Cited 76 times in
In-depth N-glycome profiling of paired colorectal cancer and non-tumorigenic tissues reveals cancer-, stage- and EGFR-specific protein N-glycosylation
DC Field | Value | Language |
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dc.contributor.author | 김호근 | - |
dc.contributor.author | 박철근 | - |
dc.date.accessioned | 2016-02-04T11:44:36Z | - |
dc.date.available | 2016-02-04T11:44:36Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0959-6658 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141100 | - |
dc.description.abstract | Glycomics may assist in uncovering the structure-function relationships of protein glycosylation and identify glycoprotein markers in colorectal cancer (CRC) research. Herein, we performed label-free quantitative glycomics on a carbon-liquid chromatography-tandem mass spectrometry-based analytical platform to accurately profile the N-glycosylation changes associated with CRC malignancy. N-Glycome profiling was performed on isolated membrane proteomes of paired tumorigenic and adjacent non-tumorigenic colon tissues from a cohort of five males (62.6 ± 13.1 y.o.) suffering from colorectal adenocarcinoma. The CRC tissues were typed according to their epidermal growth factor receptor (EGFR) status by western blotting and immunohistochemistry. Detailed N-glycan characterization and relative quantitation identified an extensive structural heterogeneity with a total of 91 N-glycans. CRC-specific N-glycosylation phenotypes were observed including an overrepresentation of high mannose, hybrid and paucimannosidic type N-glycans and an under-representation of complex N-glycans (P < 0.05). Sialylation, in particular α2,6-sialylation, was significantly higher in CRC tumors relative to non-tumorigenic tissues, whereas α2,3-sialylation was down-regulated (P < 0.05). CRC stage-specific N-glycosylation was detected by high α2,3-sialylation and low bisecting β1,4-GlcNAcylation and Lewis-type fucosylation in mid-late relative to early stage CRC. Interestingly, a novel link between the EGFR status and the N-glycosylation was identified using hierarchical clustering of the N-glycome profiles. EGFR-specific N-glycan signatures included high bisecting β1,4-GlcNAcylation and low α2,3-sialylation (both P < 0.05) relative to EGFR-negative CRC tissues. This is the first study to correlate CRC stage and EGFR status with specific N-glycan features, thus advancing our understanding of the mechanisms causing the biomolecular deregulation associated with CRC. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | GLYCOBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenocarcinoma/metabolism* | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Carbohydrate Conformation | - |
dc.subject.MESH | Carbohydrate Sequence | - |
dc.subject.MESH | Colorectal Neoplasms/metabolism* | - |
dc.subject.MESH | Glycoproteins/metabolism* | - |
dc.subject.MESH | Glycosylation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Protein Processing, Post-Translational | - |
dc.subject.MESH | Proteome/metabolism | - |
dc.subject.MESH | Receptor, Epidermal Growth Factor/metabolism* | - |
dc.subject.MESH | Sialic Acids/chemistry | - |
dc.subject.MESH | Sialic Acids/metabolism | - |
dc.title | In-depth N-glycome profiling of paired colorectal cancer and non-tumorigenic tissues reveals cancer-, stage- and EGFR-specific protein N-glycosylation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | Manveen K Sethi | - |
dc.contributor.googleauthor | Hoguen Kim | - |
dc.contributor.googleauthor | Cheol Keun Park | - |
dc.contributor.googleauthor | Mark S Baker | - |
dc.contributor.googleauthor | Young-Ki Paik | - |
dc.contributor.googleauthor | Nicolle H Packer | - |
dc.contributor.googleauthor | William S Hancock | - |
dc.contributor.googleauthor | Susan Fanayan | - |
dc.contributor.googleauthor | Morten Thaysen-Andersen | - |
dc.identifier.doi | 10.1093/glycob/cwv042 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01183 | - |
dc.relation.journalcode | J00950 | - |
dc.identifier.eissn | 1460-2423 | - |
dc.identifier.pmid | 26085185 | - |
dc.identifier.url | http://glycob.oxfordjournals.org/content/25/10/1064.long | - |
dc.subject.keyword | Colorectal cancer | - |
dc.subject.keyword | EGFR | - |
dc.subject.keyword | N-glycosylation | - |
dc.subject.keyword | glycome | - |
dc.subject.keyword | glycomics | - |
dc.contributor.alternativeName | Kim, Ho Keun | - |
dc.contributor.affiliatedAuthor | Kim, Ho Keun | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 25 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1064 | - |
dc.citation.endPage | 1078 | - |
dc.identifier.bibliographicCitation | GLYCOBIOLOGY, Vol.25(10) : 1064-1078, 2015 | - |
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