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Single high-dose irradiation aggravates eosinophil-mediated fibrosis through IL-33 secreted from impaired vessels in the skin compared to fractionated irradiation

DC FieldValueLanguage
dc.contributor.author이은정-
dc.contributor.author조재호-
dc.contributor.author최원훈-
dc.contributor.author김준원-
dc.date.accessioned2016-02-04T11:37:46Z-
dc.date.available2016-02-04T11:37:46Z-
dc.date.issued2015-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140847-
dc.description.abstractWe have revealed in a porcine skin injury model that eosinophil recruitment was dose-dependently enhanced by a single high-dose irradiation. In this study, we investigated the underlying mechanism of eosinophil-associated skin fibrosis and the effect of high-dose-per-fraction radiation. The dorsal skin of a mini-pig was divided into two sections containing 4-cm(2) fields that were irradiated with 30 Gy in a single fraction or 5 fractions and biopsied regularly over 14 weeks. Eosinophil-related Th2 cytokines such as interleukin (IL)-4, IL-5, and C-C motif chemokine-11 (CCL11/eotaxin) were evaluated by quantitative real-time PCR. RNA-sequencing using 30 Gy-irradiated mouse skin and functional assays in a co-culture system of THP-1 and irradiated-human umbilical vein endothelial cells (HUVECs) were performed to investigate the mechanism of eosinophil-mediated radiation fibrosis. Single high-dose-per-fraction irradiation caused pronounced eosinophil accumulation, increased profibrotic factors collagen and transforming growth factor-β, enhanced production of eosinophil-related cytokines including IL-4, IL-5, CCL11, IL-13, and IL-33, and reduced vessels compared with 5-fraction irradiation. IL-33 notably increased in pig and mouse skin vessels after single high-dose irradiation of 30 Gy, as well as in irradiated HUVECs following 12 Gy. Blocking IL-33 suppressed the migration ability of THP-1 cells and cytokine secretion in a co-culture system of THP-1 cells and irradiated HUVECs. Hence, high-dose-per-fraction irradiation appears to enhance eosinophil-mediated fibrotic responses, and IL-33 may be a key molecule operating in eosinophil-mediated fibrosis in high-dose-per fraction irradiated skin.-
dc.description.statementOfResponsibilityopen-
dc.format.extent20~26-
dc.relation.isPartOfBiochemical and Biophysical Research Communications-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleSingle high-dose irradiation aggravates eosinophil-mediated fibrosis through IL-33 secreted from impaired vessels in the skin compared to fractionated irradiation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Radiation Oncology (방사선종양학)-
dc.contributor.googleauthorEun-Jung Lee-
dc.contributor.googleauthorJun Won Kim-
dc.contributor.googleauthorHyun Yoo-
dc.contributor.googleauthorWoori Kwak-
dc.contributor.googleauthorWon Hoon Choi-
dc.contributor.googleauthorSeoae Cho-
dc.contributor.googleauthorYu Jeong Choi-
dc.contributor.googleauthorYoon-Jin Lee-
dc.contributor.googleauthorJaeho Cho-
dc.identifier.doi10.1016/j.bbrc.2015.05.081-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03047-
dc.contributor.localIdA03901-
dc.contributor.localIdA04129-
dc.contributor.localIdA00958-
dc.relation.journalcodeJ00281-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X15300073-
dc.contributor.alternativeNameLee, Eun Jung-
dc.contributor.alternativeNameCho, Jae Ho-
dc.contributor.alternativeNameChoi, Won Hoon-
dc.contributor.alternativeNameKim, Jun Won-
dc.contributor.affiliatedAuthorLee, Eun Jung-
dc.contributor.affiliatedAuthorCho, Jae Ho-
dc.contributor.affiliatedAuthorChoi, Won Hoon-
dc.contributor.affiliatedAuthorKim, Jun Won-
dc.rights.accessRightsnot free-
dc.citation.volume464-
dc.citation.number1-
dc.citation.startPage20-
dc.citation.endPage26-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, Vol.464(1) : 20-26, 2015-
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실)

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