Cited 29 times in
Quantitative proteomic analysis of paired colorectal cancer and non-tumorigenic tissues reveals signature proteins and perturbed pathways involved in CRC progression and metastasis
DC Field | Value | Language |
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dc.contributor.author | 김호근 | - |
dc.contributor.author | 박철근 | - |
dc.date.accessioned | 2016-02-04T11:32:27Z | - |
dc.date.available | 2016-02-04T11:32:27Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1874-3919 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140655 | - |
dc.description.abstract | Modern proteomics has proven instrumental in our understanding of the molecular deregulations associated with the development and progression of cancer. Herein, we profile membrane-enriched proteome of tumor and adjacent normal tissues from eight CRC patients using label-free nanoLC-MS/MS-based quantitative proteomics and advanced pathway analysis. Of the 948 identified proteins, 184 proteins were differentially expressed (P<0.05, fold change>1.5) between the tumor and non-tumor tissue (69 up-regulated and 115 down-regulated in tumor tissues). The CRC tumor and non-tumor tissues clustered tightly in separate groups using hierarchical cluster analysis of the differentially expressed proteins, indicating a strong CRC-association of this proteome subset. Specifically, cancer associated proteins such as FN1, TNC, DEFA1, ITGB2, MLEC, CDH17, EZR and pathways including actin cytoskeleton and RhoGDI signaling were deregulated. Stage-specific proteome signatures were identified including up-regulated ribosomal proteins and down-regulated annexin proteins in early stage CRC. Finally, EGFR(+) CRC tissues showed an EGFR-dependent down-regulation of cell adhesion molecules, relative to EGFR(-) tissues. Taken together, this study provides a detailed map of the altered proteome and associated protein pathways in CRC, which enhances our mechanistic understanding of CRC biology and opens avenues for a knowledge-driven search for candidate CRC protein markers. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF PROTEOMICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Colorectal Neoplasms/metabolism* | - |
dc.subject.MESH | Colorectal Neoplasms/pathology | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Neoplasm Proteins/biosynthesis* | - |
dc.subject.MESH | Proteome/biosynthesis* | - |
dc.subject.MESH | Proteomics* | - |
dc.subject.MESH | Signal Transduction* | - |
dc.title | Quantitative proteomic analysis of paired colorectal cancer and non-tumorigenic tissues reveals signature proteins and perturbed pathways involved in CRC progression and metastasis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | Manveen K. Sethi | - |
dc.contributor.googleauthor | Morten Thaysen-Andersen | - |
dc.contributor.googleauthor | Hoguen Kim | - |
dc.contributor.googleauthor | Cheol Keun Park | - |
dc.contributor.googleauthor | Mark S. Baker | - |
dc.contributor.googleauthor | Nicolle H. Packer | - |
dc.contributor.googleauthor | Young Ki Paik | - |
dc.contributor.googleauthor | William S. Hancock | - |
dc.contributor.googleauthor | Susan Fanayan | - |
dc.identifier.doi | 10.1016/j.jprot.2015.05.037 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01183 | - |
dc.relation.journalcode | J01721 | - |
dc.identifier.eissn | 1876-7737 | - |
dc.identifier.pmid | 26054784 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1874391915300373 | - |
dc.subject.keyword | Colorectal cancer | - |
dc.subject.keyword | Epidermal growth factor receptor | - |
dc.subject.keyword | Fibronectin | - |
dc.subject.keyword | Label free shotgun proteomics | - |
dc.subject.keyword | Malectin | - |
dc.subject.keyword | Membrane proteins | - |
dc.contributor.alternativeName | Kim, Ho Keun | - |
dc.contributor.affiliatedAuthor | Kim, Ho Keun | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 126 | - |
dc.citation.startPage | 54 | - |
dc.citation.endPage | 67 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PROTEOMICS, Vol.126 : 54-67, 2015 | - |
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