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Differential Protein Expression in Congenital and Acquired Cholesteatomas

DC FieldValueLanguage
dc.contributor.author김성헌-
dc.contributor.author최재영-
dc.date.accessioned2016-02-04T11:43:02Z-
dc.date.available2016-02-04T11:43:02Z-
dc.date.issued2015-
dc.identifier.issn1932-6203-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/141041-
dc.description.abstractCongenital cholesteatomas are epithelial lesions that present as an epithelial pearl behind an intact eardrum. Congenital and acquired cholesteatomas progress quite differently from each other and progress patterns can provide clues about the unique origin and pathogenesis of the abnormality. However, the exact pathogenic mechanisms by which cholesteatomas develop remain unknown. In this study, key proteins that directly affect cholesteatoma pathogenesis are investigated with proteomics and immunohistochemistry. Congenital cholesteatoma matrices and retroauricular skin were harvested during surgery in 4 patients diagnosed with a congenital cholesteatoma. Tissue was also harvested from the retraction pocket in an additional 2 patients during middle ear surgery. We performed 2-dimensional (2D) electrophoresis to detect and analyze spots that are expressed only in congenital cholesteatoma and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS) to separate proteins by molecular weight. Protein expression was confirmed by immunohistochemical staining. The image analysis of 2D electrophoresis showed that 4 congenital cholesteatoma samples had very similar protein expression patterns and that 127 spots were exclusively expressed in congenital cholesteatomas. Of these 127 spots, 10 major spots revealed the presence of titin, forkhead transcription activator homolog (FKH 5-3), plectin 1, keratin 10, and leucine zipper protein 5 by MALDI-TOF/MS analysis. Immunohistochemical staining showed that FKH 5-3 and titin were expressed in congenital cholesteatoma matrices, but not in acquired cholesteatomas. Our study shows that protein expression patterns are completely different in congenital cholesteatomas, acquired cholesteatomas, and skin. Moreover, non-epithelial proteins, including FKH 5-3 and titin, were unexpectedly expressed in congenital cholesteatoma tissue. Our data indicates that congenital cholesteatoma origins may differ from those of acquired cholesteatomas, which originate from retraction pocket epithelia.-
dc.description.statementOfResponsibilityopen-
dc.format.extente0137011-
dc.relation.isPartOfPLoS One-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHChild, Preschool-
dc.subject.MESHCholesteatoma/congenital*-
dc.subject.MESHCholesteatoma/metabolism*-
dc.subject.MESHCholesteatoma/pathology-
dc.subject.MESHConnectin/metabolism-
dc.subject.MESHFemale-
dc.subject.MESHForkhead Transcription Factors/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHProteomics-
dc.subject.MESHSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization-
dc.titleDifferential Protein Expression in Congenital and Acquired Cholesteatomas-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학)-
dc.contributor.googleauthorSeung-Ho Shin-
dc.contributor.googleauthorMei Huang-
dc.contributor.googleauthorSung Huhn Kim-
dc.contributor.googleauthorJae Young Choi-
dc.identifier.doi10.1371/journal.pone.0137011-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04173-
dc.contributor.localIdA00589-
dc.relation.journalcodeJ02540-
dc.identifier.pmid26335306-
dc.contributor.alternativeNameKim, Sung Huhn-
dc.contributor.alternativeNameChoi, Jae Young-
dc.contributor.affiliatedAuthorChoi, Jae Young-
dc.contributor.affiliatedAuthorKim, Sung Huhn-
dc.rights.accessRightsfree-
dc.citation.volume10-
dc.citation.number9-
dc.citation.startPagee0137011-
dc.identifier.bibliographicCitationPLoS One, Vol.10(9) : e0137011, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

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