiation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | Sang Woo Lee | - |
dc.contributor.googleauthor | Seon Ok Min | - |
dc.contributor.googleauthor | Seon Young Bak | - |
dc.contributor.googleauthor | Ho Kyoung Hwang | - |
dc.contributor.googleauthor | Kyung Sik Kim | - |
dc.identifier.doi | 10.1016/j.bbrc.2015.07.100 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00299 | - |
dc.contributor.localId | A04497 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 26208453 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X15303302 | - |
dc.subject.keyword | Activin A | - |
dc.subject.keyword | Cell differentiation | - |
dc.subject.keyword | Endoderm | - |
dc.subject.keyword | Hepatocyte | - |
dc.subject.keyword | Mesenchymal stem cells | - |
dc.contributor.alternativeName | Kim, Kyung Sik | - |
dc.contributor.alternativeName | Hwang, Ho Kyoung | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Sik | - |
dc.contributor.affiliatedAuthor | Hwang, Ho Kyoung | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 464 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1178 | - |
dc.citation.endPage | 1184 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.464(4) : 1178-1184, 2015 | - |
dc.identifier.rimsid | 29347 | - |
dc.type.rims | ART | - |
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