Cited 18 times in
Single high-dose irradiation aggravates eosinophil-mediated fibrosis through IL-33 secreted from impaired vessels in the skin compared to fractionated irradiation
DC Field | Value | Language |
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dc.contributor.author | 이은정 | - |
dc.contributor.author | 조재호 | - |
dc.contributor.author | 최원훈 | - |
dc.contributor.author | 김준원 | - |
dc.date.accessioned | 2016-02-04T11:37:46Z | - |
dc.date.available | 2016-02-04T11:37:46Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140847 | - |
dc.description.abstract | We have revealed in a porcine skin injury model that eosinophil recruitment was dose-dependently enhanced by a single high-dose irradiation. In this study, we investigated the underlying mechanism of eosinophil-associated skin fibrosis and the effect of high-dose-per-fraction radiation. The dorsal skin of a mini-pig was divided into two sections containing 4-cm(2) fields that were irradiated with 30 Gy in a single fraction or 5 fractions and biopsied regularly over 14 weeks. Eosinophil-related Th2 cytokines such as interleukin (IL)-4, IL-5, and C-C motif chemokine-11 (CCL11/eotaxin) were evaluated by quantitative real-time PCR. RNA-sequencing using 30 Gy-irradiated mouse skin and functional assays in a co-culture system of THP-1 and irradiated-human umbilical vein endothelial cells (HUVECs) were performed to investigate the mechanism of eosinophil-mediated radiation fibrosis. Single high-dose-per-fraction irradiation caused pronounced eosinophil accumulation, increased profibrotic factors collagen and transforming growth factor-β, enhanced production of eosinophil-related cytokines including IL-4, IL-5, CCL11, IL-13, and IL-33, and reduced vessels compared with 5-fraction irradiation. IL-33 notably increased in pig and mouse skin vessels after single high-dose irradiation of 30 Gy, as well as in irradiated HUVECs following 12 Gy. Blocking IL-33 suppressed the migration ability of THP-1 cells and cytokine secretion in a co-culture system of THP-1 cells and irradiated HUVECs. Hence, high-dose-per-fraction irradiation appears to enhance eosinophil-mediated fibrotic responses, and IL-33 may be a key molecule operating in eosinophil-mediated fibrosis in high-dose-per fraction irradiated skin. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 20~26 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies/pharmacology | - |
dc.subject.MESH | Cell Movement/drug effects | - |
dc.subject.MESH | Cell Movement/radiation effects | - |
dc.subject.MESH | Chemokine CCL11/genetics | - |
dc.subject.MESH | Chemokine CCL11/metabolism | - |
dc.subject.MESH | Coculture Techniques | - |
dc.subject.MESH | Dose-Response Relationship, Radiation | - |
dc.subject.MESH | Eosinophils/drug effects | - |
dc.subject.MESH | Eosinophils/metabolism | - |
dc.subject.MESH | Eosinophils/pathology | - |
dc.subject.MESH | Eosinophils/radiation effects* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Fibrosis | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Human Umbilical Vein Endothelial Cells/drug effects | - |
dc.subject.MESH | Human Umbilical Vein Endothelial Cells/metabolism | - |
dc.subject.MESH | Human Umbilical Vein Endothelial Cells/pathology | - |
dc.subject.MESH | Human Umbilical Vein Endothelial Cells/radiation effects* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-33 | - |
dc.subject.MESH | Interleukin-4/genetics | - |
dc.subject.MESH | Interleukin-4/metabolism | - |
dc.subject.MESH | Interleukin-5/genetics | - |
dc.subject.MESH | Interleukin-5/metabolism | - |
dc.subject.MESH | Interleukins/antagonists & inhibitors | - |
dc.subject.MESH | Interleukins/genetics | - |
dc.subject.MESH | Interleukins/secretion* | - |
dc.subject.MESH | Macrophages/drug effects | - |
dc.subject.MESH | Macrophages/metabolism | - |
dc.subject.MESH | Macrophages/pathology | - |
dc.subject.MESH | Macrophages/radiation effects* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Radiation Dosage | - |
dc.subject.MESH | Skin/blood supply | - |
dc.subject.MESH | Skin/drug effects | - |
dc.subject.MESH | Skin/pathology* | - |
dc.subject.MESH | Skin/radiation effects* | - |
dc.subject.MESH | Swine | - |
dc.subject.MESH | Transforming Growth Factor beta/genetics | - |
dc.subject.MESH | Transforming Growth Factor beta/metabolism | - |
dc.subject.MESH | X-Rays | - |
dc.title | Single high-dose irradiation aggravates eosinophil-mediated fibrosis through IL-33 secreted from impaired vessels in the skin compared to fractionated irradiation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | Eun-Jung Lee | - |
dc.contributor.googleauthor | Jun Won Kim | - |
dc.contributor.googleauthor | Hyun Yoo | - |
dc.contributor.googleauthor | Woori Kwak | - |
dc.contributor.googleauthor | Won Hoon Choi | - |
dc.contributor.googleauthor | Seoae Cho | - |
dc.contributor.googleauthor | Yu Jeong Choi | - |
dc.contributor.googleauthor | Yoon-Jin Lee | - |
dc.contributor.googleauthor | Jaeho Cho | - |
dc.identifier.doi | 10.1016/j.bbrc.2015.05.081 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03047 | - |
dc.contributor.localId | A03901 | - |
dc.contributor.localId | A04129 | - |
dc.contributor.localId | A00958 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 26047701 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X15300073 | - |
dc.subject.keyword | Eosinophil-mediated fibrosis | - |
dc.subject.keyword | Interleukin-33 | - |
dc.subject.keyword | Radiotherapy | - |
dc.subject.keyword | Skin injury | - |
dc.contributor.alternativeName | Lee, Eun Jung | - |
dc.contributor.alternativeName | Cho, Jae Ho | - |
dc.contributor.alternativeName | Choi, Won Hoon | - |
dc.contributor.alternativeName | Kim, Jun Won | - |
dc.contributor.affiliatedAuthor | Lee, Eun Jung | - |
dc.contributor.affiliatedAuthor | Cho, Jae Ho | - |
dc.contributor.affiliatedAuthor | Choi, Won Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Jun Won | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 464 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 20 | - |
dc.citation.endPage | 26 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.464(1) : 20-26, 2015 | - |
dc.identifier.rimsid | 30346 | - |
dc.type.rims | ART | - |
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