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Quantitative proteomic analysis of paired colorectal cancer and non-tumorigenic tissues reveals signature proteins and perturbed pathways involved in CRC progression and metastasis

DC FieldValueLanguage
dc.contributor.author김호근-
dc.date.accessioned2016-02-04T11:32:27Z-
dc.date.available2016-02-04T11:32:27Z-
dc.date.issued2015-
dc.identifier.issn1874-3919-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140655-
dc.description.abstractModern proteomics has proven instrumental in our understanding of the molecular deregulations associated with the development and progression of cancer. Herein, we profile membrane-enriched proteome of tumor and adjacent normal tissues from eight CRC patients using label-free nanoLC-MS/MS-based quantitative proteomics and advanced pathway analysis. Of the 948 identified proteins, 184 proteins were differentially expressed (P<0.05, fold change>1.5) between the tumor and non-tumor tissue (69 up-regulated and 115 down-regulated in tumor tissues). The CRC tumor and non-tumor tissues clustered tightly in separate groups using hierarchical cluster analysis of the differentially expressed proteins, indicating a strong CRC-association of this proteome subset. Specifically, cancer associated proteins such as FN1, TNC, DEFA1, ITGB2, MLEC, CDH17, EZR and pathways including actin cytoskeleton and RhoGDI signaling were deregulated. Stage-specific proteome signatures were identified including up-regulated ribosomal proteins and down-regulated annexin proteins in early stage CRC. Finally, EGFR(+) CRC tissues showed an EGFR-dependent down-regulation of cell adhesion molecules, relative to EGFR(-) tissues. Taken together, this study provides a detailed map of the altered proteome and associated protein pathways in CRC, which enhances our mechanistic understanding of CRC biology and opens avenues for a knowledge-driven search for candidate CRC protein markers.-
dc.description.statementOfResponsibilityopen-
dc.format.extent54~67-
dc.relation.isPartOfJournal of Proteomics-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleQuantitative proteomic analysis of paired colorectal cancer and non-tumorigenic tissues reveals signature proteins and perturbed pathways involved in CRC progression and metastasis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pathology (병리학)-
dc.contributor.googleauthorManveen K. Sethi-
dc.contributor.googleauthorMorten Thaysen-Andersen-
dc.contributor.googleauthorHoguen Kim-
dc.contributor.googleauthorCheol Keun Park-
dc.contributor.googleauthorMark S. Baker-
dc.contributor.googleauthorNicolle H. Packer-
dc.contributor.googleauthorYoung Ki Paik-
dc.contributor.googleauthorWilliam S. Hancock-
dc.contributor.googleauthorSusan Fanayan-
dc.identifier.doi10.1016/j.jprot.2015.05.037-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01183-
dc.relation.journalcodeJ01721-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1874391915300373-
dc.contributor.alternativeNameKim, Ho Keun-
dc.contributor.affiliatedAuthorKim, Ho Keun-
dc.rights.accessRightsnot free-
dc.citation.volume126-
dc.citation.startPage54-
dc.citation.endPage67-
dc.identifier.bibliographicCitationJournal of Proteomics, Vol.126 : 54-67, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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