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DNAJB1 negatively regulates MIG6 to promote epidermal growth factor receptor signaling

DC FieldValueLanguage
dc.contributor.author박수연-
dc.contributor.author서재성-
dc.contributor.author윤호근-
dc.contributor.author유정윤-
dc.date.accessioned2016-02-04T11:39:23Z-
dc.date.available2016-02-04T11:39:23Z-
dc.date.issued2015-
dc.identifier.issn0167-4889-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140908-
dc.description.abstractMitogen-inducible gene 6 (MIG6) is a tumor suppressor implicated in the development of human cancers; however, the regulatory mechanisms of MIG6 remain unknown. Here, using a yeast two-hybrid screen, we identified DnaJ homolog subfamily B member I (DNAJB1) as a novel MIG6-interacting protein. We found that DNAJB1 binds to and decreases MIG6 protein, but not mRNA, levels. DNAJB1 overexpression dosage-dependently decreased MIG6 protein levels. Conversely, DNAJB1 knockdown increased MIG6 protein levels. DNAJB1 destabilizes MIG6 by enhancing K48-linked ubiquitination of MIG6. However, knocking-down of DNAJB1 reduced the ubiquitination of MIG6. DNAJB1 positively regulates the epidermal growth factor receptors (EGFR) signaling pathway via destabilization of MIG6; however, DNAJB1 knockdown diminishes activation of EGFR signaling as well as elevation of MIG6. Importantly, the increased levels of MIG6 by DNAJB1 knockdown greatly enhanced the gefitinib sensitivity in A549 cells. Thus, our study provides a new molecular mechanism to regulate EGFR signaling through modulation of MIG6 by DNAJB1 as a negative regulator.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2722~2730-
dc.relation.isPartOfBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdaptor Proteins, Signal Transducing/genetics-
dc.subject.MESHAdaptor Proteins, Signal Transducing/metabolism*-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHGene Knockdown Techniques-
dc.subject.MESHHSP40 Heat-Shock Proteins/genetics-
dc.subject.MESHHSP40 Heat-Shock Proteins/metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHProtein Binding-
dc.subject.MESHReceptor, Epidermal Growth Factor/genetics-
dc.subject.MESHReceptor, Epidermal Growth Factor/metabolism*-
dc.subject.MESHSignal Transduction/physiology*-
dc.subject.MESHTumor Suppressor Proteins/genetics-
dc.subject.MESHTumor Suppressor Proteins/metabolism*-
dc.subject.MESHUbiquitination/physiology*-
dc.titleDNAJB1 negatively regulates MIG6 to promote epidermal growth factor receptor signaling-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorSoo-Yeon Park-
dc.contributor.googleauthorHyo-Kyoung Choi-
dc.contributor.googleauthorJae Sung Seo-
dc.contributor.googleauthorJung-Yoon Yoo-
dc.contributor.googleauthorJae-Wook Jeong-
dc.contributor.googleauthorYoungsok Choi-
dc.contributor.googleauthorKyung-Chul Choi-
dc.contributor.googleauthorHo-Geun Yoon-
dc.identifier.doi10.1016/j.bbamcr.2015.07.024-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01900-
dc.contributor.localIdA02625-
dc.contributor.localIdA01534-
dc.contributor.localIdA02502-
dc.relation.journalcodeJ00291-
dc.identifier.eissn1878-2434-
dc.identifier.pmid26239118-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S016748891500261X-
dc.subject.keywordDNAJB1-
dc.subject.keywordEGFR-
dc.subject.keywordGefitinib-
dc.subject.keywordLung cancer-
dc.subject.keywordMIG6-
dc.contributor.alternativeNamePark, Soo Yeon-
dc.contributor.alternativeNameSeo, Jae Sung-
dc.contributor.alternativeNameYoon, Ho Geun-
dc.contributor.affiliatedAuthorSeo, Jae Sung-
dc.contributor.affiliatedAuthorYoon, Ho Geun-
dc.contributor.affiliatedAuthorPark, Soo Yeon-
dc.contributor.affiliatedAuthorYoo, Jung Yoon-
dc.contributor.affiliatedAuthor유정윤-
dc.rights.accessRightsnot free-
dc.citation.volume1853-
dc.citation.number10 Pt A-
dc.citation.startPage2722-
dc.citation.endPage2730-
dc.identifier.bibliographicCitationBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, Vol.1853(10 Pt A) : 2722-2730, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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