Cited 2 times in
A Gene and Neural Stem Cell Therapy Platform Based on Neuronal Cell Type-Inducible Gene Overexpression
DC Field | Value | Language |
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dc.contributor.author | 윤도흠 | - |
dc.contributor.author | 하윤 | - |
dc.date.accessioned | 2016-02-04T11:33:33Z | - |
dc.date.available | 2016-02-04T11:33:33Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140695 | - |
dc.description.abstract | PURPOSE: Spinal cord injury (SCI) is associated with permanent neurological damage, and treatment thereof with a single modality often does not provide sufficient therapeutic outcomes. Therefore, a strategy that combines two or more techniques might show better therapeutic effects. MATERIALS AND METHODS: In this study, we designed a combined treatment strategy based on neural stem cells (NSCs) introduced via a neuronal cell type-inducible transgene expression system (NSE::) controlled by a neuron-specific enolase (NSE) promoter to maximize therapeutic efficiency and neuronal differentiation. The luciferase gene was chosen to confirm whether this combined system was working properly prior to using a therapeutic gene. The luciferase expression levels of NSCs introduced via the neuronal cell type-inducible luciferase expression system (NSE::Luci) or via a general luciferase expressing system (SV::Luci) were measured and compared in vitro and in vivo. RESULTS: NSCs introduced via the neuronal cell type-inducible luciferase expressing system (NSE::Luci-NSCs) showed a high level of luciferase expression, compared to NSCs introduced via a general luciferase expressing system (SV::Luci-NSCs). Interestingly, the luciferase expression level of NSE::Luci-NSCs increased greatly after differentiation into neurons. CONCLUSION: We demonstrated that a neuronal cell type-inducible gene expression system is suitable for introducing NSCs in combined treatment strategies. We suggest that the proposed strategy may be a promising tool for the treatment of neurodegenerative disorders, including SCI. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1036~1043 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Differentiation/genetics | - |
dc.subject.MESH | Cell Differentiation/physiology | - |
dc.subject.MESH | Gene Expression* | - |
dc.subject.MESH | Gene Regulatory Networks | - |
dc.subject.MESH | Genetic Therapy* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Luciferases/genetics | - |
dc.subject.MESH | Luciferases/metabolism* | - |
dc.subject.MESH | Neural Stem Cells* | - |
dc.subject.MESH | Neurons/metabolism | - |
dc.subject.MESH | Phosphopyruvate Hydratase/metabolism | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Spinal Cord Injuries/therapy* | - |
dc.subject.MESH | Stem Cells/metabolism* | - |
dc.title | A Gene and Neural Stem Cell Therapy Platform Based on Neuronal Cell Type-Inducible Gene Overexpression | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학) | - |
dc.contributor.googleauthor | Jinsoo Oh | - |
dc.contributor.googleauthor | Youngsang You | - |
dc.contributor.googleauthor | Yeomin Yun | - |
dc.contributor.googleauthor | Hye-Lan Lee | - |
dc.contributor.googleauthor | Do Heum Yoon | - |
dc.contributor.googleauthor | Minhyung Lee | - |
dc.contributor.googleauthor | Yoon Ha | - |
dc.identifier.doi | 10.3349/ymj.2015.56.4.1036 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02546 | - |
dc.contributor.localId | A04255 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 26069128 | - |
dc.subject.keyword | Neuronal cell type-inducible transgene expression | - |
dc.subject.keyword | combined treatment strategy | - |
dc.subject.keyword | neural stem cells | - |
dc.subject.keyword | spinal cord injury | - |
dc.contributor.alternativeName | Yoon, Do Heum | - |
dc.contributor.alternativeName | Ha, Yoon | - |
dc.contributor.affiliatedAuthor | Yoon, Do Heum | - |
dc.contributor.affiliatedAuthor | Ha, Yoon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 56 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1036 | - |
dc.citation.endPage | 1043 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.56(4) : 1036-1043, 2015 | - |
dc.identifier.rimsid | 30252 | - |
dc.type.rims | ART | - |
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